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Comparison of a 3-set genotyping system with multilocus sequence typing for Streptococcus agalactiae (group B streptococcus)
Sun, Y; Kong, FR; Zhao, ZT; Gilbert, GL
刊名JOURNAL OF CLINICAL MICROBIOLOGY
2005-09-01
DOI10.1128/JCM.43.9.4704-4707.2005
43期:9页:4704-4707
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]HYLB GENE ANALYSIS ; ALPHA-C PROTEIN ; IDENTIFICATION ; COLONIZATION ; SEROTYPES ; ELEMENTS ; PCR
英文摘要

Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Epidemiological studies of GBS infections require comprehensive typing systems that provide information about variable characteristics, such as antigenic type, virulence, or antibiotic resistance, as well as the "backbone" structure or the genetic lineage of isolates. We have previously described a 3-set genotyping system that identifies the molecular serotype (MS) or molecular serosubtype (msst), the protein gene profile, and the presence of several mobile genetic elements (F. Kong, D. Martin, G. James, and G. L. Gilbert, J. Med. Microbiol. 52:337-344, 2003). In this study, 83 clinical GBS isolates which had been previously studied by multilocus sequence typing (MLST) (N. Jones, J. F. Bohnsack, S. Takahashi, K. A. Oliver, M. S. Chan, F. Kunst, P. Glaser, C. Rusniok, D. W. Crook, R. M. Harding, N. Bisharat, and B. G. Spratt, J. Clin. Microbiol. 41:2530-2536, 2003) were examined by using the 3-set genotyping system. Genotypes were assigned to five isolates that were nontypeable by conventional serotyping. There were 27 "3-set" genotypes, 24 multilocus sequence types (STs), and 35 unique combinations (or strains), of which the 4 most common, msst III-2 (ST-17), msst III-1 (ST-19), Ia-1 (ST-23), and V-1 (ST-1), accounted for more than 60% of isolates. The 83 isolates were grouped into seven clusters, with a good correlation between the multilocus STs and the genotypes. The combination of 3-set genotyping and MLST adds discriminatory power to strain typing of GBS, which will be useful for future studies of the epidemiology and pathogenesis of GBS disease.

语种英语
WOS记录号WOS:000232020400061
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66808
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Tianjin Med Univ, Coll Publ Hlth, Tianjin, Peoples R China
2.Westmead Hosp, CIDM, ICPMR, Lab Serv, Westmead, NSW 2145, Australia
3.Univ Sydney, Dept Med, Sydney, NSW 2006, Australia
4.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100871, Peoples R China
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GB/T 7714
Sun, Y,Kong, FR,Zhao, ZT,et al. Comparison of a 3-set genotyping system with multilocus sequence typing for Streptococcus agalactiae (group B streptococcus)[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2005,43(9):4704-4707.
APA Sun, Y,Kong, FR,Zhao, ZT,&Gilbert, GL.(2005).Comparison of a 3-set genotyping system with multilocus sequence typing for Streptococcus agalactiae (group B streptococcus).JOURNAL OF CLINICAL MICROBIOLOGY,43(9),4704-4707.
MLA Sun, Y,et al."Comparison of a 3-set genotyping system with multilocus sequence typing for Streptococcus agalactiae (group B streptococcus)".JOURNAL OF CLINICAL MICROBIOLOGY 43.9(2005):4704-4707.
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