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学科主题: 临床医学
题名:
Biodegradable Poly(lactic-co-glycolic acid) Maxpol-T/S as Novel Scaffold for Adipose Derived Stem Cells and Fibroblast Growth In Vitro
作者: Jin, Zhe1; Gong, Yan-Qing1; Qin, Xin-Cheng1; Zhang, Jian1; Zhu, Yi-Chen; Lin, Gui-ting2; Lue, Tom F.2; Xin, Zhong-Cheng1
关键词: Adipose-derived stem cells ; fibroblasts ; GFP ; PLGA mesh ; cell adhesion ; tissue engineering
刊名: TISSUE ENGINEERING AND REGENERATIVE MEDICINE
发表日期: 2008-12-01
卷: 5, 期:4-6, 页:869-876
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Engineering, Biomedical
研究领域[WOS]: Cell Biology ; Engineering
关键词[WOS]: VENOUS PATCH GRAFT ; PEYRONIES-DISEASE ; DIFFERENTIATION ; REGENERATION
英文摘要:

To explore the ability of MaxPol-T/S, a novelbiodegradable poly-lactic-co-glycolic acid(PLGA) scaffold, in providing a cell-matrix interaction interface for cell growth, we generated GFP positive adipose derived stem cell(ADSC(GFP+)) and fibroblasts(FC(GFP+)) for investigating the cellular growth on this biomaterial. The MaxPol-T/S was produced through salt-leaching/particulate-leaching technology for tissue engineering, which provides a modified surface for the best cellular attachment sites and nutrient supply and waste exchange conditions. The morphological features of MaxPol-T/S were studied with a scanning electron microscope and the ADSC(GFP+) and FC(GFP+) were confirmed by auto-fluorescence microscopy and flow cytometry assay. Being seeded onto the MaxPol-T/S in vitro, the growth and morphology of ADSC(GFP+) and FC(GFP+) were further verified by auto-fluorescence microscopy and MMT test. The ADSCs and fibroblasts, expressed strong GFP signals in the cytoplasm and nucleus, adhered and proliferated on the surface of scaffold MaxPol-T/S. Both cell lines survived on the scaffold more than 21 days in vitro and formed three-dimensional colonies on the surface of the MaxPol-T. In conclusion, MaxPol-T/S is a novel PLGA scaffold for ADSC(GFP+) and FC(GFP+) and implies a promising technique for tissue engineering.

语种: 英语
WOS记录号: WOS:000261380700045
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66669
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Peking Univ, Androl Ctr, Hosp 1, Beijing 100034, Peoples R China
2.Univ Calif San Francisco, Dept Urol, Sch Med, San Francisco, CA 94143 USA

Recommended Citation:
Jin, Zhe,Gong, Yan-Qing,Qin, Xin-Cheng,et al. Biodegradable Poly(lactic-co-glycolic acid) Maxpol-T/S as Novel Scaffold for Adipose Derived Stem Cells and Fibroblast Growth In Vitro[J]. TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2008,5(4-6):869-876.
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