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学科主题: 临床医学
题名:
Activation of astrocytes by advanced glycation end products: cytokines induction and nitric oxide release
作者: Wang, Z; Li, DD; Liang, YY; Wang, DS; Cai, NS
关键词: advanced glycosylation end products ; astrocytes ; interleukine-1 ; tumor necrosis factor ; nitric oxide
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2002-11-01
卷: 23, 期:11, 页:974-980
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: OXIDATIVE STRESS ; ENDPRODUCTS ; DISEASE ; NEURONS ; INVIVO
英文摘要:

AIM: To investigate whether two kinds of in vitro prepared advanced glycation end products (AGE), Glu-BSA and Gal-BSA, could induce proinflammatory mediators IL-1beta and TNF-alpha, as well as oxidative stress and nitric oxide (NO), in astrocytes, thus contributing to brain injury. METHODS: Radioimmunoassay and RT-PCR technique were used to detect two cytokines′ level and existence of receptor for AGE (RAGE). DTNB reaction was used to measure reduced glutathione (GSH) level. NO content was assayed using Griess reagent provided by Promega. RESULTS: Enhanced protein levels of both cytokines in supernatants and cell lysates of astroglia cultures were detected after treated with AGE-BSA 1 g/L, especially Gal-BSA, for 72 h. The increases were also in a concentration-dependent manner. Changes in protein levels might be attributed to changes in transcriptional levels documented by semi-quantitative RT-PCR. Both AGE-BSA could also reduce astrocytic GSH and induce NO release. RAGE was detected in astrocytes. CONCLUSION: Enhanced levels of astrocytic proinflammatory mediators IL-1beta and TNF-alpha, and oxidative stress caused by AGE might contribute to, at least partially, the detrimental effects of

语种: 英语
WOS记录号: WOS:000179049300003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66622
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China
2.Peking Union Med Coll, Beijing 100050, Peoples R China
3.Peking Univ, Sch Oncol, Peking Inst Canc Res, Beijing 100034, Peoples R China

Recommended Citation:
Wang, Z,Li, DD,Liang, YY,et al. Activation of astrocytes by advanced glycation end products: cytokines induction and nitric oxide release[J]. ACTA PHARMACOLOGICA SINICA,2002,23(11):974-980.
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