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学科主题: 基础医学
题名:
Biosafety level 3 laboratory for autopsies of patients with severe acute respiratory syndrome: Principles, practices, and prospects
作者: Li, L; Gu, J; Shi, XC; Gong, EC; Li, XW; Shao, HQ; Shi, XY; Jiang, HJ; Gao, XQ; Cheng, DY; Guo, LZ; Wang, H; Shi, XH; Wang, PZ; Zhang, QY; Shen, B
刊名: CLINICAL INFECTIOUS DISEASES
发表日期: 2005-09-15
卷: 41, 期:6, 页:815-821
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Infectious Diseases ; Microbiology
研究领域[WOS]: Immunology ; Infectious Diseases ; Microbiology
关键词[WOS]: SYNDROME SARS ; INFECTION
英文摘要:

Background. During the outbreak of the emergent severe acute respiratory syndrome (SARS) infection, > 30% of the similar to 8000 infected persons were health care workers. The highly infectious nature of SARS coronavirus (SARS-CoV) compelled our pathologists to consider biosafety issues in the autopsy room and for tissue processing procedures.

Methods. A specially designed biosafety level 3 (BSL-3) autopsy laboratory was constructed and divided into a clean area, a semicontaminated area, a contaminated area, and 2 buffer zones. High-efficiency particulate air filters were placed in the air supply and exhaust systems. Laminar air flow was from the clean areas to the less clean areas. The negative pressures of the contaminated, semicontaminated, and clean areas were approximately -50 pa, -25 pa, and -5 pa, respectively. Personal protective equipment, including gas mask, impermeable protective clothing, and 3 layers of gloves worn during autopsies; the equipment was decontaminated before it was allowed to exit the facility. Strict BSL-3 practices were followed.

Results. When a given concentration of particulate sarin simulant was introduced into the contaminated area, it could not be detected in either the semicontaminated area or clean area, and particles > 0.3 mu m in size were not detected in the exhaust air. A total of 16 complete postmortem examinations for probable and suspected SARS were performed during a 2-month period. Of these, 7 reported confirmed cases of SARS. None of the 23 pathologists and technicians who participated in these autopsies was infected with SARS-CoV.

Conclusions. Our experience suggests that BSL-3 laboratory operating principles should be among the special requirements for performing autopsies of contaminated bodies and that they can safeguard the clinicians and the environment involved in these procedures.

语种: 英语
WOS记录号: WOS:000231313800008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66718
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Res Inst Chem Def, Beijing, Peoples R China
2.Beijing Ditan Hosp, Beijing, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Pathol, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Li, L,Gu, J,Shi, XC,et al. Biosafety level 3 laboratory for autopsies of patients with severe acute respiratory syndrome: Principles, practices, and prospects[J]. CLINICAL INFECTIOUS DISEASES,2005,41(6):815-821.
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