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The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: An improved analysis using fluorescence-activated cell sorting
Zhang, Yu1,2,4; Schedle, Andreas3,4; Matejka, Michael1; Rausch-Fan, Xiaohui1; Andrukhov, Oleh1
关键词Osteoblasts Endothelial cells Co-culture Proliferation Differentiation
刊名CELLULAR & MOLECULAR BIOLOGY LETTERS
2010-12-01
DOI10.2478/s11658-010-0026-0
15期:4页:517-529
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]GROWTH-FACTOR ; HUMAN OSTEOPROGENITORS ; IN-VITRO ; EXPRESSION ; VEGF ; COMMUNICATION ; OSTEOGENESIS ; MODULATION ; PHENOTYPE ; REVEALS
英文摘要

The interaction of osteoblasts and endothelial cells plays a pivotal role in osteogenesis. This interaction has been extensively studied using their direct co-culture in vitro. However, co-culture experiments require clear discrimination between the two different cell types in the mixture, but this was rarely achieved. This study is the first to use fluorescence-activated cell sorting (FACS) for the separation and quantitative analysis of the proliferation and differentiation of MG-63 cells grown in direct co-culture with human umbilical vein endothelial cells (HUVECs). The cells of the MG-63 cell line have properties consistent with the characteristics of normal osteoblasts. We labeled HUVECs with fluorescent antibody against CD31 and used FACS to measure the proportions of each cell type and to separate them based on their different fluorescence intensities. The rate of proliferation of the MG-63 cells was estimated based on a count of the total viable cells and the proportion of MG-63 cells in the mixture. The mRNA expression levels of the osteoblast differentiation markers alkaline phosphatase (ALP), collagen type 1 (Coll-1) and osteocalcin (OC) in the MG-63 cells were measured via real-time PCR after the separation via FACS. We found that HUVECs stimulated the proliferation of the MG-63 cells after 72 h of co-culture, and inhibited it after 120 h of co-culture. The mRNA expression levels of ALP and Coll-1 significantly increased, whereas that of OC significantly decreased in MG-63 after co-culture with HUVECs. Using FACS for the quantitative analysis of the proliferation and differentiation of osteoblasts directly interacting with endothelial cells could have merit for further co-culture research.

语种英语
WOS记录号WOS:000282175000001
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66775
专题北京大学口腔医学院
作者单位1.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Hosp Stomatol, Beijing 100081, Peoples R China
3.Med Univ Vienna, Bernhard Gottlieb Univ Clin Dent, Dept Periodontol, A-1090 Vienna, Austria
4.Med Univ Vienna, Bernhard Gottlieb Univ Clin Dent, Cent Res Unit, A-1090 Vienna, Austria
推荐引用方式
GB/T 7714
Zhang, Yu,Schedle, Andreas,Matejka, Michael,et al. The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: An improved analysis using fluorescence-activated cell sorting[J]. CELLULAR & MOLECULAR BIOLOGY LETTERS,2010,15(4):517-529.
APA Zhang, Yu,Schedle, Andreas,Matejka, Michael,Rausch-Fan, Xiaohui,&Andrukhov, Oleh.(2010).The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: An improved analysis using fluorescence-activated cell sorting.CELLULAR & MOLECULAR BIOLOGY LETTERS,15(4),517-529.
MLA Zhang, Yu,et al."The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: An improved analysis using fluorescence-activated cell sorting".CELLULAR & MOLECULAR BIOLOGY LETTERS 15.4(2010):517-529.
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