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学科主题: 基础医学
题名:
mutL as a genetic switch of bacterial mutability: turned on or off through repeat copy number changes
作者: Chen, Fang1,2; Liu, Wei-Qiao1,3,4; Liu, Zhen-Hong1,5; Zou, Qing-Hua2; Wang, Ye2; Li, Yong-Guo6; Zhou, Jin6; Eisenstark, Abraham7; Johnston, Randal N.8,9; Liu, Gui-Rong1; Yang, Bao-Feng5; Liu, Shu-Lin1,2,3,4,6
关键词: mutL ; mutability ; Salmonella ; evolution ; genetic switch
刊名: FEMS MICROBIOLOGY LETTERS
发表日期: 2010-11-01
DOI: 10.1111/j.1574-6968.2010.02107.x
卷: 312, 期:2, 页:126-132
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: STRESS-INDUCED MUTAGENESIS ; MISMATCH REPAIR PROTEINS ; ESCHERICHIA-COLI K-12 ; GENOMIC CLEAVAGE MAP ; SALMONELLA-TYPHIMURIUM ; MUTATION-RATES ; DNA-REPAIR ; DIVERSIFICATION ; ENTERITIDIS ; NEISSERIA
英文摘要:

Bacterial adaptation to changing environments can be achieved through the acquisition of genetic novelty by accumulation of mutations and recombination of laterally transferred genes into the genome, but the mismatch repair (MMR) system strongly inhibits both these types of genetic changes. As mutation and recombination do occur in bacteria, it is of interest to understand how genetic novelty may be achieved in the presence of MMR. Previously, we observed associations of a defective MMR genotype, 6bp delta mutL, with greatly elevated bacterial mutability in Salmonella typhimurium. To validate these observations, we experimentally converted the mutL gene between the wild-type and 6bp delta mutL in S. typhimurium and inspected the bacterial mutability status. When 6bp delta mutL was converted to mutL, the originally highly mutable Salmonella strains regained genetic stability; when mutL was converted to 6bp delta mutL, the mutability was elevated 100-fold. Interestingly, mutL cells were found to grow out of 6bp delta mutL cells; the new mutL cells eventually replaced the original 6bp delta mutL population. As conversion between mutL and 6bp delta mutL may occur readily during DNA replication, it may represent a previously unrecognized mechanism to modulate bacterial mutability at the population level, allowing bacteria to respond rapidly to changing environments while minimizing the risks associated with persistent hypermutability.

语种: 英语
所属项目编号: NSFC30970078 ; NSFC30870098 ; 30970119 ; 20092307110001
项目资助者: CIHR ; National Natural Science Foundation of China ; Natural Science Foundation of Heilongjiang Province of China ; Harbin Medical University ; Peking University Health Science Center ; Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP)
WOS记录号: WOS:000283158700004
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66992
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Univ Calgary, Dept Mol Biol, Calgary, AB, Canada
2.Harbin Med Coll, Genom Res Ctr, State Prov Key Labs Biomed Pharmaceut China, Harbin 150081, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
4.Univ Calgary, Dept Microbiol, Calgary, AB, Canada
5.Univ Calgary, Dept Infect Dis, Calgary, AB, Canada
6.Univ Calgary, Dept Biochem, Calgary, AB, Canada
7.Harbin Med Coll, Dept Pharmacol, Harbin 150081, Peoples R China
8.Harbin Med Coll, Hosp 1, Genet Detect Lab, Harbin 150081, Peoples R China
9.Univ Missouri, Canc Res Ctr, Columbia, MO USA

Recommended Citation:
Chen, Fang,Liu, Wei-Qiao,Liu, Zhen-Hong,et al. mutL as a genetic switch of bacterial mutability: turned on or off through repeat copy number changes[J]. FEMS MICROBIOLOGY LETTERS,2010,312(2):126-132.
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