北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Elevated MMP expression in the MRL mouse retina creates a permissive environment for retinal regeneration
作者: Tucker, Budd1; Klassen, Henry2; Yang, Liu1,3; Chen, Dong Feng1; Young, Michael J.1
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2008-04-01
DOI: 10.1167/iovs.07-1058
卷: 49, 期:4, 页:1686-1695
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: CHONDROITIN SULFATE PROTEOGLYCAN ; FOCAL CEREBRAL-ISCHEMIA ; MATRIX METALLOPROTEINASES ; RAT RETINA ; LIMB REGENERATION ; RDS DEGENERATION ; DEFICIENT MICE ; SCARLESS HEART ; CD44 ; CELL
英文摘要:

PURPOSE. The MRL/MpJ ( healer) mouse is an established model for autoimmune studies and was recently identified as having a profound ability to undergo scarless regeneration of the tissue in the ear and heart. This regenerative capacity has been linked to elevated matrix metalloproteinase (MMP)-2 and -9 expression, giving this mouse the ability to degrade and remove inhibitory basement membrane molecules. Although elevated MMP expression has been reported in somatic tissues in this strain, little is known about MMP expression and the response to injury in the MRL/MpJ mouse retina. The purpose of this study was to investigate whether increased MMP expression and subsequent decreased inhibitory extracellular matrix molecule deposition in the MRL/MpJ mouse retina produces a permissive regenerative environment.

METHODS. Experiments were performed using 3- to 4- week-old MRL/MpJ, retinal degenerative (rd1), and C57BL/6 ( wild-type) mice. Western blotting, oligo-microarray, and immunohistochemical analyses were used to determine the level and location of MMP and extracellular matrix (ECM) protein expression. Retinal responses to injury were modeled by retinal detachment in vivo and in retinal explantation in vitro. The capacity of the retinal environment to support photoreceptor cell migration, integration, or regeneration was analyzed using hematoxylin-eosin, immunohistochemical staining, and cell counting.

RESULTS. Compared with C57BL/6J animals, MRL/MpJ mice exhibit elevated levels of MMP-2, - 9, and - 14 and decreased levels of the inhibitory proteins neurocan and CD44 within the retina. Although similar increases in MMP- 2, - 9, and CD44s (CD44 degradation product) were observed in the rd1 retina, elevated levels of the inhibitory ECM molecules (neurocan and CD44) remained. Thus, the MRL retinal environment, which expresses lower levels of inhibitory ECM molecules after injury, was more conducive to regeneration and enhanced photoreceptor integration in vitro than C57BL/6J or rd1 controls.

CONCLUSIONS. The MRL mouse retina shows elevated MMP expression and decreased levels of scar-related inhibitory molecules, which leads to a retinal environment that is more permissive for neural regeneration and cell integration after in vitro retinal explantation.

语种: 英语
WOS记录号: WOS:000254577200054
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66624
Appears in Collections:北京大学第一临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 1, Dept Ophthalmol, Beijing, Peoples R China
2.Harvard Univ, Sch Med, Schepens Eye Res Inst, Dept Ophthalmol, Boston, MA 02114 USA
3.Univ Calif Irvine, Sch Med, Dept Ophthalmol, Irvine, CA 92717 USA

Recommended Citation:
Tucker, Budd,Klassen, Henry,Yang, Liu,et al. Elevated MMP expression in the MRL mouse retina creates a permissive environment for retinal regeneration[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2008,49(4):1686-1695.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Tucker, Budd]'s Articles
[Klassen, Henry]'s Articles
[Yang, Liu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Tucker, Budd]‘s Articles
[Klassen, Henry]‘s Articles
[Yang, Liu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace