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学科主题: 口腔医学
题名:
Zinc Enhances Bone Metabolism in Ovariectomized Rats and Exerts Anabolic Osteoblastic/Adipocytic Marrow Effects Ex Vivo
作者: Li, Binbin1; Liu, Hao2; Jia, Shengnan1
关键词: Zn ; Bone ; Ovariectomy ; Osteoblastogenesis ; Marrow adiposity
刊名: BIOLOGICAL TRACE ELEMENT RESEARCH
发表日期: 2015-02-01
DOI: 10.1007/s12011-014-0185-3
卷: 163, 期:1-2, 页:202-207
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism
研究领域[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism
关键词[WOS]: MINERAL DENSITY ; CULTURE ; TISSUE ; CELLS ; EXPRESSION ; OSTEOCLASTOGENESIS ; OSTEOPOROSIS ; DEFICIENCY ; MC3T3-E1 ; OBESITY
英文摘要:

Investigations of bone mass and marrow adiposity are critical for defining the role of zinc (Zn) in bone metabolism. Rats used for study were grouped as follows: control (sham), ovariectomy (OVX), ovariectomy + estradiol (OVX-E), ovariectomy + Zn treatment (OVX-Zn). Bone mineral density (BMD) was quantified (microCT); serum osteocalcin, adiponectin, RANKL, and TRAP levels were assayed (ELISA); and biochemical determinations of serum alkaline phosphatase (ALP), calcium (Ca), and phosphorus (P) were done. Cells derived from bone mesenchymal stem cell (BMSC) isolates of respective test groups were compared, identifying primary osteoblasts by MTT assay and adipocytes by Oil Red O stain. Osteocalcin and adiponectin levels in culture supernatants were determined by ELISA. Zn supplementation resulted in a modest increase in BMD, but serum osteocalcin and ALP activity increased significantly (P < 0.01, both). Serum levels of RANKL and TRAP were lower in OVX-Zn (vs OVX) rats (P < 0.01), whereas serum concentrations of adiponectin, Ca, and P did not differ by group. Osteocalcin level was significantly upregulated ex vivo (P < 0.01) in the supernatant of cultured OVX-Zn (vs OVX) cells, accompanied by a slight upturn in osteoblastic differentiation. However, Oil Red O uptake and adiponectin level in supernatant were sharply diminished in cultured OVX-Zn (vs OVX) cells (P < 0.01). Overall, we concluded that Zn contributes to bone mass by marginally stimulating differentiation and proliferation of osteoblasts and by effectively inhibiting osteoclastic and adipocytic differentiation of BMSCs.

语种: 英语
所属项目编号: 30901671
项目资助者: National Natural Science Foundations of China, Beijing, China
WOS记录号: WOS:000348109500025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66873
Appears in Collections:北京大学口腔医学院_口腔病理科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Li, Binbin,Liu, Hao,Jia, Shengnan. Zinc Enhances Bone Metabolism in Ovariectomized Rats and Exerts Anabolic Osteoblastic/Adipocytic Marrow Effects Ex Vivo[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2015,163(1-2):202-207.
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