IR@PKUHSC  > 北京大学药学院
学科主题药学
Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry
Zhang, Jia-Yu1; Li, Ning1; Che, Yan-Yun1; Zhang, Yun1; Liang, Shen-Xuan2; Zhao, Ming-Bo1; Jiang, Yong1; Tu, Peng-Fei1
关键词HPLC-DAD-ESI-MS/MS Polymethoxylated flavonoids (PMFs) Characterization Murraya paniculata
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2011-12-15
DOI10.1016/j.jpba.2011.08.019
56期:5页:950-961
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
资助者Research Platform for Quality Standard of TCMs and Information System Building ; National S &amp ; T Major Project-Created Major New dugs Projects ; Research Platform for Quality Standard of TCMs and Information System Building ; National S &amp ; T Major Project-Created Major New dugs Projects
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]FRUCTUS-AURANTII ; CELL-LINES ; FLAVANONE ; CONSTITUENTS ; CHALCONE ; EXTRACTS ; PEEL
英文摘要

A sensitive HPLC-DAD-ESI-MS/MS method was established to screen and identify the polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata (L.) Jack. 16 PMF standards were first to be analyzed in positive mode by the CID-MS/MS. For polymethoxylated flavones, the fragments of [M+H-n x 15](+) produced by loss of one or more methyl radicals from the protonated molecule, as well as [M+H-16](+), [M+H-28](+), [M+H-29](+), [M+H-31](+), [M+H-33](+), [M+H-43](+), [M+H-44](+), [M+H-46](+). and [M+H-61](+) fragment ions were detected, which could be taken as their diagnostic characters. For polymethoxylated flavanones and chalcones, their [M+H](+) ions usually underwent RDA cleavage fragmentation of the C-ring prior to the similar loss of diagnostic fragment ions as polymethoxylated flavones, which could be adopted as a shortcut to distinguish them from ordinary flavones rapidly. For the PMF glycosides, the neutral loss of the similar fragments with polymethoxylated flavones from their [aglycone+H](+) could be adopted as a simple method to screen them out from complex mixture. Based on these characterizations of PMFs and the results of EIC-MS/MS experiment, 70 PMFs including 45 flavones, 17 flavanones or chalcones and 8 PMFs glycosides were screened out from the complex extract of the leaves of M. paniculata. Among them, 16 compounds were unambiguously identified by comparison with reference substances. The results indicated that the developed analysis method could be employed as a rapid, effective technique for structural characterization of PMFs. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
所属项目编号2009ZX09308-04 ; 2009ZX09311-004
资助者Research Platform for Quality Standard of TCMs and Information System Building ; National S &amp ; T Major Project-Created Major New dugs Projects ; Research Platform for Quality Standard of TCMs and Information System Building ; National S &amp ; T Major Project-Created Major New dugs Projects
WOS记录号WOS:000295304100013
引用统计
被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66644
专题北京大学药学院
作者单位1.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Capital Med Univ, Sch Chem Biol & Pharmaceut Sci, Beijing 100069, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Jia-Yu,Li, Ning,Che, Yan-Yun,et al. Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2011,56(5):950-961.
APA Zhang, Jia-Yu.,Li, Ning.,Che, Yan-Yun.,Zhang, Yun.,Liang, Shen-Xuan.,...&Tu, Peng-Fei.(2011).Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,56(5),950-961.
MLA Zhang, Jia-Yu,et al."Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 56.5(2011):950-961.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Jia-Yu]的文章
[Li, Ning]的文章
[Che, Yan-Yun]的文章
百度学术
百度学术中相似的文章
[Zhang, Jia-Yu]的文章
[Li, Ning]的文章
[Che, Yan-Yun]的文章
必应学术
必应学术中相似的文章
[Zhang, Jia-Yu]的文章
[Li, Ning]的文章
[Che, Yan-Yun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。