IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Genome Analyses of Single Human Oocytes
Hou, Yu1,2; Fan, Wei1,2,6; Yan, Liying1,2; Li, Rong1,2; Lian, Ying1,2; Huang, Jin1,2; Li, Jinsen1,2; Xu, Liya1,2; Tang, Fuchou1,2,7; Xie, X. Sunney1,2,3; Qiao, Jie1,2,4,5
刊名CELL
2013-12-19
DOI10.1016/j.cell.2013.11.040
155期:7页:1492-1506
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
资助者National Basic Research Program of China ; National Natural Science of China ; Beijing Municipal Science and Technology Commission ; Peking University ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Natural Science of China ; Beijing Municipal Science and Technology Commission ; Peking University ; National Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]PREIMPLANTATION GENETIC DIAGNOSIS ; MEIOTIC RECOMBINATION ; MATERNAL AGE ; CROSSOVER INTERFERENCE ; CHROMATID INTERFERENCE ; SPERM CELLS ; ANEUPLOIDY ; NONDISJUNCTION ; RATES ; RESOLUTION
英文摘要

Single-cell genome analyses of human oocytes are important for meiosis research and preimplantation genomic screening. However, the nonuniformity of single-cell whole-genome amplification hindered its use. Here, we demonstrate genome analyses of single human oocytes using multiple annealing and looping-based amplification cycle (MALBAC)-based sequencing technology. By sequencing the triads of the first and second polar bodies (PB1 and PB2) and the oocyte pronuclei from same female egg donors, we phase the genomes of these donors with detected SNPs and determine the crossover maps of their oocytes. Our data exhibit an expected crossover interference and indicate a weak chromatid interference. Further, the genome of the oocyte pronucleus, including information regarding aneuploidy and SNPs in disease-associated alleles, can be accurately deduced from the genomes of PB1 and PB2. The MALBAC-based preimplantation genomic screening in in vitro fertilization (IVF) enables accurate and cost-effective selection of normal fertilized eggs for embryo transfer.

语种英语
所属项目编号2011CB944500 ; 2012CB966704 ; 2011CB966303 ; 31230047 ; 31322037 ; 31271543 ; Z131100005213006 ; 81000275
资助者National Basic Research Program of China ; National Natural Science of China ; Beijing Municipal Science and Technology Commission ; Peking University ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Natural Science of China ; Beijing Municipal Science and Technology Commission ; Peking University ; National Natural Science Foundation of China
WOS记录号WOS:000328693300007
Citation statistics
Cited Times:116[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66660
Collection北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Coll Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Ctr Reprod Med, Beijing 100871, Peoples R China
3.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
4.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
5.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
6.Peking Tsinghua Ctr Life Sci, Beijing 100084, Peoples R China
7.Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Hou, Yu,Fan, Wei,Yan, Liying,et al. Genome Analyses of Single Human Oocytes[J]. CELL,2013,155(7):1492-1506.
APA Hou, Yu.,Fan, Wei.,Yan, Liying.,Li, Rong.,Lian, Ying.,...&Qiao, Jie.(2013).Genome Analyses of Single Human Oocytes.CELL,155(7),1492-1506.
MLA Hou, Yu,et al."Genome Analyses of Single Human Oocytes".CELL 155.7(2013):1492-1506.
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