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A novel system of artificial antigen-presenting cells efficiently stimulates Flu peptide-specific cytotoxic T cells in vitro
Han, Hui1; Peng, Ji-Run1; Chen, Peng-Cheng1; Gong, Lei1; Qiao, Shi-Shi2; Wang, Wen-Zhen1; Cui, Zhu-Qingqing1; Yu, Xin1; Wei, Yu-Hua1; Leng, Xi-Sheng1
关键词Artificial antigen-presenting cells Adoptive immunotherapy Controlled release Cytotoxic T lymphocytes Interleukin-2
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2011-08-05
DOI10.1016/j.bbrc.2011.06.164
411期:3页:530-535
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]METASTATIC MELANOMA ; ADOPTIVE TRANSFER ; DENDRITIC CELLS ; NK CELLS ; VIVO ; IMMUNOTHERAPY ; EXPANSION ; THERAPY ; INDUCTION ; ANTIBODY
英文摘要

Therapeutic numbers of antigen-specific cytotoxic T lymphocytes (CTLs) are key effectors in successful adoptive immunotherapy. However, efficient and reproducible methods to meet the qualification remain poor. To address this issue, we designed the artificial antigen-presenting cell (aAPC) system based on poly(lactic-co-glycolic acid) (PLGA). A modified emulsion method was used for the preparation of PLGA particles encapsulating interleukin-2 (IL-2). Biotinylated molecular ligands for recognition and co-stimulation of T cells were attached to the particle surface through the binding of avidin-biotin. These formed the aAPC system. The function of aAPCs in the proliferation of specific CTLs against human Flu antigen was detected by enzyme-linked immunospot assay (ELISPOT) and MIT staining methods. Finally, we successfully prepared this suitable aAPC system. The results show that IL-2 is released from aAPCs in a sustained manner over 30 days. This dramatically improves the stimulatory capacity of this system as compared to the effect of exogenous addition of cytokine. In addition, our aAPCs promote the proliferation of Flu antigen-specific CTLs more effectively than the autologous cellular APCs. Here, this aAPC platform is proved to be suitable for expansion of human antigen-specific T cells. (C) 2011 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000294309800011
项目编号30772119 ; RDB 2009-15
资助机构National Natural Science Foundation of China ; Peking University People&prime ; s Hospital
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66724
专题北京大学第二临床医学院_肝胆外科
北京大学精神卫生研究所_精神科
作者单位1.Peking Univ, Peoples Hosp, Dept Hepatobiliary Surg, Beijing 100044, Peoples R China
2.Zhengzhou Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Zhengzhou 450052, Peoples R China
推荐引用方式
GB/T 7714
Han, Hui,Peng, Ji-Run,Chen, Peng-Cheng,et al. A novel system of artificial antigen-presenting cells efficiently stimulates Flu peptide-specific cytotoxic T cells in vitro[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,411(3):530-535.
APA Han, Hui.,Peng, Ji-Run.,Chen, Peng-Cheng.,Gong, Lei.,Qiao, Shi-Shi.,...&Leng, Xi-Sheng.(2011).A novel system of artificial antigen-presenting cells efficiently stimulates Flu peptide-specific cytotoxic T cells in vitro.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,411(3),530-535.
MLA Han, Hui,et al."A novel system of artificial antigen-presenting cells efficiently stimulates Flu peptide-specific cytotoxic T cells in vitro".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 411.3(2011):530-535.
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