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Effects of black cohosh and estrogen on the hypothalamic nuclei of ovariectomized rats at different temperatures
Zhang Hui2; Ma Xiaoyan2; Yang Mukun2; Wang Ke1; Yang Liyuan1; Zhu Sainan3; Jia Jing; Qin Lihua1; Bai Wenpei2
关键词Black cohosh Estrogen Temperature stimulation c-Fos protein Ovariectomized rats Immunohistochemistry
刊名JOURNAL OF ETHNOPHARMACOLOGY
2012-08-01
DOI10.1016/j.jep.2012.05.059
142期:3页:769-775
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]CLIMACTERIC COMPLAINTS ; CIMICIFUGA-RACEMOSA ; EXTRACT BNO-1055 ; GENE-EXPRESSION ; ADIPOSE-TISSUE ; COLD-EXPOSURE ; DOUBLE-BLIND ; HOT FLUSHES ; EFFICACY ; THERMOREGULATION
英文摘要

Ethnopharmacological relevance: Cimicifuga racemosa (L) Nutt. (CR), known as black cohosh, has been used in Europe as a medicinal plant for more than a century and its roots have been widely used for the treatment of menopausal symptoms. Remifemin, the main ingredient in liquid or tablet medications prepared from isopropyl alcohol extracts of black cohosh rhizome, has also been evaluated in clinical studies.

Objectives: To observe changes in the expression of the c-Fos protein in the hypothalamic nuclei of four groups of rats sham-operated group (SHAM), ovariectomized (OVX) group, ovariectomized group treated with estrogen(OVX+E), and ovariectomized group treated with the isopropanol extract of Cimicifuga racemosa (OVX+ICR)-and to investigate the mechanisms of black cohosh and estrogen that take place in the hypothalamic nuclei of ovariectomized rats.

Methods: Fifty rats were assigned to each of the four groups and placed in incubators at 4 degrees C, 10 degrees C, 25 degrees C, 33 degrees C, or 38 degrees C for 2 h. They were then anesthetized, and their brains were removed after heart perfusion. c-Fos expression in the hypothalamic nuclei was evaluated using immunohistochemical methods.

Results: In the median preoptic nucleus (MnPO), ventromedial preoptic nucleus (VMPO), and suprachiasmatic nucleus (SCh) of the SHAM group, in the anterior hypothalamic area (AH) and supraoptic nucleus (SO) of all four groups, and in the paraventricular nucleus (PVN) of the SHAM, OVX and OVX+ E groups, the c-Fos-positive cell densities all changed in a similar manner: the cell density decreased when the temperature was less than 25 degrees C and the density increased when the temperature was greater than 25 C, demonstrating a V-type curve. The c-Fos density was lowest at 25 degrees C. The other nuclei demonstrated irregular changes. The positive cell densities in the MnPO, AM, and PVN of the SHAM, OVX + E, and OVX+ ICR groups were greater than the densities measured in the OVX group at all temperatures, except 25 degrees C. Positive cell densities in the SHAM, OVX+ E, and OVX+ ICR groups were greater than the densities measured in the OVX groups in the MPA at 25 degrees C, in the VMPO at 4 degrees C, 33 degrees C, and 38 degrees C, in the SO at 4 degrees C, 10 degrees C, and 38 degrees C, and in the SCh at 33 degrees C.

Conclusion: Regardless of the temperature, positive cell densities were lower in the MnPO, MPA, VMPO, AM, SCh, SO, and PVN of the OVX groups in comparison with the densities measured in the same sites in the SHAM group. Following the administration of black cohosh and estrogen, the positive cell densities in the OVX groups increased and became closer to, or exceeded, those measured in the SHAM group, suggesting that both drugs may act on the hypothalamic nuclei and have therapeutic effects on menopausal symptoms. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000307035300023
项目编号81070462 ; 7113168
资助机构Natural Science Foundation of China ; Natural Science Foundation of Beijing
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66896
专题北京大学第一临床医学院
作者单位1.Beijing Univ, Hosp 1, Stat Off, Beijing 100871, Peoples R China
2.Beijing Univ, Hlth Sci Ctr, Dept Anat & Embryol, Beijing 100871, Peoples R China
3.Beijing Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang Hui,Ma Xiaoyan,Yang Mukun,et al. Effects of black cohosh and estrogen on the hypothalamic nuclei of ovariectomized rats at different temperatures[J]. JOURNAL OF ETHNOPHARMACOLOGY,2012,142(3):769-775.
APA Zhang Hui.,Ma Xiaoyan.,Yang Mukun.,Wang Ke.,Yang Liyuan.,...&Bai Wenpei.(2012).Effects of black cohosh and estrogen on the hypothalamic nuclei of ovariectomized rats at different temperatures.JOURNAL OF ETHNOPHARMACOLOGY,142(3),769-775.
MLA Zhang Hui,et al."Effects of black cohosh and estrogen on the hypothalamic nuclei of ovariectomized rats at different temperatures".JOURNAL OF ETHNOPHARMACOLOGY 142.3(2012):769-775.
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