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学科主题: 临床医学
题名:
Neocartilage formation from predifferentiated human adipose derived stem cells in vivo
作者: Jin, Xiao-bing; Sun, Yong-sheng; Zhang, Ke; Wang, Jing; Ju, Xiao-dong; Lou, Si-quan
关键词: human adipose derived stem cell (hASC) ; human transforming growth factor beta2 (hTGF beta2) ; cartilage ; tissue engineering ; alginate
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2007-05-01
DOI: 10.1111/j.1745-7254.2007.00553.x
卷: 28, 期:5, 页:663-671
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: HUMAN BONE-MARROW ; MESENCHYMAL PROGENITOR CELLS ; ARTICULAR-CARTILAGE REPAIR ; GENE-EXPRESSION ; CHONDROGENIC DIFFERENTIATION ; STROMAL CELLS ; VITRO CHONDROGENESIS ; ALGINATE BEADS ; TISSUE ; CHONDROCYTES
英文摘要:

Aim: To examine the chondrogenic potential of human adipose derived stem cells (hASC) induced by human transforming growth factor beta2 (hTGF beta2) in vitro, and to investigate if predifferentiated hASC can produce neocartilage in vivo. Methods: hASC were isolated from subcutaneous adipose tissue and cultured in pellets with the addition of hTGF beta2. Chondrogenic differentiation was assayed by RT-PCR, Western blotting, toluidine blue staining, and immuno-histochemistry staining for collagen type II. For the in vivo study, intact induced cell pellets or the released cells embedded in alginate gel with different concentrations were implanted subcutaneously in nude mice. Specimens were harvested at different time points and carried with histological and immunohistochemistry examination to evaluate the cartilage formation. Results:RT-PCR analysis revealed that hASC produced aggrecan and collagen type II after 7 d of induction and continued throughout the culture period. This was also demonstrated by the Western blot analysis, positive staining of toluidine blue, and immunohistochemistry for collagen type II. After reseeding in the monolayer, the cells isolated from the pellets displayed a polygonal morphology compared with the primary spindle shape. hASC were released from the induced cell pellets when embedded in alginate gel (implanted cell concentration=5 x 10(6)/mL or higher). They produced neocartilage after 12 weeks in vivo culture; however, intact induced cell pellets implanted subcutaneously rapidly lost their differentiated phenotype. Conclusion: Chondrogenesis of hASC in vitro can be induced by combining pellet culture and hTGF beta2 treatment. Predifferentiated hASC embedded in alginate gel have the ability of producing neocartilage in vivo.

语种: 英语
WOS记录号: WOS:000246150800009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66659
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100083, Peoples R China
2.Fourth Mil Med Univ, Coll Stomatol, Xian 710032, Peoples R China

Recommended Citation:
Jin, Xiao-bing,Sun, Yong-sheng,Zhang, Ke,et al. Neocartilage formation from predifferentiated human adipose derived stem cells in vivo[J]. ACTA PHARMACOLOGICA SINICA,2007,28(5):663-671.
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