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学科主题: 临床医学
题名:
Comparison of a 3-set genotyping system with multilocus sequence typing for Streptococcus agalactiae (group B streptococcus)
作者: Sun, Y; Kong, FR; Zhao, ZT; Gilbert, GL
刊名: JOURNAL OF CLINICAL MICROBIOLOGY
发表日期: 2005-09-01
DOI: 10.1128/JCM.43.9.4704-4707.2005
卷: 43, 期:9, 页:4704-4707
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: HYLB GENE ANALYSIS ; ALPHA-C PROTEIN ; IDENTIFICATION ; COLONIZATION ; SEROTYPES ; ELEMENTS ; PCR
英文摘要:

Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Epidemiological studies of GBS infections require comprehensive typing systems that provide information about variable characteristics, such as antigenic type, virulence, or antibiotic resistance, as well as the "backbone" structure or the genetic lineage of isolates. We have previously described a 3-set genotyping system that identifies the molecular serotype (MS) or molecular serosubtype (msst), the protein gene profile, and the presence of several mobile genetic elements (F. Kong, D. Martin, G. James, and G. L. Gilbert, J. Med. Microbiol. 52:337-344, 2003). In this study, 83 clinical GBS isolates which had been previously studied by multilocus sequence typing (MLST) (N. Jones, J. F. Bohnsack, S. Takahashi, K. A. Oliver, M. S. Chan, F. Kunst, P. Glaser, C. Rusniok, D. W. Crook, R. M. Harding, N. Bisharat, and B. G. Spratt, J. Clin. Microbiol. 41:2530-2536, 2003) were examined by using the 3-set genotyping system. Genotypes were assigned to five isolates that were nontypeable by conventional serotyping. There were 27 "3-set" genotypes, 24 multilocus sequence types (STs), and 35 unique combinations (or strains), of which the 4 most common, msst III-2 (ST-17), msst III-1 (ST-19), Ia-1 (ST-23), and V-1 (ST-1), accounted for more than 60% of isolates. The 83 isolates were grouped into seven clusters, with a good correlation between the multilocus STs and the genotypes. The combination of 3-set genotyping and MLST adds discriminatory power to strain typing of GBS, which will be useful for future studies of the epidemiology and pathogenesis of GBS disease.

语种: 英语
WOS记录号: WOS:000232020400061
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66808
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Tianjin Med Univ, Coll Publ Hlth, Tianjin, Peoples R China
2.Westmead Hosp, CIDM, ICPMR, Lab Serv, Westmead, NSW 2145, Australia
3.Univ Sydney, Dept Med, Sydney, NSW 2006, Australia
4.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100871, Peoples R China

Recommended Citation:
Sun, Y,Kong, FR,Zhao, ZT,et al. Comparison of a 3-set genotyping system with multilocus sequence typing for Streptococcus agalactiae (group B streptococcus)[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2005,43(9):4704-4707.
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