IR@PKUHSC  > 北京大学药学院  > 化学生物学系
学科主题药学
Identification of dicalcium phosphate dihydrate deposited during osteoblast mineralization in vitro
Zhang, Zheng-Lai; Chen, Xiao-Rong; Bian, Sha; Huang, Jian; Zhang, Tian-Lan; Wang, Kui
关键词Osteoblasts Mineralization Dicalcium phosphate dehydrate Octacalcium phosphate
刊名JOURNAL OF INORGANIC BIOCHEMISTRY
2014-02-01
DOI10.1016/j.jinorgbio.2013.11.006
131页:109-114
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TRANSIENT PRECURSOR STRATEGY ; AMORPHOUS CALCIUM-PHOSPHATE ; OCTACALCIUM PHOSPHATE ; CELL-CULTURES ; SOLID TITRATION ; BONE ; MATRIX ; HYDROXYAPATITE ; SOLUBILITY ; CRYSTALS
英文摘要

The hydroxyapatite (HAP) with variable chemical substitutions has been considered as the major component in the mineralized part of bones. Various metastable crystalline phases have been suggested as transitory precursors of HAP in bone, but there are no consensuses as to the nature of these phases and their temporal evolution. In the present study, we cultured rat calvarial osteoblasts with ascorbate and beta-glycerophosphate to explore which calcium phosphate precursor phases comprise the initial mineral in the process of osteoblast mineralization in vitro. At the indicated time points, the deposited calcium phosphate was analyzed after removing organic substances from the extracellular matrix with hydrazine. The features comparable to dicalcium phosphate dihydrate (DCPD) and octacalcium phosphate (OCP), in addition to HAP, were detected in the mineral phases by high resolution transmission electron microscopy. And there was a trend of conversion from DCPD- and OCP-like phases to HAP in the course of mineralization, as indicated by Fourier-transform infrared microspectroscopy, energy-dispersive X-ray spectroscopy and synchrotron X-ray powder diffraction analyses. Besides, biochemical assay showed a progressive decrease in the ratio of mineral-associated proteins to calcium with time. These findings suggest that DCPD- and OCP-like phases are likely to occur on the course of osteoblast mineralization, and the mineral-associated proteins might be involved in modulating the mineral phase transformation. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000329682100015
项目编号21071007 ; 21101008
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China for young
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66917
专题北京大学药学院_化学生物学系
作者单位Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
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GB/T 7714
Zhang, Zheng-Lai,Chen, Xiao-Rong,Bian, Sha,et al. Identification of dicalcium phosphate dihydrate deposited during osteoblast mineralization in vitro[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2014,131:109-114.
APA Zhang, Zheng-Lai,Chen, Xiao-Rong,Bian, Sha,Huang, Jian,Zhang, Tian-Lan,&Wang, Kui.(2014).Identification of dicalcium phosphate dihydrate deposited during osteoblast mineralization in vitro.JOURNAL OF INORGANIC BIOCHEMISTRY,131,109-114.
MLA Zhang, Zheng-Lai,et al."Identification of dicalcium phosphate dihydrate deposited during osteoblast mineralization in vitro".JOURNAL OF INORGANIC BIOCHEMISTRY 131(2014):109-114.
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