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学科主题临床医学
CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis
Chen, Hao1,2; Fok, Kin Lam1; Yu, Sidney1; Jiang, Jianli3,4; Chen, Zhinan3,4; Gui, Yaoting2; Cai, Zhiming2; Chan, Hsiao Chang1
关键词spermatogenesis CD147 MMP-2
刊名MOLECULAR HUMAN REPRODUCTION
2011-07-01
DOI10.1093/molehr/gar013
17期:7页:405-414
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology ; Obstetrics & Gynecology ; Reproductive Biology
资助者National Natural Science Foundation of China ; National 973 project ; Li Ka Shing Institute of Health Sciences ; Chinese University of Hong Kong ; National Natural Science Foundation of China ; National 973 project ; Li Ka Shing Institute of Health Sciences ; Chinese University of Hong Kong
研究领域[WOS]Developmental Biology ; Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]EXTRACELLULAR-MATRIX ; IMMUNOGLOBULIN-SUPERFAMILY ; TISSUE INHIBITOR ; JUNCTION DYNAMICS ; EXPRESSION ; INDUCER ; EMMPRIN ; MMP-2 ; CARCINOMA ; BASIGIN
英文摘要

Spermatogenesis is a highly programmed process that requires the degradation of the extracellular matrix and the remodeling of tight junctions (TJ) to facilitate differentiating germ cell migration. Matrix metalloproteinases (MMPs) are essential in regulating Sertoli cell TJ in the testis. CD147 is known to stimulate the production of MMPs in tumor metastasis and its knockout mice are infertile. However, the functional relationship between CD147 and MMPs in spermatogenesis has not been investigated. In the present study, we examined the expression profile of CD147 and MMPs during mouse testicular development by RT-PCR, western blot and immunofluorescence staining. We also examined CD147 involvement in the production of MMP-2 and the migration of germ cells (GC-1 and GC-2 cells) using CD147 antibody or synthetic microRNA mimics-mediated knockdown. The results showed that CD147 was present at all stages of testicular development from 7 to 56 days post-partum (dpp). CD147 expression was found to increase after 21 days from moderate levels in 7 and 14 days. Of the eight MMPs studied, MMP-2, MMP-7, MMP-9 and MMP-23 were detected to have changes in expression during testicular development, with MMP-2 showing the largest change. CD147 and MMP-2 were co-localized in spermatogonia, spermatocytes and round spermatids in mouse testis, while in human testis, they were co-localized in spermatocytes and round spermatids. MMP-2 expression and migration of GC-1 and GC-2 cells were reduced by interfering with CD147 expression and function in vitro. These data suggest that CD147 regulates migration of spermatogonia and spermatocytes via induction of MMP-2 production during spermatogenesis.

语种英语
所属项目编号30971636 ; 30972992 ; 2006CB504002
资助者National Natural Science Foundation of China ; National 973 project ; Li Ka Shing Institute of Health Sciences ; Chinese University of Hong Kong ; National Natural Science Foundation of China ; National 973 project ; Li Ka Shing Institute of Health Sciences ; Chinese University of Hong Kong
WOS记录号WOS:000291755400002
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66693
专题北京大学深圳医院
作者单位1.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Epithelial Cell Biol Res Ctr, Shatin, Hong Kong, Peoples R China
2.Peking Univ Shenzhen Hosp, Shenzhen PKU HKUST Med Ctr, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen, Peoples R China
3.Fourth Mil Med Univ, Cell Engn Res Ctr, Xian, Shanxi, Peoples R China
4.Fourth Mil Med Univ, Dept Cell Biol, State Key Lab Canc Biol, Xian, Shanxi, Peoples R China
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Chen, Hao,Fok, Kin Lam,Yu, Sidney,et al. CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis[J]. MOLECULAR HUMAN REPRODUCTION,2011,17(7):405-414.
APA Chen, Hao.,Fok, Kin Lam.,Yu, Sidney.,Jiang, Jianli.,Chen, Zhinan.,...&Chan, Hsiao Chang.(2011).CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis.MOLECULAR HUMAN REPRODUCTION,17(7),405-414.
MLA Chen, Hao,et al."CD147 is required for matrix metalloproteinases-2 production and germ cell migration during spermatogenesis".MOLECULAR HUMAN REPRODUCTION 17.7(2011):405-414.
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