IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Alteration of cholinergic, purinergic and sensory neurotransmission in the mouse colon of food allergy model
Leng, Yuxin1,2; Yamamoto, Takeshi1; Kadowaki, Makoto1
关键词Enteric nervous system Food allergy Mucosal mast cell Neuroimmune interaction ATP Non-cholinergic
刊名NEUROSCIENCE LETTERS
2008-11-21
DOI10.1016/j.neulet.2008.09.014
445期:3页:195-198
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者Ministry of Education, Culture, Sports Science and Technology of Japan ; Smoking Research Foundation ; Ministry of Education, Culture, Sports Science and Technology of Japan ; Smoking Research Foundation
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]MAST-CELLS ; RECEPTORS ; ATP ; NUCLEOTIDE ; EXPRESSION ; DIARRHEA ; JEJUNUM ; RELEASE ; MUCOSA ; NERVES
英文摘要

It is well known that intestinal anaphylaxis results in a disturbed intestinal motility. It is hypothesized that the chronic intestinal anaphylaxis-induced changes in the enteric neuronal circuitry cause intestinal motor malfunctions. However, detailed mechanisms largely remain unclear. The aim of this study was to investigate the pathophysiological role of ATP, which acts as a non-cholinergic neurotransmitter and a neuroimmune modulator, in a disturbed intestinal motility of food allergy (FA). The FA mice developed allergic diarrhea accompanied with chronic inflammation and mast cell hyperplasia in the colon. The excised proximal colons (PCs) were suspended in the longitudinal direction in organ baths. In the PCs precontracted by KCl (50mM), contractile responses to exogenous ATP (1 mM) were significantly (P<0.01) higher in FA mice (34.2% of KCl-induced precontractions) as compared to control mice (17.2%). Pretreatment with P2 purinoceptor antagonists [suramin and PPADs] significantly (P<0.01) reduced the ATP-evoked contractions to 7.7% and 1.5% in FA and control PCs, respectively. Furthermore, in the presence of inhibitors of cholinergic nerves and capsaicin-sensitive sensory nerves the electrical field stimulation (EFS: 10Hz)-evoked contractions were significantly (P<0.05) higher in FA mice (65.8% of EFS-evoked maximum contractions, n = 6) than those in control mice (47.9%, n = 6). In addition, cumulative application of suramin and PPADs further inhibited EFS-induced contractions by 21.7% in FA mice (n = 6, P<0.01) and 8.7% in control mice (n = 6, P<0.05). Thus, the present study suggests that the sustained alteration in cholinergic, purinergic and sensory neurotransmission contribute to the disturbed motility during the chronic intestinal anaphylaxis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种英语
所属项目编号20790472 ; 18590507
资助者Ministry of Education, Culture, Sports Science and Technology of Japan ; Smoking Research Foundation ; Ministry of Education, Culture, Sports Science and Technology of Japan ; Smoking Research Foundation
WOS记录号WOS:000260852900001
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66632
Collection北京大学第三临床医学院
作者单位1.Toyama Univ, Inst Natural Med, Div Gastrointestinal Pathophysiol, Toyama 9300194, Japan
2.Peking Univ, Hosp 3, Dept Digest Dis, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Leng, Yuxin,Yamamoto, Takeshi,Kadowaki, Makoto. Alteration of cholinergic, purinergic and sensory neurotransmission in the mouse colon of food allergy model[J]. NEUROSCIENCE LETTERS,2008,445(3):195-198.
APA Leng, Yuxin,Yamamoto, Takeshi,&Kadowaki, Makoto.(2008).Alteration of cholinergic, purinergic and sensory neurotransmission in the mouse colon of food allergy model.NEUROSCIENCE LETTERS,445(3),195-198.
MLA Leng, Yuxin,et al."Alteration of cholinergic, purinergic and sensory neurotransmission in the mouse colon of food allergy model".NEUROSCIENCE LETTERS 445.3(2008):195-198.
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