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学科主题: 临床医学
题名:
Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia
作者: Guo, Xin; Gui, Yao-Ting; Tang, Ai-Fa; Lu, Li-Hua; Gao, Xin; Cai, Zhi-Ming
关键词: VASA ; ejaculated spermatozoa ; oligozoospermia ; male infertility ; spermatogenesis
刊名: ASIAN JOURNAL OF ANDROLOGY
发表日期: 2007-05-01
DOI: 10.1111/j.1745-7262.2007.00253.x
卷: 9, 期:3, 页:339-344
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology ; Urology & Nephrology
研究领域[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]: EMBRYONIC STEM-CELLS ; GERM-CELLS ; DROSOPHILA-VASA ; HOMOLOG ; RNA ; FERTILITY ; SPERMATOGENESIS ; DERIVATION ; LINEAGE ; PROTEIN
英文摘要:

Aim: To detect the expression of VASA in human ejaculated spermatozoa, and to compare the expression of VASA between normozoospermic men and patients with oligozoospermia. Methods: Ejaculated spermatozoa were collected from normozoospermic men and patients with oligozoospermia by masturbation, and subsequently segregated through a discontinuous gradient of Percoll to obtain the spermatozoa. Reverse transcription polymerase chain reaction (RT-PCR), quantitative RT-PCR (QRT-PCR), immunoflurescence and Western blotting were used to detect the expression of VASA in mRNA and protein levels. Results: VASA mRNA was expressed in the ejaculated spermatozoa. QRT-PCR analysis showed that VASA mRNA level was approximately 5-fold higher in normozoospermic men than that in oligozoospermic men. Immunofluorescence and Western blotting analysis showed that VASA protein was located on the cytoplasmic membrane of heads and tails of spermatozoa, and its expression was significantly decreased in oligozoospermic men, which is similar to the result of QRT-PCR. Conclusion: The expression of VASA mRNA and protein was significantly decreased in the sperm of oligozoospermic men, which suggested the lower expression of the VASA gene might be associated with pathogenesis in some subtypes of male infertility and VASA could be used as a molecular marker for the diagnosis of male infertility.

语种: 英语
WOS记录号: WOS:000246396900007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66636
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Lab Male Reprod Med, Shenzhen 518036, Peoples R China
2.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Urosurg, Guangzhou 510630, Peoples R China

Recommended Citation:
Guo, Xin,Gui, Yao-Ting,Tang, Ai-Fa,et al. Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia[J]. ASIAN JOURNAL OF ANDROLOGY,2007,9(3):339-344.
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