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学科主题: 药学
题名:
Analysis of breast cancer-associated glycosphingolipids using electrospray ionization-linear ion trap quadrupole mass spectrometry
作者: Zhu, Tingting1; Xu, Longjiang2; Xu, Xiukun1; Wang, Zheng1; Zhu, Jian1; Xie, Qing1; Zhang, Biyan3; Wang, Yanping1; Ju, Linling1; He, Yun1; Ye, Xinshan5; Zhou, Dapeng4; Li, Yunsen1
关键词: Glycosphingolipids ; Mass spectrometry ; Breast cancer ; Fucosyl-lactoceramide ; Fucosyltransferases
刊名: CARBOHYDRATE RESEARCH
发表日期: 2015-01-30
DOI: 10.1016/j.carres.2014.10.006
卷: 402, 页:189-199
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Applied ; Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: MONOCLONAL-ANTIBODY MBR1 ; SOLUBLE E-SELECTIN ; MULTIDRUG-RESISTANCE ; EPITHELIAL-CELLS ; GANGLIOSIDE GD2 ; HUMAN-MELANOMA ; STEM-CELLS ; GLOBO-H ; ANTIGEN ; EXPRESSION
英文摘要:

Abnormal glycosphingolipids (GSLs) are expressed in many human tumors. These tumor-associated GSLs may have important roles in tumor progression. However, they are hard to be detected because of their low concentration and the limited availability of antibodies and lectins that recognize them. Thus, mass spectrometry is an effective method to analyze GSLs with high sensitivity. Here, we use electrospray ionization-linear ion trap quadrupole mass spectrometry (ESI-LTQ-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) to determine the structure of a certain GSL in human breast cancer tissue. To obtain the breast cancer-associated GSLs, we applied relative abundance contrast of GSLs and signal-to-noise ratio (SNR) analyses. We also used alpha 1,2 fucosidase and fucosyltransferases as tools in the structural analysis. Based on this analysis, we identified the ion with m/z 1184 molecular ion as fucosyl-lactoceramide (Fuc-LacCer) with a C16 fatty acid ceramide. Quantitative analysis of GSLs revealed both Fuc-LacCer and Globo-H increased in breast cancer tissues. However, these two breast cancer-associated GSLs had different roles. The results of SNR analysis suggested the abnormal Fuc-LacCer is specific to breast cancer. The GSL profiling of breast cancer cells showed fucosyltransferase 1 contributed to the biosynthesis of Globo-H and Fuc-LacCer. In conclusion, MS analysis identified an accumulation of Fuc-LacCer in breast cancer tissue. Our findings provide GSL profiles of human breast cancer and develop an MS method for the study of cancer-associated GSLs. (C) 2014 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 31000370 ; BK2011288 ; 2012ZX09401-014 ; 2012ZX09502001-001 ; 2012CB822100 ; IRT1075
项目资助者: National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Province ; National Science and Technology key projects during the Twelfth Five-Year Plan Period of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Jiangsu Provincial Innovative Research Team
WOS记录号: WOS:000346825800024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66684
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Soochow Univ, Jiangsu Lab Infect Immun, Inst Biol & Med Sci, Lab Cellular & Mol Tumor Immunol, Suzhou 215123, Peoples R China
2.Soochow Univ, Affiliated Hosp Soochow 2, Suzhou 215004, Peoples R China
3.Shanghai Univ Tradit Chinese Med, Sch Pharm, Dept Pharmaceut, Shanghai 201210, Peoples R China
4.Univ Texas MD Anderson Canc Ctr, Dept Melanoma Med Oncol, Houston, TX 77030 USA
5.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

Recommended Citation:
Zhu, Tingting,Xu, Longjiang,Xu, Xiukun,et al. Analysis of breast cancer-associated glycosphingolipids using electrospray ionization-linear ion trap quadrupole mass spectrometry[J]. CARBOHYDRATE RESEARCH,2015,402:189-199.
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