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Overexpression of parathyroid pituitary-specific transcription factor (Pit)-1 in hyperphosphatemia-induced hyperparathyroidism of chronic renal failure rats
Jiang Ying; Wang Mei
关键词pituitary-specific transcription factor-1 (Pit-1) hyperphosphatemia secondary hyperparathyroidism chronic renal failure
刊名CHINESE MEDICAL JOURNAL
2010-06-20
DOI10.3760/cma.j.issn.0366-6999.2010.12.016
123期:12页:1566-1570
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]OPOSSUM KIDNEY-CELLS ; SECONDARY HYPERPARATHYROIDISM ; PHOSPHATE COTRANSPORTERS ; IN-VITRO ; PIT-1 ; TRANSPORT ; SECRETION
英文摘要

Background Hyperphosphatemia in renal failure has been identified as a major role in the pathogenesis of hyperparathyroidism that is independent of changes in serum calcium and 1,25(OH)(2)D(3). The aim of this study was to evaluate the expression of parathyroid Pit-1 in hyperphosphatemia-induced secondary hyperparathyroidism (SHPT) of chronic renal failure (CRF) rats.

Methods Wistar rats with CRF induced by 5/6 nephrectomy were ramdomly fed with diet containing 1.2% inorganic phosphate (Pi, high phosphate (HP) group, n=9) or 0.2% Pi (low phosphate (LP) group, n=9) for 10 weeks starting from the fourth week after the surgery. Another 7 nephrectomy rats with HP diet were intraperitoneally injected with phosphonoformic acid (PFA, the specific inhibitor of Pit-1, HP+PFA group) 0.15 g/kg every other day for 10 weeks starting from HP diet. Another 6 HP rats injected with the same amount of normal saline as the control of the HP+PFA group (HP+saline group). At the same time, 9 rats with sham surgery received HP diet as the controls. At the 4th week and 14th week, blood was taken for measurement of serum creatinine (SCr), serum calcium (SCa), serum phosphorus (SPi), 1,25(OH)(2)D(3) and intact parathyroid hormone (iPTH). At the 14th week, two parathroid glands (PTGs) of each rat were removed by microsurgery, one gland for immunohistochemistry analysis of proliferating cell nuclear antigen (PCNA), the other one for detection of Pit-1 by Western blotting, and for the measurement of Pit-1 mRNA and PTH mRNA by real-time quantitative polymerase chain reaction.

Results In nephrectomy rats, high dierary phosphate induced a marked increase in serum phosphate, iPTH, PTH mRNA and PCNA parathyroid cells, accompanying Pit-1 and its mRNA in parathyroid gland increased significantly. However, serum Ca and 1,25(OH)(2)D(3) remained unchanged. PEA decreased Pit-1 and its mRNA levels to reduce intact PTH, PTH mRNA and PCNA-positive parathyroid cells.

Conclusions Expression of parathyroid Pit-1 in hyperphosphatemia-induced SHPT of CRF rats was upregulated. Pit-1 may mediate the stimulation to parathyroid gland by hyperphosphatemia. Chin Med J 2010;123(12):1566-1570

语种英语
WOS记录号WOS:000279754100016
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66887
专题北京大学第二临床医学院_肾内科
北京大学口腔医学院_外科
作者单位Peking Univ, Peoples Hosp, Dept Nephrol, Beijing 100044, Peoples R China
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Jiang Ying,Wang Mei. Overexpression of parathyroid pituitary-specific transcription factor (Pit)-1 in hyperphosphatemia-induced hyperparathyroidism of chronic renal failure rats[J]. CHINESE MEDICAL JOURNAL,2010,123(12):1566-1570.
APA Jiang Ying,&Wang Mei.(2010).Overexpression of parathyroid pituitary-specific transcription factor (Pit)-1 in hyperphosphatemia-induced hyperparathyroidism of chronic renal failure rats.CHINESE MEDICAL JOURNAL,123(12),1566-1570.
MLA Jiang Ying,et al."Overexpression of parathyroid pituitary-specific transcription factor (Pit)-1 in hyperphosphatemia-induced hyperparathyroidism of chronic renal failure rats".CHINESE MEDICAL JOURNAL 123.12(2010):1566-1570.
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