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学科主题: 药学
题名:
Identification of Multiple Constituents in Chinese Medicinal Prescription Shensong Yangxin Capsule by Ultra-Fast Liquid Chromatography Combined with Quadrupole Time-of-Flight Mass Spectrometry
作者: Liu, Minyan1,2; Zhao, Shaohua2; Wang, Yufeng2; Liu, Ting3; Li, Song2; Wang, Hongtao2; Tu, Pengfei1
刊名: JOURNAL OF CHROMATOGRAPHIC SCIENCE
发表日期: 2015-02-01
DOI: 10.1093/chromsci/bmu047
卷: 53, 期:2, 页:240-252
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: EXTRACT ; GLYCOSIDES ; CHINENSIS ; LIGNANS ; MS/MS ; HPLC
英文摘要:

A practical method using ultra-fast liquid chromatography in tandem with quadrupole time-of-flight mass spectrometry combined with dynamic background subtraction technology was developed for the rapid separation and identification of the complicated constituents in the Shensong Yangxin capsule (SSYX). The chromatographic separation was performed on a C18 column (2.1 x 100 mm, 2.6 mu m) with a gradient elution program using methanol and 0.1% formic acid aqueous solution as the mobile phase at a flow rate of 0.4 mL min(-1). Accurate mass measurements of the molecular ions in the full scan and the characteristic fragment ions triggered by information-dependent acquisition provided reliable identification criteria. Thus, 99 compounds, including saponins, phenolic acids, tanshinones, lignans, terpenoids, alkaloids and flavonoids, were unambiguously or tentatively identified in 40 min by comparing their retention times and accurate mass measurements for each molecular ion and its subsequent fragment ions with those of authentic standards or literature data. Simultaneously, all the compounds were further assigned to the individual raw materials. In conclusion, these results will provide a basis for quality control and further study of SSYX, and the proposed technique based on high-resolution mass spectrometry would be expected to be adaptable to the analysis of complicated constituents in various complex matrices.

语种: 英语
所属项目编号: 2012CB518606 ; 2011ZX09201-201-28
项目资助者: National Key Basic Research Program of China (973 Program) ; National Great Scientific and Technological Project of China
WOS记录号: WOS:000352281800007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66701
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Shijiazhuang Yiling Pharmaceut Co Ltd, Shijiazhuang 050035, Peoples R China
3.Shanghai AB Sciex Analyt Instrument Trading Co Lt, Shanghai 200040, Peoples R China

Recommended Citation:
Liu, Minyan,Zhao, Shaohua,Wang, Yufeng,et al. Identification of Multiple Constituents in Chinese Medicinal Prescription Shensong Yangxin Capsule by Ultra-Fast Liquid Chromatography Combined with Quadrupole Time-of-Flight Mass Spectrometry[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(2):240-252.
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