|Chemical Synthesis of Bacteriophage G4|
|Yang, Ruilin1,2,3; Han, Yonghua1; Ye, Yiwang1; Liu, Yuchen1,3; Jiang, Zhimao1; Gui, Yaoting1; Cai, Zhiming1,4|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||PHIX-LIKE PHAGES ; NUCLEOTIDE-SEQUENCE ; GENOME ; VIRUS ; SUSCEPTIBILITY ; PATHOGENS ; BIOLOGY ; GENE|
Background: Due to recent leaps forward in DNA synthesis and sequencing technology, DNA manipulation has been extended to the level of whole-genome synthesis. Bacteriophages occupy a special niche in the micro-organic ecosystem and have potential as a tool for therapeutic agent. The purpose of this study was to carry out chemical synthesis of the bacteriophage G4 and the study of its infectivity.
Methodology/Principal Findings: Full-sized genomes of bacteriophage G4 molecules were completed from short overlapping synthetic oligonucleotides by direct assembly polymerase chain reaction and ligase chain reaction followed by fusion polymerase chain reaction with flanking primers. Three novel restriction endonuclease sites were introduced to distinguish the synthetic G4 from the wild type. G4 particles were recovered after electroporation into Escherichia coli and were efficient enough to infect another strain. The phage was validated by electron microscope. Specific polymerase chain reaction assay and restriction analyses of the plaques verified the accuracy of the chemical synthetic genomes.
Conclusions: Our results showed that the bacteriophage G4 obtained is synthetic rather than a wild type. Our study demonstrated that a phage can be synthesized and manipulated genetically according to the sequences, and can be efficient enough to infect the Escherichia coli, showing the potential use of synthetic biology in medical application.
|项目编号||CXB200903090055A ; 200800010106|
|资助机构||Promotion Program for Shenzhen Key Laboratory, Shenzhen, China ; Ph.D. Programs Foundation of Ministry of Education of China|
|作者单位||1.Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol, Shenzhen Hosp,Shenzhen PKU HKUST Med Ctr, Shenzhen, Peoples R China|
2.Shenzhen Univ, Urogenital Inst, Shenzhen Peoples Hosp 2, Shenzhen, Peoples R China
3.Guangzhou Panyu Cent Hosp, Guangzhou, Guangdong, Peoples R China
4.Shantou Univ Med Coll, Shantou, Peoples R China
|Yang, Ruilin,Han, Yonghua,Ye, Yiwang,et al. Chemical Synthesis of Bacteriophage G4[J]. PLOS ONE,2011,6(11).|
|APA||Yang, Ruilin.,Han, Yonghua.,Ye, Yiwang.,Liu, Yuchen.,Jiang, Zhimao.,...&Cai, Zhiming.(2011).Chemical Synthesis of Bacteriophage G4.PLOS ONE,6(11).|
|MLA||Yang, Ruilin,et al."Chemical Synthesis of Bacteriophage G4".PLOS ONE 6.11(2011).|