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学科主题: 临床医学
题名:
Subcellullar localization of tumor-associated antigen 3H11Ag
作者: Guo, JH; Jin, GL; Meng, L; Ma, H; Nie, DZ; Wu, J; Yuan, L; Shou, CC
关键词: subcellular localization ; 3H11Ag ; BLAST ; PSORT II ; coiled coil ; cell fractionation
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2004-11-12
DOI: 10.1016/j.bbrc.2004.09.133
卷: 324, 期:2, 页:922-930
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: PORE-TARGETING COMPLEX ; MONOCLONAL-ANTIBODY 3H11 ; NUCLEAR-PROTEIN IMPORT ; 3 DISTINCT CLASSES ; SACCHAROMYCES-CEREVISIAE ; COILED COILS ; BINDING ; SIGNAL ; IDENTIFICATION ; MATRIX
英文摘要:

3H11Ag, a tumor-associated antigen defined by the monoclonal antibody 3H11 that specifically recognizes cancer cells in various tumor tissues, was successfully cloned recently, but its function is unknown. To explore the potential roles it plays in tumors, we analyzed its subcellular localization in the present study. By expressing 3H11Ag fused with fluorescent protein in COS-7 cells, we found that 3H11Ag localizes to both cytoplasm and nucleus, which was confirmed by subcellular fractionation. And sequentially extracting the nuclei of COS-7 cells transfected with 3H11Ag showed that it is a DNA- and nuclear matrix-associated protein. Moreover, by expressing a series of red fluorescent protein-tagged truncated forms of 3H11Ag, it was demonstrated that the 150 amino acid residues at its C-terminal are fully responsible for the subcellular localization. In addition, the results of the computational analysis of 3H11Ag were in accordance with those of the experimental analysis. All these data would be helpful to elucidate the functions of 3H11Ag. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000224794000066
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66829
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
2.Peking Univ, Med Anal Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Guo, JH,Jin, GL,Meng, L,et al. Subcellullar localization of tumor-associated antigen 3H11Ag[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,324(2):922-930.
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