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学科主题: 基础医学
题名:
Low expression of miR-150 in pediatric intestinal Burkitt lymphoma
作者: Wang, Miao1; Yang, Wenping2; Li, Min3; Li, Yong1
关键词: Burkitt lymphoma ; Reactive lymphoid hyperplasia ; miR-150 ; qRT-PCR ; ISH
刊名: EXPERIMENTAL AND MOLECULAR PATHOLOGY
发表日期: 2014-04-01
DOI: 10.1016/j.yexmp.2014.02.015
卷: 96, 期:2, 页:261-266
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pathology
研究领域[WOS]: Pathology
关键词[WOS]: C-MYB ; CELL LYMPHOMA ; MIRNA PROFILE ; HUMAN CANCERS ; DIFFERENTIATION ; MICRORNA-150 ; CHEMOTHERAPY ; CONTRIBUTES ; PROGNOSIS
英文摘要:

Background: Burkitt lymphoma (BL) is a highly aggressive B-cell lymphoma with rapid proliferation. It has become evident that miRNAs are involved in hematopoietic malignancies. This study was undertaken to investigate the miRNA expression patterns of pediatric intestinal BL tissues.

Methods: We collected 28 BL and 8 reactive lymphoid hyperplasia (RLH) samples. miRNA expression profiling was performed in BL and RLH tissues to identify BL-related miRNAs, which were further analyzed by qRT-PCR and miRNA-ISH. In addition, immunohistochemistry (IHC) and westem blot were used to define the protein targets of the BL-related miRNAs. Furthermore, we evaluated cell growth status by using methylthiazolyldiphenyltetrazolium bromide (MU) assay in Raji cell line, which was transected with the BL-related miRNA mimics or inhibitors.

Results: miRNA expression profiling showed that miR-150 had extremely decreased expression levels in BL patients. In both ISH and qRT-PCR analyses, BL had reduced levels of miR-150 expression compared with RLH. However, there is no significant correlation of miR-150 expression and EBV status in BL Moreover, IHC and western blotting defined that c-Myb and Survivin are the protein targets of miR-150. Re-expression of miR-150 reduced the proliferation of Raji cells.

Conclusions: Deregulation of miR-150 may be useful as a diagnostic tool in BL, based on miRNA profile screening, qRT-PCR and miRNA-ISH. miR-150 plays an important role in BL by targeting c-Myb and Survivin. Re-expression of miR-150 reduced the proliferation of Raji cells, which suggests it to be a promising novel candidate for tumor treatment. (C) 2014 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 81001054
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000334655100021
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66841
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Jiangxi Childrens Hosp, Dept Pathol, Jiangxi, Peoples R China
2.Capital Med Univ, Basic Med Coll, Dept Pathol, Beijing 100069, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Basic Med Coll, Dept Pathol, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Miao,Yang, Wenping,Li, Min,et al. Low expression of miR-150 in pediatric intestinal Burkitt lymphoma[J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY,2014,96(2):261-266.
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