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Molecular cloning and characterization of LAPTM4B, a novel gene upregulated in hepatocellular carcinoma
Shao, GZ; Zhou, RL; Zhang, QY; Zhang, Y; Liu, JJ; Rui, JA; Wei, X; Ye, DX
关键词LAPTM4B hepatocellular carcinoma differential expression promoter analysis
刊名ONCOGENE
2003-08-07
DOI10.1038/sj.onc.1206832
22期:32页:5060-5069
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]HEPATITIS-B-VIRUS ; COMPARATIVE GENOMIC HYBRIDIZATION ; MESSENGER-RNA OVEREXPRESSION ; CYCLIN D1 OVEREXPRESSION ; MEMBRANE-PROTEIN ; C-MET ; SACCHAROMYCES-CEREVISIAE ; MULTIDRUG-RESISTANCE ; P53 MUTATION ; CELL-LINES
英文摘要

Lysosomal-associated protein transmembrane-4 beta (LAPTM4B), a novel gene upregulated in hepatocellular carcinoma (HCC), was cloned using fluorescence differential display, RACE, and RT-PCR. It contains seven exons and encodes a 35-kDa protein with four putative transmembrane regions. Both the N- and C-termini of the protein are proline-rich, and may serve as potential ligands for the SH3 domain. Immunohistochemical analysis localized the protein predominantly to intracellular membranes. Northern blot showed that the LAPTM4B mRNAs were remarkably upregulated in HCC (87.3%) and correlated inversely with differentiation status. LAPTM4B was also overexpressed in many HCC-derived cell tines. It was also highly expressed in fetal livers and certain adult normal tissues including the heart, skeletal muscle, testis, and ovary. Promoter function assays showed a distinct difference in the gene′s activities between BEL7402 and HLE cell lines, suggesting that the transcription factors responsible for regulation of the gene in the two cell lines are different, and that possible negative regulatory cis-elements may exist upstream of the promoter region. It was demonstrated that the N-terminus of LAPTM4B was essential for survival of the cells. Cells harboring the full-length LAPTM4B cDNA expression clone displayed a slightly increased efficiency in colony formation. These results suggest that LAPTM4B is a potential protooncogene, whose overexpression is involved in carcinogenesis and progression of HCC. In normal cells, it may also play important roles such as regulation of cell proliferation and survival.

语种英语
WOS记录号WOS:000184578900014
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被引频次:101[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66996
专题基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Cell Biol & Genet, Beijing 100083, Peoples R China
2.Beijing Union Med Coll Hosp, Dept Basic Surg, Beijing 100032, Peoples R China
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Shao, GZ,Zhou, RL,Zhang, QY,et al. Molecular cloning and characterization of LAPTM4B, a novel gene upregulated in hepatocellular carcinoma[J]. ONCOGENE,2003,22(32):5060-5069.
APA Shao, GZ.,Zhou, RL.,Zhang, QY.,Zhang, Y.,Liu, JJ.,...&Ye, DX.(2003).Molecular cloning and characterization of LAPTM4B, a novel gene upregulated in hepatocellular carcinoma.ONCOGENE,22(32),5060-5069.
MLA Shao, GZ,et al."Molecular cloning and characterization of LAPTM4B, a novel gene upregulated in hepatocellular carcinoma".ONCOGENE 22.32(2003):5060-5069.
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