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学科主题: 临床医学
题名:
Genome Analyses of Single Human Oocytes
作者: Hou, Yu1,2; Fan, Wei1,2,6; Yan, Liying1,2; Li, Rong1,2; Lian, Ying1,2; Huang, Jin1,2; Li, Jinsen1,2; Xu, Liya1,2; Tang, Fuchou1,2,7; Xie, X. Sunney1,2,3; Qiao, Jie1,2,4,5
刊名: CELL
发表日期: 2013-12-19
DOI: 10.1016/j.cell.2013.11.040
卷: 155, 期:7, 页:1492-1506
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: PREIMPLANTATION GENETIC DIAGNOSIS ; MEIOTIC RECOMBINATION ; MATERNAL AGE ; CROSSOVER INTERFERENCE ; CHROMATID INTERFERENCE ; SPERM CELLS ; ANEUPLOIDY ; NONDISJUNCTION ; RATES ; RESOLUTION
英文摘要:

Single-cell genome analyses of human oocytes are important for meiosis research and preimplantation genomic screening. However, the nonuniformity of single-cell whole-genome amplification hindered its use. Here, we demonstrate genome analyses of single human oocytes using multiple annealing and looping-based amplification cycle (MALBAC)-based sequencing technology. By sequencing the triads of the first and second polar bodies (PB1 and PB2) and the oocyte pronuclei from same female egg donors, we phase the genomes of these donors with detected SNPs and determine the crossover maps of their oocytes. Our data exhibit an expected crossover interference and indicate a weak chromatid interference. Further, the genome of the oocyte pronucleus, including information regarding aneuploidy and SNPs in disease-associated alleles, can be accurately deduced from the genomes of PB1 and PB2. The MALBAC-based preimplantation genomic screening in in vitro fertilization (IVF) enables accurate and cost-effective selection of normal fertilized eggs for embryo transfer.

语种: 英语
所属项目编号: 2011CB944500 ; 2012CB966704 ; 2011CB966303 ; 31230047 ; 31322037 ; 31271543 ; Z131100005213006 ; 81000275
项目资助者: National Basic Research Program of China ; National Natural Science of China ; Beijing Municipal Science and Technology Commission ; Peking University ; National Natural Science Foundation of China
WOS记录号: WOS:000328693300007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66660
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Coll Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Ctr Reprod Med, Beijing 100871, Peoples R China
3.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
4.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
5.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
6.Peking Tsinghua Ctr Life Sci, Beijing 100084, Peoples R China
7.Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China

Recommended Citation:
Hou, Yu,Fan, Wei,Yan, Liying,et al. Genome Analyses of Single Human Oocytes[J]. CELL,2013,155(7):1492-1506.
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