IR@PKUHSC  > 北京大学第一临床医学院  > 消化科
学科主题临床医学
Distribution of gyrA mutations in fluoroquinolone-resistant Helicobacter pylori strains
Wang, Li-Hui; Cheng, Hong; Hu, Fu-Lian; Li, Jiang
关键词Helicobacter pylori Antibiotic resistance Quinolones Mutation gyrA
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2010-05-14
DOI10.3748/wjg.v16.i18.2272
16期:18页:2272-2277
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]ESCHERICHIA-COLI ; CONSENSUS REPORT ; TRIPLE THERAPY ; ERADICATION ; INFECTION ; LEVOFLOXACIN ; MOXIFLOXACIN ; PREVALENCE ; MANAGEMENT ; EFFICACY
英文摘要

AIM: To investigate the resistance of Helicobacter pylori (H. pylon) to ciprofloxacin (CIP), levofloxacin (LVX) and moxifloxacin (MOX) in the Beijing area and to elucidate the resistance mechanisms.

METHODS: Seventy-nine H. pylori clinical strains, isolated from patients who had undergone upper gastrointestinal endoscopy in Peking University First Hospital from 2007 to 2009, were tested for their susceptibility to CIF, LVX and MOX using the if-test method. H. pylori strain 26695 was included in the susceptibility testing as a control strain. According to the minimal inhibitory concentration (MIC) values, a strain was classified as resistant to CIP, LVX or MOX when the MIC was > 1 mu g/mL. We amplified by polymerase chain reaction (PCR) and sequenced the quinolone resistance-determining regions of the gyrA and gyrB genes from 29 quinolone-resistant and 16 quinolone-susceptible H. pylori strains selected at random.

RESULTS: In this study, the resistance rates of H. pylon to CIP, LVX or MOX were 55.7% (44/79), and the primary resistance rates were 26.6% (21/79). Patients with secondary resistance had received LVX in previous eradication treatments, but not MOX or CIP. Forty-five strains, including 29 CIP, LVX or MOX-resistant strains (MIC: 1.5-32 mu g/mL) and 16 susceptible strains, were selected randomly from the 79 strains and used in PCR analysis. Among these 45 strains, 27 resistant strains had mutations in the gyrA gene, including 11 strains with mutations corresponding to Asp-91 (MIC: 2-32 mu g/mL), one of which also had a mutation corresponding to Val-150, and 16 strains had mutations at Asn-87 (MIC: 4-32 mu g/mL), three of which also had mutations corresponding to Arg-140 or Val-150. In addition, Arg-140, Val-150 or Ala-97 mutations were separately detected in three susceptible strains. Analysis of the gyrB gene showed that one strain of low resistance had a mutation corresponding to Ser-457 that coexisted with an Asp-91 mutation. There was a significant difference in the occurrence of mutations in the gyrA gene between CIP, LVX and MOX-resistant and -susceptible strains (P < 0.05), but 2 resistant strains were found to possess no quinolone resistance-determining region mutations.

CONCLUSION: Resistance is primarily mediated through point mutations in gyrA. Whether other mechanisms are responsible for resistance in strains without mutations in the QRDR should be detected. (C) 2010 Baishideng. All rights reserved.

语种英语
WOS记录号WOS:000277696500010
项目编号2005-1008
资助机构Beijing Medicine Research and Development Fund
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66647
专题北京大学第一临床医学院_消化科
作者单位Peking Univ First Hosp, Dept Gastroenterol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Wang, Li-Hui,Cheng, Hong,Hu, Fu-Lian,et al. Distribution of gyrA mutations in fluoroquinolone-resistant Helicobacter pylori strains[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(18):2272-2277.
APA Wang, Li-Hui,Cheng, Hong,Hu, Fu-Lian,&Li, Jiang.(2010).Distribution of gyrA mutations in fluoroquinolone-resistant Helicobacter pylori strains.WORLD JOURNAL OF GASTROENTEROLOGY,16(18),2272-2277.
MLA Wang, Li-Hui,et al."Distribution of gyrA mutations in fluoroquinolone-resistant Helicobacter pylori strains".WORLD JOURNAL OF GASTROENTEROLOGY 16.18(2010):2272-2277.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Li-Hui]的文章
[Cheng, Hong]的文章
[Hu, Fu-Lian]的文章
百度学术
百度学术中相似的文章
[Wang, Li-Hui]的文章
[Cheng, Hong]的文章
[Hu, Fu-Lian]的文章
必应学术
必应学术中相似的文章
[Wang, Li-Hui]的文章
[Cheng, Hong]的文章
[Hu, Fu-Lian]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。