|Detection of human papillomavirus in Chinese esophageal squamous cell carcinoma and its adjacent normal epithelium|
|Zhou, XB; Guo, M; Quan, LP; Zhang, W; Lu, ZM; Wang, QH; Ke, Y; Xu, NZ|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||POLYMERASE-CHAIN-REACTION ; HIGH-INCIDENCE AREA ; GENOMIC INSTABILITY ; HPV DNA ; CANCER ; INFECTION ; TYPE-16 ; ASSOCIATION ; LESIONS ; RISK|
AIM: To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal squamous cell carcinoma in China.
METHODS: Twenty-three esophageal squamous cell carcinoma samples and the distal normal epithelium from Shanxi Province, and 25 more esophageal squamous cell carcinoma samples from Anyang city, two areas with a high incidence of esophageal cancer in China, were detected for the existence of HPV-16 DNA by PCR, mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) targeting HPV-16 E6 gene.
RESULTS: There were approximately 64% (31/48) patients having HPV-16 DNA in tumor samples, among them nearly two-thirds (19/31) samples were detected with mRNA expression of HPV-16 E6. However, in the normal esophageal epithelium from cancer patients, the DNA and mRNA of HPV-16 were found with much less rate: 34.7% (8/23) and 26.1% (6/23) respectively. In addition, at protein level detected by IHC assay, 27.1% (13/48) tumor samples had virus oncoprotein E6 expression, while only one case of normal epithelium was found positive.
CONCLUSION: HPV infection, especially type 16, should be considered as a risk factor for esophageal malignancies in China.
|作者单位||1.Chinese Acad Med Sci, Canc Inst & Canc Hosp, Lab Cell & Mol Biol, Beijing 100021, Peoples R China|
2.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Genet Lab, Beijing 100034, Peoples R China
3.Third Peoples Hosp Shanxi Prov, Dept Pathol, Taiyuan 030013, Peoples R China
|Zhou, XB,Guo, M,Quan, LP,et al. Detection of human papillomavirus in Chinese esophageal squamous cell carcinoma and its adjacent normal epithelium[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(6):1170-1173.|
|APA||Zhou, XB.,Guo, M.,Quan, LP.,Zhang, W.,Lu, ZM.,...&Xu, NZ.(2003).Detection of human papillomavirus in Chinese esophageal squamous cell carcinoma and its adjacent normal epithelium.WORLD JOURNAL OF GASTROENTEROLOGY,9(6),1170-1173.|
|MLA||Zhou, XB,et al."Detection of human papillomavirus in Chinese esophageal squamous cell carcinoma and its adjacent normal epithelium".WORLD JOURNAL OF GASTROENTEROLOGY 9.6(2003):1170-1173.|