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Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates
Ma, Chunying1; Li, Chao1; Luan, Xingrong1; Zhang, Jin1; Qiao, Renzhong1,2; Zhao, Yufen3
刊名RAPID COMMUNICATIONS IN MASS SPECTROMETRY
2014-03-30
DOI10.1002/rcm.6822
28期:6页:645-652
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
关键词[WOS]PEPTIDE IONS ADJACENT ; GAS-PHASE CLEAVAGE ; PROTONATED PEPTIDES ; ACID-RESIDUES ; ASPARTIC-ACID ; CHEMISTRY ; PATHWAYS ; BOND
英文摘要

RATIONALEDifferentiation and structural characterization of Asp/cyclen conjugates by electrospray ionization tandem mass spectrometry (ESI-MSn) are significantly important for their biomedical application. Hence, the present study is conducted.

METHODSThe fragmentations of Asp/cyclen conjugates generated by positive ion mode electrospray ionization were examined here by low-energy collision-induced dissociation (CID). ESI-MSn spectra of cyclen were acquired to confirm cyclen contraction products derived from the studied compounds. The fragments derived from the Asp/cyclen conjugates were proved by deuterium-exchange experiments.

RESULTSAsp/cyclen conjugates displayed characteristic dissociation pathways, including cleavages of amide bonds, loss of NH3 and cyclen contraction pathways. It was observed that cleavages of C-terminal amide bonds generated b(2) and b(2)+H2O ions from the protonated CyclenAspAspAsp and a b(1)+H2O ion from the protonated CyclenAspAsp. In addition, various cyclen contraction products were also observed.

CONCLUSIONSIn ESI-MSn spectra of studied compounds, fragments of b(n-1)+H2O or cyclic anhydride were generated due to facile mobilization of C-terminal or side-chain COOH protons. In addition, the cyclen contraction products were detected. These results might provide sufficient information for the identification of Asp/cyclen conjugates by mass spectrometry. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000330976100009
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66665
专题北京大学药学院
北京大学口腔医学院_材料检测中心
作者单位1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China
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GB/T 7714
Ma, Chunying,Li, Chao,Luan, Xingrong,et al. Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2014,28(6):645-652.
APA Ma, Chunying,Li, Chao,Luan, Xingrong,Zhang, Jin,Qiao, Renzhong,&Zhao, Yufen.(2014).Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,28(6),645-652.
MLA Ma, Chunying,et al."Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 28.6(2014):645-652.
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