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学科主题: 药学
题名:
Differentiation of Herba Cistanches by fingerprint with high-performance liquid chromatography-diode array detection-mass spectrometry
作者: Jiang, Y.1; Li, S. R.2; Wang, Y. T.2; Chen, X. J.2; Tu, P. F.1
关键词: Herba Cistanches ; Source differentiation ; Fingerprint ; HPLC-DAD-MS ; Quality control
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-03-13
DOI: 10.1016/j.chroma.2008.04.040
卷: 1216, 期:11, 页:2156-2162
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PHENYLETHANOID GLYCOSIDES ; QUALITY-CONTROL ; CONSTITUENTS ; TUBULOSA
英文摘要:

Herba Cistanche (Rou Cong Rang in Chinese), dried succulent stems of Cistanche deserticola or C. tubulosa, is a famous Chinese herbal medicine and has been recorded in the Chinese Pharmacopoeia. In recent years, another two non-official species, C. salsa and C. sinensis have also been used as Herba Cistanche in some regions of China. To investigate the possibility Of using these two non-official species as alternatives to the official species, a high-performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS) fingerprint method was developed to comparatively analyze the crude herbs of these four species. The fingerprint of C. deserticola, a historically certified species of Herba Cistanche, serves as ′standard pattern′ for comparing the similarities with the other species by means of similarity and Principle Component Analysis. Additionally, 18 characteristic peaks in the fingerprints were identified by comparing their retention times, UV spectra and ESI-MS data with those of the reference substances and/or the data in the literatures. The comparative results demonstrate that the fingerprints of C. tubulosa and C. salsa possess high similarity to the standard pattern, Suggesting that these two species may be used as alternative species; while that of C. sinensis has low similarity (0.053 correlation coefficient) to the standard pattern, indicating that it cannot be used as the substitute of the official herb. However, the varying fingerprint patterns among the samples of C. deserticola collected from various habitats illustrate that the quality consistency of crude herbs is still a problem worthy of serious concern. (C) 2008 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 985-2-102-113
项目资助者: New-Century Talent Program, Ministry of Education of China
WOS记录号: WOS:000264226500024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66975
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100871, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China

Recommended Citation:
Jiang, Y.,Li, S. R.,Wang, Y. T.,et al. Differentiation of Herba Cistanches by fingerprint with high-performance liquid chromatography-diode array detection-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2156-2162.
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