IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
T-cell epitopes in Severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS
Wang, YD; Sin, WYF; Xu, GB; Yang, HH; Wong, TY; Pang, XW; He, XY; Zhang, HG; Ng, JNL; Cheng, CSS; Lu, J; Meng, L; Yang, RF; Lai, ST; Guo, ZH; Xie, Y; Chen, WF
刊名JOURNAL OF VIROLOGY
2004-06-01
DOI10.1128/JVI.78.11.5612-5618.2004
78期:11页:5612-5618
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]MAJOR HISTOCOMPATIBILITY COMPLEX ; AMINOPEPTIDASE-N CD13 ; CLASS-I ; PEPTIDES ; IMMUNOGLOBULIN ; IDENTIFICATION ; INFECTION ; BIOLOGY ; REGIONS ; HLA-A2
英文摘要

The immunogenicity of HLA-A2-restricted T-cell epitopes in the S protein of the Severe acute respiratory syndrome coronavirus (SARS-CoV) and of human coronavirus strain 229e (HCoV-229e) was analyzed for the elicitation of a T-cell immune response in donors who had fully recovered from SARS-CoV infection. We employed online database analysis to compare the differences in the amino acid sequences of the homologous T epitopes of HCoV-229e and SARS-CoV. The identified T-cell epitope peptides were synthesized, and their binding affinities for HLA-A2 were validated and compared in the T2 cell system. The immunogenicity of all these peptides was assessed by using T cells obtained from donors who had fully recovered from SARS-CoV infection and from healthy donors with no history of SARS-CoV infection. HLA-A2 typing by indirect immunofluorescent antibody staining showed that 51.6% of SARS-CoV-infected patients were HLA-A2 positive. Online database analysis and the T2 cell binding test disclosed that the number of HLA-A2-restricted immunogenic epitopes of the S protein of SARS-CoV was decreased or even lost in comparison with the homologous sequences of the S protein of HCoV-229e. Among the peptides used in the study, the affinity of peptides from HCoV-229e (H77 and H881) and peptides from SARS-CoV (S978 and S1203) for binding to HLA-A2 was higher than that of other sequences. The gamma interferon (IFN-gamma) release Elispot assay revealed that only SARS-CoV-specific peptides S1203 and S978 induced a high frequency of IFN-gamma-secreting T-cell response in HLA-A2(+) donors who had fully recovered from SARS-CoV infection; such a T-cell epitope-specific response was not observed in HLA-A2(+) healthy donors or in HLA-A2(-) donors who had been infected with SARS-CoV after full recovery. Thus, T-cell epitopes S1203 and S978 are immunogenic and elicit an overt specific T-cell response in HIA-A2(+) SARS-CoV-infected patients.

语种英语
WOS记录号WOS:000221513400009
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66645
Collection北京大学基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
2.Princess Margaret Hosp, Hong Kong, Hong Kong, Peoples R China
3.Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China
4.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China
5.Peking Univ, Hosp 1, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Wang, YD,Sin, WYF,Xu, GB,et al. T-cell epitopes in Severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS[J]. JOURNAL OF VIROLOGY,2004,78(11):5612-5618.
APA Wang, YD.,Sin, WYF.,Xu, GB.,Yang, HH.,Wong, TY.,...&Chen, WF.(2004).T-cell epitopes in Severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS.JOURNAL OF VIROLOGY,78(11),5612-5618.
MLA Wang, YD,et al."T-cell epitopes in Severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS".JOURNAL OF VIROLOGY 78.11(2004):5612-5618.
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