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Chemiluminescence-based detection technologies for biomolecules, mainly in gel electrophoresis
Xiong, Xin1,2; Ouyang, Jin1; Zhai, Suodi2; Baeyens, Willy R. G.3
关键词Biomolecule Chemiluminescence Detection Direct chemiluminescence detection Enzyme Gel electrophoresis Immunoassay Metallic nanoparticle Nucleic acid Protein
刊名TRAC-TRENDS IN ANALYTICAL CHEMISTRY
2009-09-01
DOI10.1016/j.trac.2009.05.001
28期:8页:961-972
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
资助者National Nature Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Beijing Natural Science Foundation ; National Nature Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Beijing Natural Science Foundation
研究领域[WOS]Chemistry
关键词[WOS]NUCLEIC-ACID LABELS ; POLYACRYLAMIDE-GEL ; TELOMERASE ACTIVITY ; IMAGING DETECTION ; PEROXYOXALATE CHEMILUMINESCENCE ; GOLD NANOPARTICLES ; DNA HYBRIDIZATION ; SERUM-PROTEINS ; PRION PROTEIN ; PEROXIDASE
英文摘要

We provide an overview of powerful applications of chemiluminescence (CL) analysis for biomolecules, mainly in gel electrophoresis. In routine immunciassays, CL labels and detection reactions are widely used for peroxidase and alkali ne-phosphatase enzymes. The sensitivity, the dynamic range and the diversity of CL assays have led to a vast range of applications, notably in protein and nucleic-acid blotting. Non-enzymatic CL detection is also being developed gradually.

Direct CL detection of biomolecules in gels has emerged recently, with simple, convenient and rapid methods. It offers substantial potential to detect many proteins or nucleic acids in complex biological samples. In addition, metallic nanoparticles and catalytic nucleic acids are also potential candidates for CL detection of biomolecules in the future. Crown Copyright (c) 2009 Published by Elsevier Ltd. All rights reserved.

语种英语
所属项目编号20675010 ; 200800270006 ; 2093036
资助者National Nature Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Beijing Natural Science Foundation ; National Nature Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Beijing Natural Science Foundation
WOS记录号WOS:000270249000017
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66952
专题北京大学第三临床医学院_药剂科
作者单位1.Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China
2.Univ Ghent, Fac Pharmaceut Sci, Dept Pharmaceut Anal, B-9000 Ghent, Belgium
3.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xiong, Xin,Ouyang, Jin,Zhai, Suodi,et al. Chemiluminescence-based detection technologies for biomolecules, mainly in gel electrophoresis[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2009,28(8):961-972.
APA Xiong, Xin,Ouyang, Jin,Zhai, Suodi,&Baeyens, Willy R. G..(2009).Chemiluminescence-based detection technologies for biomolecules, mainly in gel electrophoresis.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,28(8),961-972.
MLA Xiong, Xin,et al."Chemiluminescence-based detection technologies for biomolecules, mainly in gel electrophoresis".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 28.8(2009):961-972.
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