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Adeno-associated virus vector-mediated triple gene transfer of dopamine synthetic enzymes
Fan, DS; Shen, Y; Kang, DX; Nakano, I; Ozawa, K
关键词dopamine GTP cyclohydrolase I adeno-associated virus
刊名CHINESE MEDICAL JOURNAL
2001-12-01
114期:12页:1276-1279
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]GTP CYCLOHYDROLASE-I ; AMINO-ACID DECARBOXYLASE ; TYROSINE-HYDROXYLASE ; BEHAVIORAL RECOVERY ; MESSENGER-RNA ; CDNA-CLONE ; THERAPY ; RATS
英文摘要

Objective To explore triple gene transfer of dopamine synthetic enzymes with separate adeno-associated virus (AAV) vectors.

Methods The genes for dopamine synthetic enzymes, tyrosine hydroxylase (TH), aromatic L-amino acid decarboxylase (AADC), and GTP cyclohydrolase I ( GCH, an enzyme critical for tetrahydrobiopterin synthesis) were cotransduced into 293 cells with separate AAV vectors. Expressions of TH, AADC and GCH were detected by Western blot analysis. Intracellular dopamine level was assayed by highperformance liquid chromatography.

Results TH, AADC and GCH were effectively coexpressed in transduced cells with three separate AAV vectors, AAV-TH, AAV-AADC and AAV-GCH. Furthermore, the coexpression resulted in an effectively spontaneous dopamine production in cotransduced cells.

Conclusion The triple transduction of TH, AADC and GCH genes with separate AAV vectors is effective, which might be important to gene therapy for Parkinson′s disease.

语种英语
WOS记录号WOS:000173061100011
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66658
专题北京大学第三临床医学院_神经内科
作者单位1.Peking Univ, Hosp 3, Dept Neurol, Beijing 100083, Peoples R China
2.Jichi Med Sch, Ctr Mol Med, Dept Neurol, Minami Kawachi, Tochigi 3290498, Japan
3.Jichi Med Sch, Ctr Mol Med, Div Genet Therapeut, Minami Kawachi, Tochigi 3290498, Japan
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GB/T 7714
Fan, DS,Shen, Y,Kang, DX,et al. Adeno-associated virus vector-mediated triple gene transfer of dopamine synthetic enzymes[J]. CHINESE MEDICAL JOURNAL,2001,114(12):1276-1279.
APA Fan, DS,Shen, Y,Kang, DX,Nakano, I,&Ozawa, K.(2001).Adeno-associated virus vector-mediated triple gene transfer of dopamine synthetic enzymes.CHINESE MEDICAL JOURNAL,114(12),1276-1279.
MLA Fan, DS,et al."Adeno-associated virus vector-mediated triple gene transfer of dopamine synthetic enzymes".CHINESE MEDICAL JOURNAL 114.12(2001):1276-1279.
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