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Selective impairment in glycogen synthase kinase-3 and mitogen-activated protein kinase phosphorylation: comparisons with the hyperandrogenic and the hyperinsulinemic rats
Chen, Yuan; Qiao, Jie; Yan, Li-Ying; Huang, Shuo; Zhao, Pan-Lin; Yan, Jie
关键词Polycystic ovary insulin resistance dehydroepiandrosterone glycogen synthase kinase-3 rat
刊名FERTILITY AND STERILITY
2009-10-01
DOI10.1016/j.fertnstert.2008.08.073
92期:4页:1447-1455
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]POLYCYSTIC-OVARY-SYNDROME ; HUMAN CHORIONIC-GONADOTROPIN ; GROWTH-FACTOR-I ; INSULIN-RESISTANCE ; GLUCOSE-TOLERANCE ; SKELETAL-MUSCLE ; SYNDROME PCOS ; FEMALE RATS ; DEHYDROEPIANDROSTERONE ; WOMEN
英文摘要

Objective: To characterize and compare the effect of DHEA and insulin plus hCG on ovarian morphology, estrous cycle, hormonal levels, insulin sensitivity, and the regulation of insulin signaling in rats.

Design: Animal model study.

Setting: University laboratory.

Animal(s): Female Sprague-Dawley rats.

Intervention(s): Female rats received DHEA or insulin plus hCG by continuous administration.

Main Outcome Measure(s): Ovarian morphology, estrous cycle, hormonal levels, insulin sensitivity, protein levels, and phosphorylation state of glycogen synthase kinase-3 beta and extracellular regulated kinase 1/2 in the ovary.

Result(s): Rats treated with DHEA displayed anovulation, insulin resistance, and polycystic ovaries characterized by cysts and a diminished gramulosa layer. In contrast, insulin plus hCG results in acyclicity with increasing androgen biosynthesis and ovarian morphology different from that in DHEA-treated rats. Moreover, we found that insulin-stimulated serine-phosphorylation of glycogen synthase kinase-3 beta was higher in insulin plus hCG-treated rats but lower in DREA-treated rats. Furthermore, basal and insulin-stimulated tyrosine-phosphorylation of extracellular regulated kinase 1/2 was higher in DHEA-treated rats than in controls.

Conclusion(s): Notwithstanding that both the hyperandrogenism and the hyperinsulinemia synergistic with hCG-treated rats displayed the typical traits of human polycystic ovary syndrome, there is a divergence in the insulin-signaling pathway in the ovarian tissue, which may have a role in the pathogenesis of polycystic ovary syndrome. (Fertil Steril (R) 2009;92:1447-55. (C) 2009 by American Society for Reproductive Medicine.)

语种英语
WOS记录号WOS:000270616100053
项目编号30570872
资助机构Nature Science Foundation of China
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66924
专题北京大学第三临床医学院_生殖医学中心
北京大学第三临床医学院_妇产科
作者单位Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Chen, Yuan,Qiao, Jie,Yan, Li-Ying,et al. Selective impairment in glycogen synthase kinase-3 and mitogen-activated protein kinase phosphorylation: comparisons with the hyperandrogenic and the hyperinsulinemic rats[J]. FERTILITY AND STERILITY,2009,92(4):1447-1455.
APA Chen, Yuan,Qiao, Jie,Yan, Li-Ying,Huang, Shuo,Zhao, Pan-Lin,&Yan, Jie.(2009).Selective impairment in glycogen synthase kinase-3 and mitogen-activated protein kinase phosphorylation: comparisons with the hyperandrogenic and the hyperinsulinemic rats.FERTILITY AND STERILITY,92(4),1447-1455.
MLA Chen, Yuan,et al."Selective impairment in glycogen synthase kinase-3 and mitogen-activated protein kinase phosphorylation: comparisons with the hyperandrogenic and the hyperinsulinemic rats".FERTILITY AND STERILITY 92.4(2009):1447-1455.
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