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Subcellullar localization of tumor-associated antigen 3H11Ag
Guo, JH; Jin, GL; Meng, L; Ma, H; Nie, DZ; Wu, J; Yuan, L; Shou, CC
关键词subcellular localization 3H11Ag BLAST PSORT II coiled coil cell fractionation
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2004-11-12
DOI10.1016/j.bbrc.2004.09.133
324期:2页:922-930
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]PORE-TARGETING COMPLEX ; MONOCLONAL-ANTIBODY 3H11 ; NUCLEAR-PROTEIN IMPORT ; 3 DISTINCT CLASSES ; SACCHAROMYCES-CEREVISIAE ; COILED COILS ; BINDING ; SIGNAL ; IDENTIFICATION ; MATRIX
英文摘要

3H11Ag, a tumor-associated antigen defined by the monoclonal antibody 3H11 that specifically recognizes cancer cells in various tumor tissues, was successfully cloned recently, but its function is unknown. To explore the potential roles it plays in tumors, we analyzed its subcellular localization in the present study. By expressing 3H11Ag fused with fluorescent protein in COS-7 cells, we found that 3H11Ag localizes to both cytoplasm and nucleus, which was confirmed by subcellular fractionation. And sequentially extracting the nuclei of COS-7 cells transfected with 3H11Ag showed that it is a DNA- and nuclear matrix-associated protein. Moreover, by expressing a series of red fluorescent protein-tagged truncated forms of 3H11Ag, it was demonstrated that the 150 amino acid residues at its C-terminal are fully responsible for the subcellular localization. In addition, the results of the computational analysis of 3H11Ag were in accordance with those of the experimental analysis. All these data would be helpful to elucidate the functions of 3H11Ag. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000224794000066
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66829
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
2.Peking Univ, Med Anal Ctr, Beijing 100871, Peoples R China
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Guo, JH,Jin, GL,Meng, L,et al. Subcellullar localization of tumor-associated antigen 3H11Ag[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,324(2):922-930.
APA Guo, JH.,Jin, GL.,Meng, L.,Ma, H.,Nie, DZ.,...&Shou, CC.(2004).Subcellullar localization of tumor-associated antigen 3H11Ag.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,324(2),922-930.
MLA Guo, JH,et al."Subcellullar localization of tumor-associated antigen 3H11Ag".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 324.2(2004):922-930.
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