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学科主题口腔医学
Zinc Enhances Bone Metabolism in Ovariectomized Rats and Exerts Anabolic Osteoblastic/Adipocytic Marrow Effects Ex Vivo
Li, Binbin1; Liu, Hao2; Jia, Shengnan1
关键词Zn Bone Ovariectomy Osteoblastogenesis Marrow adiposity
刊名BIOLOGICAL TRACE ELEMENT RESEARCH
2015-02-01
DOI10.1007/s12011-014-0185-3
163期:1-2页:202-207
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Endocrinology & Metabolism
资助者National Natural Science Foundations of China, Beijing, China ; National Natural Science Foundations of China, Beijing, China
研究领域[WOS]Biochemistry & Molecular Biology ; Endocrinology & Metabolism
关键词[WOS]MINERAL DENSITY ; CULTURE ; TISSUE ; CELLS ; EXPRESSION ; OSTEOCLASTOGENESIS ; OSTEOPOROSIS ; DEFICIENCY ; MC3T3-E1 ; OBESITY
英文摘要

Investigations of bone mass and marrow adiposity are critical for defining the role of zinc (Zn) in bone metabolism. Rats used for study were grouped as follows: control (sham), ovariectomy (OVX), ovariectomy + estradiol (OVX-E), ovariectomy + Zn treatment (OVX-Zn). Bone mineral density (BMD) was quantified (microCT); serum osteocalcin, adiponectin, RANKL, and TRAP levels were assayed (ELISA); and biochemical determinations of serum alkaline phosphatase (ALP), calcium (Ca), and phosphorus (P) were done. Cells derived from bone mesenchymal stem cell (BMSC) isolates of respective test groups were compared, identifying primary osteoblasts by MTT assay and adipocytes by Oil Red O stain. Osteocalcin and adiponectin levels in culture supernatants were determined by ELISA. Zn supplementation resulted in a modest increase in BMD, but serum osteocalcin and ALP activity increased significantly (P < 0.01, both). Serum levels of RANKL and TRAP were lower in OVX-Zn (vs OVX) rats (P < 0.01), whereas serum concentrations of adiponectin, Ca, and P did not differ by group. Osteocalcin level was significantly upregulated ex vivo (P < 0.01) in the supernatant of cultured OVX-Zn (vs OVX) cells, accompanied by a slight upturn in osteoblastic differentiation. However, Oil Red O uptake and adiponectin level in supernatant were sharply diminished in cultured OVX-Zn (vs OVX) cells (P < 0.01). Overall, we concluded that Zn contributes to bone mass by marginally stimulating differentiation and proliferation of osteoblasts and by effectively inhibiting osteoclastic and adipocytic differentiation of BMSCs.

语种英语
所属项目编号30901671
资助者National Natural Science Foundations of China, Beijing, China ; National Natural Science Foundations of China, Beijing, China
WOS记录号WOS:000348109500025
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66873
专题北京大学口腔医学院_口腔病理科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Li, Binbin,Liu, Hao,Jia, Shengnan. Zinc Enhances Bone Metabolism in Ovariectomized Rats and Exerts Anabolic Osteoblastic/Adipocytic Marrow Effects Ex Vivo[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2015,163(1-2):202-207.
APA Li, Binbin,Liu, Hao,&Jia, Shengnan.(2015).Zinc Enhances Bone Metabolism in Ovariectomized Rats and Exerts Anabolic Osteoblastic/Adipocytic Marrow Effects Ex Vivo.BIOLOGICAL TRACE ELEMENT RESEARCH,163(1-2),202-207.
MLA Li, Binbin,et al."Zinc Enhances Bone Metabolism in Ovariectomized Rats and Exerts Anabolic Osteoblastic/Adipocytic Marrow Effects Ex Vivo".BIOLOGICAL TRACE ELEMENT RESEARCH 163.1-2(2015):202-207.
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