IR@PKUHSC  > 北京大学基础医学院  > 生物物理学系
学科主题基础医学
beta-turn formation by a six-residue linear peptide in solution
Gao, F; Wang, Y; Qiu, Y; Li, Y; Sha, Y; Lai, L; Wu, H
关键词beta-turn CD FT-IR NMR
刊名JOURNAL OF PEPTIDE RESEARCH
2002-08-01
60期:2页:75-80
收录类别SCI ; IC
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]PROTEIN SECONDARY STRUCTURE ; INFRARED-SPECTROSCOPY ; HAIRPIN ; PROGRAM ; MACROMOLECULES ; INHIBITOR ; STABILITY ; UBIQUITIN ; SYSTEM ; ACIDS
英文摘要

A model peptide AAGDYY-NH2 (beta1), which is found to adopt a beta-turn conformation in the TEM-1 beta-lactamase inhibitor protein (BLIP) in the TEM-1/BLIP co-crystal, was synthesized to elucidate the mechanism of its beta-turn formation and stability. Its structural preferences in solution were comprehensively characterized using CD, FT-IR and H-1 NMR spectroscopy, respectively. The set of observed diagnostic NOEs, the restrained molecular dynamics simulation, CD and FT-IR spectroscopy confirmed the formation of a beta-turn in solution by the model peptide. The dihedral angles [(phi(3), phi(3)) (phi(4), phi(4))] of [(-52degrees, -32degrees) (-38degrees, -44degrees)] of Gly-Asp fragment in the model peptide are consistent with those of a type III beta-turn. in a conclusion, the conformational preference of the linear hexapeptide beta1 in solution was determined, and it would provide a simple template to study the mechanism of beta-turn formation and stability.

语种英语
WOS记录号WOS:000177120000001
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66882
专题北京大学基础医学院_生物物理学系
作者单位1.Peking Univ, Inst Phys Chem, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biophys, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioogan & Nat Prod Chem, Shanghai, Peoples R China
推荐引用方式
GB/T 7714
Gao, F,Wang, Y,Qiu, Y,et al. beta-turn formation by a six-residue linear peptide in solution[J]. JOURNAL OF PEPTIDE RESEARCH,2002,60(2):75-80.
APA Gao, F.,Wang, Y.,Qiu, Y.,Li, Y.,Sha, Y.,...&Wu, H.(2002).beta-turn formation by a six-residue linear peptide in solution.JOURNAL OF PEPTIDE RESEARCH,60(2),75-80.
MLA Gao, F,et al."beta-turn formation by a six-residue linear peptide in solution".JOURNAL OF PEPTIDE RESEARCH 60.2(2002):75-80.
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