IR@PKUHSC  > 基础医学院  > 免疫学系
学科主题基础医学
Rapid identification of UCA1 as a very sensitive and specific unique marker for human bladder carcinoma
Wang, Xiao-Song; Zhang, Zheng; Wang, Hong-Cheng; Cai, Jian-Liang; Xu, Qing-Wen; Li, Meng-Qiang; Chen, Yi-Cheng; Qian, Xiao-Ping; Lu, Tian-Jing; Yu, Li-Zhang; Zhang, Yu; Xin, Dian-Qi; Na, Yan-Qun; Chen, Wei-Feng
刊名CLINICAL CANCER RESEARCH
2006-08-15
DOI10.1158/1078-0432.CCR-06-0134
12期:16页:4851-4858
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]HUMAN ENDOGENOUS RETROVIRUS ; SEQUENCE-BASED AMPLIFICATION ; POLYMERASE-CHAIN-REACTION ; REVERSE-TRANSCRIPTASE ; PERIPHERAL-BLOOD ; MELANOMA-CELLS ; TUMOR-MARKER ; GENE ; EXPRESSION ; CANCER
英文摘要

Purpose: The most common genitourinary malignancy in China is bladder transitional cell carcinoma (TCC). Early diagnosis of new and recurrent bladder cancers, followed by timely treatment, will help decrease mortality. There are currently no satisfactory markers for bladder cancer available in clinics. Better diagnostic methods are highly demanded.

Experimental Design: In this research, we have used comprehensive expressed sequence tag analysis, serial analysis of gene expression, and microarray analysis and quickly discovered a candidate marker, urothelial carcinoma associated 1 (UCA1). The UCA1 gene was characterized and its performance as a urine marker was analyzed by reverse transcription-PCR with urine sediments. A total of 212 individuals were included in this study, 94 having bladder cancers, 33 ureter/pelvic cancers, and 85 normal and other urinary tract disease controls.

Results: UCA1 was identified as a novel noncoding RNA gene dramatically up-regulated in TCC and it is the most TCC-specific gene yet identified. The full-length cDNA was 1,439 bp, and sequence analysis showed that it belonged to the human endogenous retrovirus H family. Clinical tests showed that UCA1 assay was highly specific (91.8%, 78 of 85) and very sensitive (80.9%, 76 of 94) in the diagnosis of bladder cancer and was especially valuable for superficial G2-G3 patients (sensitivity 91.1%, 41 of 45). It showed excellent differential diagnostic performance in various urinary tract diseases without TCC.

Conclusions: UCA1 is a very sensitive and specific unique marker for bladder cancer. It could have important implications in postoperative noninvasive follow-up. This research also highlights a shortcut to new cancer diagnostic assays through integration of in silico isolation methods with translational clinical tests based on RNA detection protocols.

语种英语
WOS记录号WOS:000239834400010
引用统计
被引频次:172[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66772
专题基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Inst Urol, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
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GB/T 7714
Wang, Xiao-Song,Zhang, Zheng,Wang, Hong-Cheng,et al. Rapid identification of UCA1 as a very sensitive and specific unique marker for human bladder carcinoma[J]. CLINICAL CANCER RESEARCH,2006,12(16):4851-4858.
APA Wang, Xiao-Song.,Zhang, Zheng.,Wang, Hong-Cheng.,Cai, Jian-Liang.,Xu, Qing-Wen.,...&Chen, Wei-Feng.(2006).Rapid identification of UCA1 as a very sensitive and specific unique marker for human bladder carcinoma.CLINICAL CANCER RESEARCH,12(16),4851-4858.
MLA Wang, Xiao-Song,et al."Rapid identification of UCA1 as a very sensitive and specific unique marker for human bladder carcinoma".CLINICAL CANCER RESEARCH 12.16(2006):4851-4858.
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