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IR@PKUHSC  > 北京大学药学院  > 化学生物学系  > 期刊论文
学科主题: 药学
题名:
Nature of cerium(III)- and lanthanum(III)-induced aggregation of human erythrocyte membrane proteins
作者: Du, XL; Zhang, TL; Li, RC; Wang, K
关键词: membrane proteins ; human erythrocytes ; aggregation ; cerium(III) ; lanthanum(III)
刊名: JOURNAL OF INORGANIC BIOCHEMISTRY
发表日期: 2001-03-01
卷: 84, 期:1-2, 页:67-75
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: DYNAMIC LIGHT-SCATTERING ; INFRARED-SPECTROSCOPY ; SECONDARY STRUCTURE ; LIPID-PEROXIDATION ; BINDING ; CYTOSKELETON ; SPECTRA ; IONS ; HEAD
英文摘要:

To clarify the nature of the aggregation of membrane proteins (MP) induced by lanthanide cations (Lns), the interaction of cerium(III) (Ce3+) and lanthanum(III)(La3+) with erythrocyte membrane proteins was studied by means of SDS-PAGE, light scattering measurement, fluorescence, CD and FTIR spectra. The results showed that Ce3+ and La3+ induce protein aggregation not only by Lns non-covalent binding and cross-linking, but also by oxidative cross-linking through disulfide bond formation. As demonstrated by intrinsic fluorescence, CD and FTIR spectra studies, the aggregation was accompanied by the conformation changes with tryptophane residues exposing to more hydrophobic environment and the decreasing alpha -helix and beta -sheet contents. By stopped-flow studies, protein aggregation was shown to be a slow change, which is initiated by rapid Lns binding and then followed by subsequent conformational changes. (C) 2001 Elsevier Science B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000168175300009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66661
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China

Recommended Citation:
Du, XL,Zhang, TL,Li, RC,et al. Nature of cerium(III)- and lanthanum(III)-induced aggregation of human erythrocyte membrane proteins[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2001,84(1-2):67-75.
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