IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation
Cui, Guanghui; Qi, Zhengyu; Guo, Xin; Qin, Jie; Gui, Yaoting; Cai, Zhiming1
关键词Bone marrow Mesenchymal progenitor cell Embryonic stem cell Primordial germ cell Retinoic acid Differentiation
刊名CELL BIOLOGY INTERNATIONAL
2009-03-01
DOI10.1016/j.cellbi.2009.01.008
33期:3页:434-441
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
资助者Hong Kong Creative Circle Plan [2008] 121 ; China Post doctor fund ; Hong Kong Creative Circle Plan [2008] 121 ; China Post doctor fund
研究领域[WOS]Cell Biology
关键词[WOS]STEM-CELLS ; ADULT MARROW ; DERIVATION ; ORIGIN
英文摘要

Mouse embryonic fibroblasts (MEFs) have been used as feeder cells to support the growth of mouse embryonic stem cell (mESC) and primordial germ cells (PGC) in culture for many years. However, MEF preparation is a complex and tedious task. Recently, there are reports indicating that the microenvironment provided by bone marrow stromal cells could support the survival of embryonic-like stem cells in bone marrow. In this report, rat bone marrow derived mesenchymal progenitor cells (MPC) were used as feeder cells to culture mouse Oct4-GFP ES cell and ES cell derived germ cells. FACS results show that similar to MEF, rat MPC could efficiently support growth of the mouse Oct4-GFP ES cell line in culture (MPC 85.5 +/- 5.1% vs MEF 84.1 +/- 6.2%). ES cells could be subcultured for > 15 passages without losing morphological characteristics. The cultured cells expressed stem cell marker alkaline phosphatase, Oct4, Sox2, and SSEA-1. Furthermore, rat MPC cells were able to support survival of germ cells isolated from mouse Oct4-GFP ES cell formed embryoid bodies (EB). After induction by retinoic acid for 7 days, some isolated cells differentiated to spermatogonial stem-like cells, expressing Mvh, Stra-8, Hsp90-alpha, integrin beta 1 and alpha 6. Compared with traditional MEF culture systems, the rat MPC culture system is effective in supporting ES cell growth and is easy to prepare. (C) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

语种英语
所属项目编号20080430012
资助者Hong Kong Creative Circle Plan [2008] 121 ; China Post doctor fund ; Hong Kong Creative Circle Plan [2008] 121 ; China Post doctor fund
WOS记录号WOS:000263890200024
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66679
Collection北京大学深圳医院
作者单位1.Peking Univ, Med Ctr, Shenzhen Hosp, Lab Male Reprod, Beijing 518036, Guangdong, Peoples R China
2.Hong Kong Univ Sci & Technol, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Cui, Guanghui,Qi, Zhengyu,Guo, Xin,et al. Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation[J]. CELL BIOLOGY INTERNATIONAL,2009,33(3):434-441.
APA Cui, Guanghui,Qi, Zhengyu,Guo, Xin,Qin, Jie,Gui, Yaoting,&Cai, Zhiming.(2009).Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation.CELL BIOLOGY INTERNATIONAL,33(3),434-441.
MLA Cui, Guanghui,et al."Rat bone marrow derived mesenchymal progenitor cells support mouse ES cell growth and germ-like cell differentiation".CELL BIOLOGY INTERNATIONAL 33.3(2009):434-441.
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