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学科主题: 口腔医学
题名:
Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate
作者: Wang, Xue-Dong1; Kou, Xiao-Xing1; He, Dan-Qing1; Zeng, Min-Min1; Meng, Zhen2; Bi, Rui-Yun2; Liu, Yan1; Zhang, Jie-Ni1; Gan, Ye-Hua2; Zhou, Yan-Heng1
刊名: PLOS ONE
发表日期: 2012-09-11
DOI: 10.1371/journal.pone.0045036
卷: 7, 期:9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: GENE-EXPRESSION ; FIBROBLAST DIFFERENTIATION ; OVARIECTOMIZED RATS ; CONDYLAR CARTILAGE ; DOWN-REGULATION ; IN-VIVO ; MODEL ; CHONDROCYTES ; DISORDERS ; INDUCTION
英文摘要:

Background: Osteoarthritis (OA) is an important subtype of temporomandibular disorders. A simple and reproducible animal model that mimics the histopathologic changes, both in the cartilage and subchondral bone, and clinical symptoms of temporomandibular joint osteoarthritis (TMJOA) would help in our understanding of its process and underlying mechanism.

Objective: To explore whether injection of monosodium iodoacetate (MIA) into the upper compartment of rat TMJ could induce OA-like lesions.

Methods: Female rats were injected with varied doses of MIA into the upper compartment and observed for up to 12 weeks. Histologic, radiographic, behavioral, and molecular changes in the TMJ were evaluated by light and electron microscopy, MicroCT scanning, head withdrawal threshold test, real-time PCR, immunohistochemistry, and TUNEL assay.

Results: The intermediate zone of the disc loosened by 1 day post-MIA injection and thinned thereafter. Injection of an MIA dose of 0.5 mg or higher induced typical OA-like lesions in the TMJ within 4 weeks. Condylar destruction presented in a time-dependent manner, including chondrocyte apoptosis in the early stages, subsequent cartilage matrix disorganization and subchondral bone erosion, fibrosis, subchondral bone sclerosis, and osteophyte formation in the late stages. Nociceptive responses increased in the early stages, corresponding to severe synovitis. Furthermore, chondrocyte apoptosis and an imbalance between anabolism and catabolism of cartilage and subchondral bone might account for the condylar destruction.

Conclusions: Multi-level data demonstrated a reliable and convenient rat model of TMJOA could be induced by MIA injection into the upper compartment. The model might facilitate TMJOA related researches.

语种: 英语
所属项目编号: 81070849 ; 2010DFB32980
项目资助者: National Natural Science Foundation of China ; China International Science and Technology Cooperation
WOS记录号: WOS:000308638700105
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66634
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Xue-Dong,Kou, Xiao-Xing,He, Dan-Qing,et al. Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate[J]. PLOS ONE,2012,7(9).
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