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学科主题: 药学
题名:
Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates
作者: Ma, Chunying1; Li, Chao1; Luan, Xingrong1; Zhang, Jin1; Qiao, Renzhong1,2; Zhao, Yufen3
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2014-03-30
DOI: 10.1002/rcm.6822
卷: 28, 期:6, 页:645-652
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
关键词[WOS]: PEPTIDE IONS ADJACENT ; GAS-PHASE CLEAVAGE ; PROTONATED PEPTIDES ; ACID-RESIDUES ; ASPARTIC-ACID ; CHEMISTRY ; PATHWAYS ; BOND
英文摘要:

RATIONALEDifferentiation and structural characterization of Asp/cyclen conjugates by electrospray ionization tandem mass spectrometry (ESI-MSn) are significantly important for their biomedical application. Hence, the present study is conducted.

METHODSThe fragmentations of Asp/cyclen conjugates generated by positive ion mode electrospray ionization were examined here by low-energy collision-induced dissociation (CID). ESI-MSn spectra of cyclen were acquired to confirm cyclen contraction products derived from the studied compounds. The fragments derived from the Asp/cyclen conjugates were proved by deuterium-exchange experiments.

RESULTSAsp/cyclen conjugates displayed characteristic dissociation pathways, including cleavages of amide bonds, loss of NH3 and cyclen contraction pathways. It was observed that cleavages of C-terminal amide bonds generated b(2) and b(2)+H2O ions from the protonated CyclenAspAspAsp and a b(1)+H2O ion from the protonated CyclenAspAsp. In addition, various cyclen contraction products were also observed.

CONCLUSIONSIn ESI-MSn spectra of studied compounds, fragments of b(n-1)+H2O or cyclic anhydride were generated due to facile mobilization of C-terminal or side-chain COOH protons. In addition, the cyclen contraction products were detected. These results might provide sufficient information for the identification of Asp/cyclen conjugates by mass spectrometry. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 21232005 ; 21372024 ; 21202005 ; 21172016
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000330976100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66665
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China

Recommended Citation:
Ma, Chunying,Li, Chao,Luan, Xingrong,et al. Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2014,28(6):645-652.
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