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学科主题: 临床医学
题名:
Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses
作者: Yang, Jing-Yue; Cao, Da-Yong; Liu, Wen-Chao; Zhang, Hong-Mei; Teng, Zeng-Hui; Ren, Jun
关键词: dendritic cells ; cytotoxic T lymphocytes ; immunotherapy ; liver neoplasm
刊名: CELLULAR IMMUNOLOGY
发表日期: 2006-03-01
DOI: 10.1016/j.cellimm.2006.06.005
卷: 240, 期:1, 页:14-21
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology
研究领域[WOS]: Cell Biology ; Immunology
关键词[WOS]: NECROSIS-FACTOR-ALPHA ; HEPATOCELLULAR-CARCINOMA ; RECOMBINANT ADENOVIRUS ; ANTITUMOR IMMUNITY ; CORD-BLOOD ; VECTOR ; EFFICIENT ; MELANOMA ; CANCER ; TRANSDUCTION
英文摘要:

Dendritic cells (DCs) are professional antigen presenting cells that are being considered as potential immunotherapeutic agents to promote host immune responses against tumor antigens. The use of such modified antigen-presenting cells for research or therapeutic have been limited by several factors, including maintaining DCs in a highly activated state, efficient transduction and expression, stable expression, identification of appropriate tumor-associated antigens, and absence of unintended functional changes or cytotoxicity. In this study, the feasibility of using CD34-DCs for tumor immunotherapy after transduction with a recombinant adenovirus containing HBsAg gene (AdVHBsAg), an HCC-associated antigen, was investigated. The gene transfer with recombinant adenovirus vectors (AdV) can obtained high levels of stable expression of HBsAg and its efficiency was increased in a multiplicity of infection (MOI)-dependent manner. Moreover, the AdVHBsAg infection had no appreciable effect on apoptosis of DCs compared with that of mock-infected DCs. The T cell lines, primed by the recombinant AdVHBsAg-infected DCs in vitro, recognized HBsAg-expressing tumor cell lines in a human leukocyte antigen (HLA) class I-restricted manner, and evoked a higher CTL response, which indicated that high potent and specific antitumor immune response could be induced by AdVHBsAg DC vaccine. It may be a promising the therapeutic modality for the treatment of HBsAg-expressing tumors, and will be a foundation for further study on DC vaccines and gene therapy for HCC. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000240486100003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66621
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Beijing 100036, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Oncol, Xian 710032, Peoples R China
3.Fourth Mil Med Univ, Dept Hepatobil Surg, Xijing Hosp, Xian 710032, Peoples R China
4.Fourth Mil Med Univ, Fac Preclin Med, Dept Pharmacol, Xian 710032, Peoples R China

Recommended Citation:
Yang, Jing-Yue,Cao, Da-Yong,Liu, Wen-Chao,et al. Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses[J]. CELLULAR IMMUNOLOGY,2006,240(1):14-21.
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