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学科主题: 临床医学
题名:
Chemical Synthesis of Bacteriophage G4
作者: Yang, Ruilin1,2,3; Han, Yonghua1; Ye, Yiwang1; Liu, Yuchen1,3; Jiang, Zhimao1; Gui, Yaoting1; Cai, Zhiming1,4
刊名: PLOS ONE
发表日期: 2011-11-16
DOI: 10.1371/journal.pone.0027062
卷: 6, 期:11
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: PHIX-LIKE PHAGES ; NUCLEOTIDE-SEQUENCE ; GENOME ; VIRUS ; SUSCEPTIBILITY ; PATHOGENS ; BIOLOGY ; GENE
英文摘要:

Background: Due to recent leaps forward in DNA synthesis and sequencing technology, DNA manipulation has been extended to the level of whole-genome synthesis. Bacteriophages occupy a special niche in the micro-organic ecosystem and have potential as a tool for therapeutic agent. The purpose of this study was to carry out chemical synthesis of the bacteriophage G4 and the study of its infectivity.

Methodology/Principal Findings: Full-sized genomes of bacteriophage G4 molecules were completed from short overlapping synthetic oligonucleotides by direct assembly polymerase chain reaction and ligase chain reaction followed by fusion polymerase chain reaction with flanking primers. Three novel restriction endonuclease sites were introduced to distinguish the synthetic G4 from the wild type. G4 particles were recovered after electroporation into Escherichia coli and were efficient enough to infect another strain. The phage was validated by electron microscope. Specific polymerase chain reaction assay and restriction analyses of the plaques verified the accuracy of the chemical synthetic genomes.

Conclusions: Our results showed that the bacteriophage G4 obtained is synthetic rather than a wild type. Our study demonstrated that a phage can be synthesized and manipulated genetically according to the sequences, and can be efficient enough to infect the Escherichia coli, showing the potential use of synthetic biology in medical application.

语种: 英语
所属项目编号: CXB200903090055A ; 200800010106
项目资助者: Promotion Program for Shenzhen Key Laboratory, Shenzhen, China ; Ph.D. Programs Foundation of Ministry of Education of China
WOS记录号: WOS:000297555400018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66757
Appears in Collections:北京大学深圳医院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol, Shenzhen Hosp,Shenzhen PKU HKUST Med Ctr, Shenzhen, Peoples R China
2.Shenzhen Univ, Urogenital Inst, Shenzhen Peoples Hosp 2, Shenzhen, Peoples R China
3.Guangzhou Panyu Cent Hosp, Guangzhou, Guangdong, Peoples R China
4.Shantou Univ Med Coll, Shantou, Peoples R China

Recommended Citation:
Yang, Ruilin,Han, Yonghua,Ye, Yiwang,et al. Chemical Synthesis of Bacteriophage G4[J]. PLOS ONE,2011,6(11).
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