IR@PKUHSC  > 北京大学第二临床医学院  > 眼科
学科主题临床医学
Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells
Wu, Hui-Juan; Li, Xiao-Xin; Dong, Jian-Qiang; Pei, Wei-Hua; Chen, Hong-Da
关键词retinal pigment epithelium (RPE) subretinal microphotodiode array (MPDA) biocompatibility primary culture microporous filter
刊名GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
2007
DOI10.1007/s00417-006-0296-4
245期:1页:135-142
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]PIGMENT EPITHELIAL-CELLS ; ANNEXIN-V ; EXPRESSION ; ARPE-19 ; LINE
英文摘要

Background: Subretinal microphotodiode array (MPDA) is a type of visual prosthesis used for the implantation in the subretinal space of patients with progressive photoreceptor cell loss. The present study aimed to evaluate the effect of materials for MPDA on the viability, apoptosis and barrier function of cultured pig retinal pigment epithelium (RPE) cells.

Methods: Primary culture of pig RPE cells was performed and 24 pig eyes were used to start RPE culture. The third passage of the cultures was plated on different materials for MPDA and MPDAs. The tetrazolium dye-reduction assay (MTT) was used to determine RPE cell viability. Flow cytometry was measured to indicate the apoptosis rates of RPE cells on different materials. RPE cells were also cultured on microporous filters, and the transepithelial resistance and permeability of the experimental molecule were measured to determine the barrier function.

Results: The data from all the methods indicated no significant difference between the materials groups and the control group, and the materials tested showed good biocompatibility.

Conclusions: The materials for MPDA used in the present study had no direct toxicity to the RPE cells and did not release harmful soluble factors that affected the barrier function of RPE in vitro.

语种英语
WOS记录号WOS:000242461600017
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66653
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Dept Pathol, Peoples Hosp, Beijing, Peoples R China
2.Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Wu, Hui-Juan,Li, Xiao-Xin,Dong, Jian-Qiang,et al. Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells[J]. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,2007,245(1):135-142.
APA Wu, Hui-Juan,Li, Xiao-Xin,Dong, Jian-Qiang,Pei, Wei-Hua,&Chen, Hong-Da.(2007).Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells.GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,245(1),135-142.
MLA Wu, Hui-Juan,et al."Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured RPE cells".GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY 245.1(2007):135-142.
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