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学科主题: 基础医学
题名:
beta-turn formation by a six-residue linear peptide in solution
作者: Gao, F; Wang, Y; Qiu, Y; Li, Y; Sha, Y; Lai, L; Wu, H
关键词: beta-turn ; CD ; FT-IR ; NMR
刊名: JOURNAL OF PEPTIDE RESEARCH
发表日期: 2002-08-01
卷: 60, 期:2, 页:75-80
收录类别: SCI ; IC
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: PROTEIN SECONDARY STRUCTURE ; INFRARED-SPECTROSCOPY ; HAIRPIN ; PROGRAM ; MACROMOLECULES ; INHIBITOR ; STABILITY ; UBIQUITIN ; SYSTEM ; ACIDS
英文摘要:

A model peptide AAGDYY-NH2 (beta1), which is found to adopt a beta-turn conformation in the TEM-1 beta-lactamase inhibitor protein (BLIP) in the TEM-1/BLIP co-crystal, was synthesized to elucidate the mechanism of its beta-turn formation and stability. Its structural preferences in solution were comprehensively characterized using CD, FT-IR and H-1 NMR spectroscopy, respectively. The set of observed diagnostic NOEs, the restrained molecular dynamics simulation, CD and FT-IR spectroscopy confirmed the formation of a beta-turn in solution by the model peptide. The dihedral angles [(phi(3), phi(3)) (phi(4), phi(4))] of [(-52degrees, -32degrees) (-38degrees, -44degrees)] of Gly-Asp fragment in the model peptide are consistent with those of a type III beta-turn. in a conclusion, the conformational preference of the linear hexapeptide beta1 in solution was determined, and it would provide a simple template to study the mechanism of beta-turn formation and stability.

语种: 英语
WOS记录号: WOS:000177120000001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66882
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Inst Phys Chem, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biophys, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioogan & Nat Prod Chem, Shanghai, Peoples R China

Recommended Citation:
Gao, F,Wang, Y,Qiu, Y,et al. beta-turn formation by a six-residue linear peptide in solution[J]. JOURNAL OF PEPTIDE RESEARCH,2002,60(2):75-80.
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