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学科主题: 药学
题名:
Identification of dicalcium phosphate dihydrate deposited during osteoblast mineralization in vitro
作者: Zhang, Zheng-Lai; Chen, Xiao-Rong; Bian, Sha; Huang, Jian; Zhang, Tian-Lan; Wang, Kui
关键词: Osteoblasts ; Mineralization ; Dicalcium phosphate dehydrate ; Octacalcium phosphate
刊名: JOURNAL OF INORGANIC BIOCHEMISTRY
发表日期: 2014-02-01
DOI: 10.1016/j.jinorgbio.2013.11.006
卷: 131, 页:109-114
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: TRANSIENT PRECURSOR STRATEGY ; AMORPHOUS CALCIUM-PHOSPHATE ; OCTACALCIUM PHOSPHATE ; CELL-CULTURES ; SOLID TITRATION ; BONE ; MATRIX ; HYDROXYAPATITE ; SOLUBILITY ; CRYSTALS
英文摘要:

The hydroxyapatite (HAP) with variable chemical substitutions has been considered as the major component in the mineralized part of bones. Various metastable crystalline phases have been suggested as transitory precursors of HAP in bone, but there are no consensuses as to the nature of these phases and their temporal evolution. In the present study, we cultured rat calvarial osteoblasts with ascorbate and beta-glycerophosphate to explore which calcium phosphate precursor phases comprise the initial mineral in the process of osteoblast mineralization in vitro. At the indicated time points, the deposited calcium phosphate was analyzed after removing organic substances from the extracellular matrix with hydrazine. The features comparable to dicalcium phosphate dihydrate (DCPD) and octacalcium phosphate (OCP), in addition to HAP, were detected in the mineral phases by high resolution transmission electron microscopy. And there was a trend of conversion from DCPD- and OCP-like phases to HAP in the course of mineralization, as indicated by Fourier-transform infrared microspectroscopy, energy-dispersive X-ray spectroscopy and synchrotron X-ray powder diffraction analyses. Besides, biochemical assay showed a progressive decrease in the ratio of mineral-associated proteins to calcium with time. These findings suggest that DCPD- and OCP-like phases are likely to occur on the course of osteoblast mineralization, and the mineral-associated proteins might be involved in modulating the mineral phase transformation. (C) 2013 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 21071007 ; 21101008
项目资助者: National Natural Science Foundation of China ; National Natural Science Foundation of China for young
WOS记录号: WOS:000329682100015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66917
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Zheng-Lai,Chen, Xiao-Rong,Bian, Sha,et al. Identification of dicalcium phosphate dihydrate deposited during osteoblast mineralization in vitro[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2014,131:109-114.
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