|Low expression of miR-150 in pediatric intestinal Burkitt lymphoma|
|Wang, Miao1; Yang, Wenping2; Li, Min3; Li, Yong1|
|关键词||Burkitt lymphoma Reactive lymphoid hyperplasia miR-150 qRT-PCR ISH|
|刊名||EXPERIMENTAL AND MOLECULAR PATHOLOGY|
|WOS标题词||Science & Technology|
|关键词[WOS]||C-MYB ; CELL LYMPHOMA ; MIRNA PROFILE ; HUMAN CANCERS ; DIFFERENTIATION ; MICRORNA-150 ; CHEMOTHERAPY ; CONTRIBUTES ; PROGNOSIS|
Background: Burkitt lymphoma (BL) is a highly aggressive B-cell lymphoma with rapid proliferation. It has become evident that miRNAs are involved in hematopoietic malignancies. This study was undertaken to investigate the miRNA expression patterns of pediatric intestinal BL tissues.
Methods: We collected 28 BL and 8 reactive lymphoid hyperplasia (RLH) samples. miRNA expression profiling was performed in BL and RLH tissues to identify BL-related miRNAs, which were further analyzed by qRT-PCR and miRNA-ISH. In addition, immunohistochemistry (IHC) and westem blot were used to define the protein targets of the BL-related miRNAs. Furthermore, we evaluated cell growth status by using methylthiazolyldiphenyltetrazolium bromide (MU) assay in Raji cell line, which was transected with the BL-related miRNA mimics or inhibitors.
Results: miRNA expression profiling showed that miR-150 had extremely decreased expression levels in BL patients. In both ISH and qRT-PCR analyses, BL had reduced levels of miR-150 expression compared with RLH. However, there is no significant correlation of miR-150 expression and EBV status in BL Moreover, IHC and western blotting defined that c-Myb and Survivin are the protein targets of miR-150. Re-expression of miR-150 reduced the proliferation of Raji cells.
Conclusions: Deregulation of miR-150 may be useful as a diagnostic tool in BL, based on miRNA profile screening, qRT-PCR and miRNA-ISH. miR-150 plays an important role in BL by targeting c-Myb and Survivin. Re-expression of miR-150 reduced the proliferation of Raji cells, which suggests it to be a promising novel candidate for tumor treatment. (C) 2014 Elsevier Inc. All rights reserved.
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Jiangxi Childrens Hosp, Dept Pathol, Jiangxi, Peoples R China|
2.Capital Med Univ, Basic Med Coll, Dept Pathol, Beijing 100069, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Basic Med Coll, Dept Pathol, Beijing 100871, Peoples R China
|Wang, Miao,Yang, Wenping,Li, Min,et al. Low expression of miR-150 in pediatric intestinal Burkitt lymphoma[J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY,2014,96(2):261-266.|
|APA||Wang, Miao,Yang, Wenping,Li, Min,&Li, Yong.(2014).Low expression of miR-150 in pediatric intestinal Burkitt lymphoma.EXPERIMENTAL AND MOLECULAR PATHOLOGY,96(2),261-266.|
|MLA||Wang, Miao,et al."Low expression of miR-150 in pediatric intestinal Burkitt lymphoma".EXPERIMENTAL AND MOLECULAR PATHOLOGY 96.2(2014):261-266.|
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