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学科主题: 药学
题名:
Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry
作者: Zhang, Jia-Yu1; Li, Ning1; Che, Yan-Yun1; Zhang, Yun1; Liang, Shen-Xuan2; Zhao, Ming-Bo1; Jiang, Yong1; Tu, Peng-Fei1
关键词: HPLC-DAD-ESI-MS/MS ; Polymethoxylated flavonoids (PMFs) ; Characterization ; Murraya paniculata
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2011-12-15
DOI: 10.1016/j.jpba.2011.08.019
卷: 56, 期:5, 页:950-961
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: FRUCTUS-AURANTII ; CELL-LINES ; FLAVANONE ; CONSTITUENTS ; CHALCONE ; EXTRACTS ; PEEL
英文摘要:

A sensitive HPLC-DAD-ESI-MS/MS method was established to screen and identify the polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata (L.) Jack. 16 PMF standards were first to be analyzed in positive mode by the CID-MS/MS. For polymethoxylated flavones, the fragments of [M+H-n x 15](+) produced by loss of one or more methyl radicals from the protonated molecule, as well as [M+H-16](+), [M+H-28](+), [M+H-29](+), [M+H-31](+), [M+H-33](+), [M+H-43](+), [M+H-44](+), [M+H-46](+). and [M+H-61](+) fragment ions were detected, which could be taken as their diagnostic characters. For polymethoxylated flavanones and chalcones, their [M+H](+) ions usually underwent RDA cleavage fragmentation of the C-ring prior to the similar loss of diagnostic fragment ions as polymethoxylated flavones, which could be adopted as a shortcut to distinguish them from ordinary flavones rapidly. For the PMF glycosides, the neutral loss of the similar fragments with polymethoxylated flavones from their [aglycone+H](+) could be adopted as a simple method to screen them out from complex mixture. Based on these characterizations of PMFs and the results of EIC-MS/MS experiment, 70 PMFs including 45 flavones, 17 flavanones or chalcones and 8 PMFs glycosides were screened out from the complex extract of the leaves of M. paniculata. Among them, 16 compounds were unambiguously identified by comparison with reference substances. The results indicated that the developed analysis method could be employed as a rapid, effective technique for structural characterization of PMFs. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2009ZX09308-04 ; 2009ZX09311-004
项目资助者: Research Platform for Quality Standard of TCMs and Information System Building ; National S &amp ; T Major Project-Created Major New dugs Projects
WOS记录号: WOS:000295304100013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66644
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Capital Med Univ, Sch Chem Biol & Pharmaceut Sci, Beijing 100069, Peoples R China

Recommended Citation:
Zhang, Jia-Yu,Li, Ning,Che, Yan-Yun,et al. Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2011,56(5):950-961.
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