北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
A beta-40 Y10F Increases beta fibrils Formation but Attenuates the Neurotoxicity of Amyloid-beta Peptide
作者: Dai, Xueling1,2; Chang, Ping2; Liu, Wenjuan2; Xu, Ke3; Sun, Yaxuan2; Zhu, Shigong1; Jiang, Zhaofeng2
关键词: Alzheimer&prime ; s disease ; amyloid-beta peptide ; beta fibrils ; neurotoxicity
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
发表日期: 2012-05-01
DOI: 10.3390/ijms13055324
卷: 13, 期:5, 页:5324-5337
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: ALZHEIMERS-DISEASE ; A-BETA ; OXIDATIVE STRESS ; PROTEIN ; DITYROSINE ; BRAIN ; CYTOTOXICITY ; OLIGOMERS ; BINDING ; NEURODEGENERATION
英文摘要:

Alzheimer′s disease (AD) is characterized by the abnormal aggregation of amyloid-beta peptide (A beta) in extracellular deposits known as senile plaques. The tyrosine residue (Tyr-10) is believed to be important in A beta-induced neurotoxicity due to the formation of tyrosyl radicals. To reduce the likelihood of cross-linking, here we designed an A beta-40 analogue (A beta-40 Y10F) in which the tyrosine residue was substituted by a structurally similar residue, phenylalanine. The aggregation rate was determined by the Thioflavin T (ThT) assay, in which A beta-40 Y10F populated an ensemble of folded conformations much quicker and stronger than the wild type A beta. Biophysical tests subsequently confirmed the results of the ThT assay, suggesting the measured increase of beta-aggregation may arise predominantly from enhancement of hydrophobicity upon substitution and thus the propensity of intrinsic beta-sheet formation. Nevertheless, A beta-40 Y10F exhibited remarkably decreased neurotoxicity compared to A beta-40 which could be partly due to the reduced generation of hydrogen peroxide. These findings may lead to further understanding of the structural perturbation of A beta to its fibrillation.

语种: 英语
所属项目编号: 31071512 ; PHR20090514 ; SQKM201211417013
项目资助者: NSFC ; Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality, PHR (IHLB) ; Scientific Research Common Program of Beijing Municipal Commission of Education
WOS记录号: WOS:000306186200003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66628
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Dept Physiol & Pathophysiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China
3.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China

Recommended Citation:
Dai, Xueling,Chang, Ping,Liu, Wenjuan,et al. A beta-40 Y10F Increases beta fibrils Formation but Attenuates the Neurotoxicity of Amyloid-beta Peptide[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(5):5324-5337.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Dai, Xueling]'s Articles
[Chang, Ping]'s Articles
[Liu, Wenjuan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Dai, Xueling]‘s Articles
[Chang, Ping]‘s Articles
[Liu, Wenjuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace