IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia
Guo, Xin; Gui, Yao-Ting; Tang, Ai-Fa; Lu, Li-Hua; Gao, Xin; Cai, Zhi-Ming
关键词VASA ejaculated spermatozoa oligozoospermia male infertility spermatogenesis
刊名ASIAN JOURNAL OF ANDROLOGY
2007-05-01
DOI10.1111/j.1745-7262.2007.00253.x
9期:3页:339-344
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology ; Urology & Nephrology
研究领域[WOS]Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]EMBRYONIC STEM-CELLS ; GERM-CELLS ; DROSOPHILA-VASA ; HOMOLOG ; RNA ; FERTILITY ; SPERMATOGENESIS ; DERIVATION ; LINEAGE ; PROTEIN
英文摘要

Aim: To detect the expression of VASA in human ejaculated spermatozoa, and to compare the expression of VASA between normozoospermic men and patients with oligozoospermia. Methods: Ejaculated spermatozoa were collected from normozoospermic men and patients with oligozoospermia by masturbation, and subsequently segregated through a discontinuous gradient of Percoll to obtain the spermatozoa. Reverse transcription polymerase chain reaction (RT-PCR), quantitative RT-PCR (QRT-PCR), immunoflurescence and Western blotting were used to detect the expression of VASA in mRNA and protein levels. Results: VASA mRNA was expressed in the ejaculated spermatozoa. QRT-PCR analysis showed that VASA mRNA level was approximately 5-fold higher in normozoospermic men than that in oligozoospermic men. Immunofluorescence and Western blotting analysis showed that VASA protein was located on the cytoplasmic membrane of heads and tails of spermatozoa, and its expression was significantly decreased in oligozoospermic men, which is similar to the result of QRT-PCR. Conclusion: The expression of VASA mRNA and protein was significantly decreased in the sperm of oligozoospermic men, which suggested the lower expression of the VASA gene might be associated with pathogenesis in some subtypes of male infertility and VASA could be used as a molecular marker for the diagnosis of male infertility.

语种英语
WOS记录号WOS:000246396900007
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66636
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Hosp, Lab Male Reprod Med, Shenzhen 518036, Peoples R China
2.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Urosurg, Guangzhou 510630, Peoples R China
推荐引用方式
GB/T 7714
Guo, Xin,Gui, Yao-Ting,Tang, Ai-Fa,et al. Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia[J]. ASIAN JOURNAL OF ANDROLOGY,2007,9(3):339-344.
APA Guo, Xin,Gui, Yao-Ting,Tang, Ai-Fa,Lu, Li-Hua,Gao, Xin,&Cai, Zhi-Ming.(2007).Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia.ASIAN JOURNAL OF ANDROLOGY,9(3),339-344.
MLA Guo, Xin,et al."Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia".ASIAN JOURNAL OF ANDROLOGY 9.3(2007):339-344.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Guo, Xin]的文章
[Gui, Yao-Ting]的文章
[Tang, Ai-Fa]的文章
百度学术
百度学术中相似的文章
[Guo, Xin]的文章
[Gui, Yao-Ting]的文章
[Tang, Ai-Fa]的文章
必应学术
必应学术中相似的文章
[Guo, Xin]的文章
[Gui, Yao-Ting]的文章
[Tang, Ai-Fa]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。