IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses
Yang, Jing-Yue; Cao, Da-Yong; Liu, Wen-Chao; Zhang, Hong-Mei; Teng, Zeng-Hui; Ren, Jun
关键词dendritic cells cytotoxic T lymphocytes immunotherapy liver neoplasm
刊名CELLULAR IMMUNOLOGY
2006-03-01
DOI10.1016/j.cellimm.2006.06.005
240期:1页:14-21
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology
研究领域[WOS]Cell Biology ; Immunology
关键词[WOS]NECROSIS-FACTOR-ALPHA ; HEPATOCELLULAR-CARCINOMA ; RECOMBINANT ADENOVIRUS ; ANTITUMOR IMMUNITY ; CORD-BLOOD ; VECTOR ; EFFICIENT ; MELANOMA ; CANCER ; TRANSDUCTION
英文摘要

Dendritic cells (DCs) are professional antigen presenting cells that are being considered as potential immunotherapeutic agents to promote host immune responses against tumor antigens. The use of such modified antigen-presenting cells for research or therapeutic have been limited by several factors, including maintaining DCs in a highly activated state, efficient transduction and expression, stable expression, identification of appropriate tumor-associated antigens, and absence of unintended functional changes or cytotoxicity. In this study, the feasibility of using CD34-DCs for tumor immunotherapy after transduction with a recombinant adenovirus containing HBsAg gene (AdVHBsAg), an HCC-associated antigen, was investigated. The gene transfer with recombinant adenovirus vectors (AdV) can obtained high levels of stable expression of HBsAg and its efficiency was increased in a multiplicity of infection (MOI)-dependent manner. Moreover, the AdVHBsAg infection had no appreciable effect on apoptosis of DCs compared with that of mock-infected DCs. The T cell lines, primed by the recombinant AdVHBsAg-infected DCs in vitro, recognized HBsAg-expressing tumor cell lines in a human leukocyte antigen (HLA) class I-restricted manner, and evoked a higher CTL response, which indicated that high potent and specific antitumor immune response could be induced by AdVHBsAg DC vaccine. It may be a promising the therapeutic modality for the treatment of HBsAg-expressing tumors, and will be a foundation for further study on DC vaccines and gene therapy for HCC. (c) 2006 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000240486100003
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66621
Collection北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing 100036, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Oncol, Xian 710032, Peoples R China
3.Fourth Mil Med Univ, Dept Hepatobil Surg, Xijing Hosp, Xian 710032, Peoples R China
4.Fourth Mil Med Univ, Fac Preclin Med, Dept Pharmacol, Xian 710032, Peoples R China
Recommended Citation
GB/T 7714
Yang, Jing-Yue,Cao, Da-Yong,Liu, Wen-Chao,et al. Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses[J]. CELLULAR IMMUNOLOGY,2006,240(1):14-21.
APA Yang, Jing-Yue,Cao, Da-Yong,Liu, Wen-Chao,Zhang, Hong-Mei,Teng, Zeng-Hui,&Ren, Jun.(2006).Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses.CELLULAR IMMUNOLOGY,240(1),14-21.
MLA Yang, Jing-Yue,et al."Dendritic cell generated from CD34+hematopoietic progenitors can be transfected with adenovirus containing gene of HBsAg and induce antigen-specific cytotoxic T cell responses".CELLULAR IMMUNOLOGY 240.1(2006):14-21.
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