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Activation of astrocytes by advanced glycation end products: cytokines induction and nitric oxide release
Wang, Z; Li, DD; Liang, YY; Wang, DS; Cai, NS
关键词advanced glycosylation end products astrocytes interleukine-1 tumor necrosis factor nitric oxide
刊名ACTA PHARMACOLOGICA SINICA
2002-11-01
23期:11页:974-980
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]OXIDATIVE STRESS ; ENDPRODUCTS ; DISEASE ; NEURONS ; INVIVO
英文摘要

AIM: To investigate whether two kinds of in vitro prepared advanced glycation end products (AGE), Glu-BSA and Gal-BSA, could induce proinflammatory mediators IL-1beta and TNF-alpha, as well as oxidative stress and nitric oxide (NO), in astrocytes, thus contributing to brain injury. METHODS: Radioimmunoassay and RT-PCR technique were used to detect two cytokines′ level and existence of receptor for AGE (RAGE). DTNB reaction was used to measure reduced glutathione (GSH) level. NO content was assayed using Griess reagent provided by Promega. RESULTS: Enhanced protein levels of both cytokines in supernatants and cell lysates of astroglia cultures were detected after treated with AGE-BSA 1 g/L, especially Gal-BSA, for 72 h. The increases were also in a concentration-dependent manner. Changes in protein levels might be attributed to changes in transcriptional levels documented by semi-quantitative RT-PCR. Both AGE-BSA could also reduce astrocytic GSH and induce NO release. RAGE was detected in astrocytes. CONCLUSION: Enhanced levels of astrocytic proinflammatory mediators IL-1beta and TNF-alpha, and oxidative stress caused by AGE might contribute to, at least partially, the detrimental effects of

语种英语
WOS记录号WOS:000179049300003
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被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66622
专题北京大学临床肿瘤学院
作者单位1.Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China
2.Peking Union Med Coll, Beijing 100050, Peoples R China
3.Peking Univ, Sch Oncol, Peking Inst Canc Res, Beijing 100034, Peoples R China
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Wang, Z,Li, DD,Liang, YY,et al. Activation of astrocytes by advanced glycation end products: cytokines induction and nitric oxide release[J]. ACTA PHARMACOLOGICA SINICA,2002,23(11):974-980.
APA Wang, Z,Li, DD,Liang, YY,Wang, DS,&Cai, NS.(2002).Activation of astrocytes by advanced glycation end products: cytokines induction and nitric oxide release.ACTA PHARMACOLOGICA SINICA,23(11),974-980.
MLA Wang, Z,et al."Activation of astrocytes by advanced glycation end products: cytokines induction and nitric oxide release".ACTA PHARMACOLOGICA SINICA 23.11(2002):974-980.
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