北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates
作者: Ma, Chunying1; Li, Chao1; Luan, Xingrong1; Zhang, Jin1; Qiao, Renzhong1,2; Zhao, Yufen3
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2014-03-30
DOI: 10.1002/rcm.6822
卷: 28, 期:6, 页:645-652
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
关键词[WOS]: PEPTIDE IONS ADJACENT ; GAS-PHASE CLEAVAGE ; PROTONATED PEPTIDES ; ACID-RESIDUES ; ASPARTIC-ACID ; CHEMISTRY ; PATHWAYS ; BOND
英文摘要:

RATIONALEDifferentiation and structural characterization of Asp/cyclen conjugates by electrospray ionization tandem mass spectrometry (ESI-MSn) are significantly important for their biomedical application. Hence, the present study is conducted.

METHODSThe fragmentations of Asp/cyclen conjugates generated by positive ion mode electrospray ionization were examined here by low-energy collision-induced dissociation (CID). ESI-MSn spectra of cyclen were acquired to confirm cyclen contraction products derived from the studied compounds. The fragments derived from the Asp/cyclen conjugates were proved by deuterium-exchange experiments.

RESULTSAsp/cyclen conjugates displayed characteristic dissociation pathways, including cleavages of amide bonds, loss of NH3 and cyclen contraction pathways. It was observed that cleavages of C-terminal amide bonds generated b(2) and b(2)+H2O ions from the protonated CyclenAspAspAsp and a b(1)+H2O ion from the protonated CyclenAspAsp. In addition, various cyclen contraction products were also observed.

CONCLUSIONSIn ESI-MSn spectra of studied compounds, fragments of b(n-1)+H2O or cyclic anhydride were generated due to facile mobilization of C-terminal or side-chain COOH protons. In addition, the cyclen contraction products were detected. These results might provide sufficient information for the identification of Asp/cyclen conjugates by mass spectrometry. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 21232005 ; 21372024 ; 21202005 ; 21172016
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000330976100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66665
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China

Recommended Citation:
Ma, Chunying,Li, Chao,Luan, Xingrong,et al. Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2014,28(6):645-652.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ma, Chunying]'s Articles
[Li, Chao]'s Articles
[Luan, Xingrong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ma, Chunying]‘s Articles
[Li, Chao]‘s Articles
[Luan, Xingrong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace