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学科主题口腔医学
Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate
Wang, Xue-Dong1; Kou, Xiao-Xing1; He, Dan-Qing1; Zeng, Min-Min1; Meng, Zhen2; Bi, Rui-Yun2; Liu, Yan1; Zhang, Jie-Ni1; Gan, Ye-Hua2; Zhou, Yan-Heng1
刊名PLOS ONE
2012-09-11
DOI10.1371/journal.pone.0045036
7期:9
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]GENE-EXPRESSION ; FIBROBLAST DIFFERENTIATION ; OVARIECTOMIZED RATS ; CONDYLAR CARTILAGE ; DOWN-REGULATION ; IN-VIVO ; MODEL ; CHONDROCYTES ; DISORDERS ; INDUCTION
英文摘要

Background: Osteoarthritis (OA) is an important subtype of temporomandibular disorders. A simple and reproducible animal model that mimics the histopathologic changes, both in the cartilage and subchondral bone, and clinical symptoms of temporomandibular joint osteoarthritis (TMJOA) would help in our understanding of its process and underlying mechanism.

Objective: To explore whether injection of monosodium iodoacetate (MIA) into the upper compartment of rat TMJ could induce OA-like lesions.

Methods: Female rats were injected with varied doses of MIA into the upper compartment and observed for up to 12 weeks. Histologic, radiographic, behavioral, and molecular changes in the TMJ were evaluated by light and electron microscopy, MicroCT scanning, head withdrawal threshold test, real-time PCR, immunohistochemistry, and TUNEL assay.

Results: The intermediate zone of the disc loosened by 1 day post-MIA injection and thinned thereafter. Injection of an MIA dose of 0.5 mg or higher induced typical OA-like lesions in the TMJ within 4 weeks. Condylar destruction presented in a time-dependent manner, including chondrocyte apoptosis in the early stages, subsequent cartilage matrix disorganization and subchondral bone erosion, fibrosis, subchondral bone sclerosis, and osteophyte formation in the late stages. Nociceptive responses increased in the early stages, corresponding to severe synovitis. Furthermore, chondrocyte apoptosis and an imbalance between anabolism and catabolism of cartilage and subchondral bone might account for the condylar destruction.

Conclusions: Multi-level data demonstrated a reliable and convenient rat model of TMJOA could be induced by MIA injection into the upper compartment. The model might facilitate TMJOA related researches.

语种英语
WOS记录号WOS:000308638700105
项目编号81070849 ; 2010DFB32980
资助机构National Natural Science Foundation of China ; China International Science and Technology Cooperation
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66634
专题北京大学口腔医学院_牙周科
北京大学口腔医学院_口腔正畸科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Wang, Xue-Dong,Kou, Xiao-Xing,He, Dan-Qing,et al. Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate[J]. PLOS ONE,2012,7(9).
APA Wang, Xue-Dong.,Kou, Xiao-Xing.,He, Dan-Qing.,Zeng, Min-Min.,Meng, Zhen.,...&Zhou, Yan-Heng.(2012).Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate.PLOS ONE,7(9).
MLA Wang, Xue-Dong,et al."Progression of Cartilage Degradation, Bone Resorption and Pain in Rat Temporomandibular Joint Osteoarthritis Induced by Injection of Iodoacetate".PLOS ONE 7.9(2012).
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