北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科  > 期刊论文
学科主题: 临床医学
题名:
A novel system of artificial antigen-presenting cells efficiently stimulates Flu peptide-specific cytotoxic T cells in vitro
作者: Han, Hui1; Peng, Ji-Run1; Chen, Peng-Cheng1; Gong, Lei1; Qiao, Shi-Shi2; Wang, Wen-Zhen1; Cui, Zhu-Qingqing1; Yu, Xin1; Wei, Yu-Hua1; Leng, Xi-Sheng1
关键词: Artificial antigen-presenting cells ; Adoptive immunotherapy ; Controlled release ; Cytotoxic T lymphocytes ; Interleukin-2
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2011-08-05
DOI: 10.1016/j.bbrc.2011.06.164
卷: 411, 期:3, 页:530-535
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: METASTATIC MELANOMA ; ADOPTIVE TRANSFER ; DENDRITIC CELLS ; NK CELLS ; VIVO ; IMMUNOTHERAPY ; EXPANSION ; THERAPY ; INDUCTION ; ANTIBODY
英文摘要:

Therapeutic numbers of antigen-specific cytotoxic T lymphocytes (CTLs) are key effectors in successful adoptive immunotherapy. However, efficient and reproducible methods to meet the qualification remain poor. To address this issue, we designed the artificial antigen-presenting cell (aAPC) system based on poly(lactic-co-glycolic acid) (PLGA). A modified emulsion method was used for the preparation of PLGA particles encapsulating interleukin-2 (IL-2). Biotinylated molecular ligands for recognition and co-stimulation of T cells were attached to the particle surface through the binding of avidin-biotin. These formed the aAPC system. The function of aAPCs in the proliferation of specific CTLs against human Flu antigen was detected by enzyme-linked immunospot assay (ELISPOT) and MIT staining methods. Finally, we successfully prepared this suitable aAPC system. The results show that IL-2 is released from aAPCs in a sustained manner over 30 days. This dramatically improves the stimulatory capacity of this system as compared to the effect of exogenous addition of cytokine. In addition, our aAPCs promote the proliferation of Flu antigen-specific CTLs more effectively than the autologous cellular APCs. Here, this aAPC platform is proved to be suitable for expansion of human antigen-specific T cells. (C) 2011 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30772119 ; RDB 2009-15
项目资助者: National Natural Science Foundation of China ; Peking University People&prime ; s Hospital
WOS记录号: WOS:000294309800011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66724
Appears in Collections:北京大学第二临床医学院_肝胆外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Dept Hepatobiliary Surg, Beijing 100044, Peoples R China
2.Zhengzhou Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Zhengzhou 450052, Peoples R China

Recommended Citation:
Han, Hui,Peng, Ji-Run,Chen, Peng-Cheng,et al. A novel system of artificial antigen-presenting cells efficiently stimulates Flu peptide-specific cytotoxic T cells in vitro[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,411(3):530-535.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Han, Hui]'s Articles
[Peng, Ji-Run]'s Articles
[Chen, Peng-Cheng]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Han, Hui]‘s Articles
[Peng, Ji-Run]‘s Articles
[Chen, Peng-Cheng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace