北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 解剖学与组织胚胎学系  > 期刊论文
学科主题: 基础医学
题名:
A new in vivo animal model to create intervertebral disc degeneration characterized by MRI, radiography, CT/discogram, biochemistry, and histology
作者: Zhou, HaoWei; Hou, ShuXun; Shang, WeiLin; Wu, WenWen; Cheng, Yao; Mei, Fang; Peng, BaoGan
关键词: 5-bromodeoxyuridine ; animal model ; intervertebral disc degeneration
刊名: SPINE
发表日期: 2007-04-15
卷: 32, 期:8, 页:864-872
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Clinical Neurology ; Orthopedics
研究领域[WOS]: Neurosciences & Neurology ; Orthopedics
关键词[WOS]: LOW-BACK-PAIN ; AGE-RELATED-CHANGES ; MAMMALIAN-CELLS ; NEONATAL EXPOSURE ; NUCLEUS PULPOSUS ; ANNULUS FIBROSUS ; LIFE-SPAN ; 5-BROMODEOXYURIDINE ; HEIGHT ; RATS
英文摘要:

Study Design. A new in vivo sheep model was developed that produced disc degeneration through the injection of 5-bromodeoxyuridine (BrdU) into the intervertebral disc. This process was studied using magnetic resonance imaging (MRI), radiography, CT/discogram, histology, and biochemistry.

Objectives. To develop a sheep model of intervertebral disc degeneration that more faithfully mimics the pathologic hallmarks of human intervertebral disc degeneration.

Summary of Background Data. Recent studies have shown age-related alterations in proteoglycan structure and organization in human intervertebral discs. An animal model that involves the use of age-related changes in disc cells can be beneficial over other more invasive degenerative models that involves directly damaging the matrix of disc tissue.

Methods. Twelve sheep were injected with BrdU or vehicle (phosphate-buffered saline) into the central region of separate lumbar discs. Intact discs were used as controls. At the 2-, 6-, 10-, and 14-week time points, discs underwent MRI, radiography, histology, and biochemical analyses. A CT/discogram study was performed at the 14- week time point.

Results. MRI demonstrated a progressive loss of T2-weighted signal intensity at BrdU-injected discs over the 14- week study period. Radiograph findings included osteophyte and disc space narrowing formed by 10 weeks post-BrdU treatment. CT discography demonstrated internal disc disruption in several BrdU-treated discs at the 14- week time point. Histology showed a progressive loss of the normal architecture and cell density of discs from the 2- week time point to the 14- week time point. A progressive loss of cell proliferation capacity, water content, and proteoglycans was also documented.

Conclusions. BrdU injection into the central region of sheep discs resulted in degeneration of intervertebral discs. This progressive, degenerative process was confirmed using MRI, histology, and by observing changes in biochemistry. Degeneration occurred in a manner that was similar to that observed in human disc degeneration.

语种: 英语
WOS记录号: WOS:000245740500005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66956
Appears in Collections:基础医学院_解剖学与组织胚胎学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
A new in vivo animal model to create intervertebral disc degener.pdf(1185KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.China Peoples Liberat Army Gen Hosp, Affiliated Hosp 2, Dept Orthopaed, Beijing 100037, Peoples R China
2.China Peoples Liberat Army Gen Hosp, Affiliated Hosp 2, Dept Radiol, Beijing 100037, Peoples R China
3.Peking Univ, Sch basic Med Sci, Dept Anat, Beijing 100871, Peoples R China
4.Peking Univ, Sch basic Med Sci, Dept Histol, Beijing 100871, Peoples R China
5.Peking Univ, Sch basic Med Sci, Dept Embryol, Beijing 100871, Peoples R China

Recommended Citation:
Zhou, HaoWei,Hou, ShuXun,Shang, WeiLin,et al. A new in vivo animal model to create intervertebral disc degeneration characterized by MRI, radiography, CT/discogram, biochemistry, and histology[J]. SPINE,2007,32(8):864-872.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, HaoWei]'s Articles
[Hou, ShuXun]'s Articles
[Shang, WeiLin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, HaoWei]‘s Articles
[Hou, ShuXun]‘s Articles
[Shang, WeiLin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace