北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 生物物理学系  > 期刊论文
学科主题: 基础医学
题名:
beta-turn formation by a six-residue linear peptide in solution
作者: Gao, F; Wang, Y; Qiu, Y; Li, Y; Sha, Y; Lai, L; Wu, H
关键词: beta-turn ; CD ; FT-IR ; NMR
刊名: JOURNAL OF PEPTIDE RESEARCH
发表日期: 2002-08-01
卷: 60, 期:2, 页:75-80
收录类别: SCI ; IC
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: PROTEIN SECONDARY STRUCTURE ; INFRARED-SPECTROSCOPY ; HAIRPIN ; PROGRAM ; MACROMOLECULES ; INHIBITOR ; STABILITY ; UBIQUITIN ; SYSTEM ; ACIDS
英文摘要:

A model peptide AAGDYY-NH2 (beta1), which is found to adopt a beta-turn conformation in the TEM-1 beta-lactamase inhibitor protein (BLIP) in the TEM-1/BLIP co-crystal, was synthesized to elucidate the mechanism of its beta-turn formation and stability. Its structural preferences in solution were comprehensively characterized using CD, FT-IR and H-1 NMR spectroscopy, respectively. The set of observed diagnostic NOEs, the restrained molecular dynamics simulation, CD and FT-IR spectroscopy confirmed the formation of a beta-turn in solution by the model peptide. The dihedral angles [(phi(3), phi(3)) (phi(4), phi(4))] of [(-52degrees, -32degrees) (-38degrees, -44degrees)] of Gly-Asp fragment in the model peptide are consistent with those of a type III beta-turn. in a conclusion, the conformational preference of the linear hexapeptide beta1 in solution was determined, and it would provide a simple template to study the mechanism of beta-turn formation and stability.

语种: 英语
WOS记录号: WOS:000177120000001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66882
Appears in Collections:基础医学院_生物物理学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Inst Phys Chem, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biophys, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioogan & Nat Prod Chem, Shanghai, Peoples R China

Recommended Citation:
Gao, F,Wang, Y,Qiu, Y,et al. beta-turn formation by a six-residue linear peptide in solution[J]. JOURNAL OF PEPTIDE RESEARCH,2002,60(2):75-80.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, F]'s Articles
[Wang, Y]'s Articles
[Qiu, Y]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, F]‘s Articles
[Wang, Y]‘s Articles
[Qiu, Y]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace