IR@PKUHSC  > 北京大学第一临床医学院  > 心血管内科
学科主题临床医学
Cortistatin attenuates vascular calcification in rats
Liu, Yue1; Zhou, Ye Bo2; Zhang, Gai Gai1; Cai, Yan2; Duan, Xiao Hui2; Teng, Xu2; Song, Jun Qiu2; Shi, Yi2; Tang, Chao Shu2,3,4; Yin, Xin Hua1; Qi, Yong Fen2,3,4
关键词Cortistatin Vascular calcification Receptor Rat
刊名REGULATORY PEPTIDES
2010-01-08
DOI10.1016/j.regpep.2009.09.005
159期:1-3页:35-43
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Physiology
资助者Major Basic Research Program of China ; National Natural Science Foundation of China ; Major Basic Research Program of China ; National Natural Science Foundation of China
研究领域[WOS]Endocrinology & Metabolism ; Physiology
关键词[WOS]SMOOTH-MUSCLE-CELLS ; SOMATOSTATIN RECEPTORS ; IN-VITRO ; DISEASE ; NEUROPEPTIDE ; MECHANISMS ; EXPRESSION ; RESPONSES ; NICOTINE ; GHRELIN
英文摘要

Cortistatin (CST) is a newly discovered polypeptide with multiple biological activities that plays a regulatory role in the nervous, endocrine and immune systems. However, the role of CST in the pathogenesis of cardiovascular diseases remains unclear. In this study, we investigated in rats whether CST inhibits vascular calcification induced by vitamin D3 and nicotine treatment in vivo and calcification of cultured rat vascular smooth muscular cells (VSMCs) induced by beta-glycerophosphate in vitro and the underlying mechanism. We measured rat hemodynamic variables, alkaline phosphatase (ALP) activity, calcium deposition and pathological changes in aortic tissues and cultured VSMCs. CST treatment significantly improved hemodynamic values and arterial compliance in rats with vascular calcification by decreasing systolic, blood pressure, pulse pressure, left ventricular end-systolic pressure and left ventricular end-diastolic pressure. CST also significantly decreased ALP activity and calcium deposition, alleviated pathological injury and down-regulated the mRNA expression of type III sodium-dependent phosphate co-transporter-1 (Pit-1) in aortic tissues. It dose-independently inhibited the calcification of VSMCs by decreasing ALP activity and calcium deposition, alleviating pathologic injury and down-regulating Pit-1 mRNA expression. As with CST treatment, ALP activation and calcium deposition were decreased significantly on treatment with ghrelin, the endogenous agonist of growth hormone secretagogue receptor 1a (CHSR1a), but not significantly with somatostatin-14 or proadrenomedullin N-terminal 20 peptide in VSMCs. Further, growth hormone-releasing peptide-6[D-lys], the endogenous antagonist of GHSR1a, markedly reversed the increased ALP activity and calcium deposition in VSMCs. CST could be a new target molecule for the prevention and therapy of vascular calcification, whose effects are mediated by GHSR1a rather than SSTRs or Mrg X2. (C) 2009 Elsevier B.V. All rights reserved.

语种英语
所属项目编号2006CB503807 ; 30670847 ; 30871013 ; 30470693
资助者Major Basic Research Program of China ; National Natural Science Foundation of China ; Major Basic Research Program of China ; National Natural Science Foundation of China
WOS记录号WOS:000273920500007
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66853
Collection北京大学第一临床医学院_心血管内科
作者单位1.Harbin Med Univ, Affiliated Hosp 1, Dept Cardiol, Harbin 150086, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
3.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
4.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yue,Zhou, Ye Bo,Zhang, Gai Gai,et al. Cortistatin attenuates vascular calcification in rats[J]. REGULATORY PEPTIDES,2010,159(1-3):35-43.
APA Liu, Yue.,Zhou, Ye Bo.,Zhang, Gai Gai.,Cai, Yan.,Duan, Xiao Hui.,...&Qi, Yong Fen.(2010).Cortistatin attenuates vascular calcification in rats.REGULATORY PEPTIDES,159(1-3),35-43.
MLA Liu, Yue,et al."Cortistatin attenuates vascular calcification in rats".REGULATORY PEPTIDES 159.1-3(2010):35-43.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Liu, Yue]'s Articles
[Zhou, Ye Bo]'s Articles
[Zhang, Gai Gai]'s Articles
百度学术
百度学术Similar articles in
[Liu, Yue]'s Articles
[Zhou, Ye Bo]'s Articles
[Zhang, Gai Gai]'s Articles
必应学术
必应学术Similar articles in
[Liu, Yue]'s Articles
[Zhou, Ye Bo]'s Articles
[Zhang, Gai Gai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.