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Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses
Yang, Jing-Yue; Cao, Da-Yong; Liu, Wen-Chao; Zhang, Hong-Mei; Teng, Zeng-Hui; Ren, Jun
Source PublicationCELLULAR IMMUNOLOGY
2006-03-01
Volume240Issue:1Pages:14-21
Indexed BySCI
Abstract

Dendritic cells (DCs) are professional antigen presenting cells that are being considered as potential immunotherapeutic agents to promote host immune responses against tumor antigens. The use of such modified antigen-presenting cells for research or therapeutic have been limited by several factors, including maintaining DCs in a highly activated state, efficient transduction and expression, stable expression, identification of appropriate tumor-associated antigens, and absence of unintended functional changes or cytotoxicity. In this study, the feasibility of using CD34-DCs for tumor immunotherapy after transduction with a recombinant adenovirus containing HBsAg gene (AdVHBsAg), an HCC-associated antigen, was investigated. The gene transfer with recombinant adenovirus vectors (AdV) can obtained high levels of stable expression of HBsAg and its efficiency was increased in a multiplicity of infection (MOI)-dependent manner. Moreover, the AdVHBsAg infection had no appreciable effect on apoptosis of DCs compared with that of mock-infected DCs. The T cell lines, primed by the recombinant AdVHBsAg-infected DCs in vitro, recognized HBsAg-expressing tumor cell lines in a human leukocyte antigen (HLA) class I-restricted manner, and evoked a higher CTL response, which indicated that high potent and specific antitumor immune response could be induced by AdVHBsAg DC vaccine. It may be a promising the therapeutic modality for the treatment of HBsAg-expressing tumors, and will be a foundation for further study on DC vaccines and gene therapy for HCC. (c) 2006 Elsevier Inc. All rights reserved.

KeywordDendritic Cells Cytotoxic t Lymphocytes Immunotherapy Liver Neoplasm
Subject Area临床医学
SubtypeArticle
DOI10.1016/j.cellimm.2006.06.005
WOS HeadingsScience & Technology
Language英语
WOS Research AreaCell Biology ; Immunology
WOS SubjectCell Biology ; Immunology
WOS KeywordNECROSIS-FACTOR-ALPHA ; HEPATOCELLULAR-CARCINOMA ; RECOMBINANT ADENOVIRUS ; ANTITUMOR IMMUNITY ; CORD-BLOOD ; VECTOR ; EFFICIENT ; MELANOMA ; CANCER ; TRANSDUCTION
WOS IDWOS:000240486100003
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.bjmu.edu.cn/handle/400002259/66621
Collection北京大学临床肿瘤学院
Affiliation1.Peking Univ, Sch Oncol, Beijing 100036, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Oncol, Xian 710032, Peoples R China
3.Fourth Mil Med Univ, Dept Hepatobil Surg, Xijing Hosp, Xian 710032, Peoples R China
4.Fourth Mil Med Univ, Fac Preclin Med, Dept Pharmacol, Xian 710032, Peoples R China
Recommended Citation
GB/T 7714
Yang, Jing-Yue,Cao, Da-Yong,Liu, Wen-Chao,et al. Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses[J]. CELLULAR IMMUNOLOGY,2006,240(1):14-21.
APA Yang, Jing-Yue,Cao, Da-Yong,Liu, Wen-Chao,Zhang, Hong-Mei,Teng, Zeng-Hui,&Ren, Jun.(2006).Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses.CELLULAR IMMUNOLOGY,240(1),14-21.
MLA Yang, Jing-Yue,et al."Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses".CELLULAR IMMUNOLOGY 240.1(2006):14-21.
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