IR@PKUHSC  > 北京大学公共卫生学院
学科主题公共卫生
Differential cytotoxic effects of denitroaristolochic acid II and aristolochic acids on renal epithelial cells
Liu, Qing1; Wang, Qi1; Yang, Xiuwei2; Shen, Xiaofan1; Zhang, Baoxu1
关键词Denitroaristolochic acid MDCK cells Cytotoxicity Reactive oxygen species (ROS)
刊名TOXICOLOGY LETTERS
2009-01-10
DOI10.1016/j.toxlet.2008.10.020
184期:1页:5-12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]TUBULAR CELLS ; METABOLIC-ACTIVATION ; APOPTOSIS ; KIDNEY ; NEPHROTOXICITY ; NEPHROPATHY ; INDUCTION ; CYTOMETRY ; CHLORIDE ; MERCURY
英文摘要

Aristolochic acids (AAs), naturally occurring nephrotoxins and rodent carcinogens, are commonly found in medicinal plants such as Radix atistolochiae. The toxicity of AAs is believed to be associated with the formation of promutagenic AA-DNA adducts, and it has also been suggested that the nitro group in AAs might be important in the process. In order to investigate the role of the nitro group in AA-mediated cytotoxicity, the effects of denitroaristolochic acid II (dN-AAII), aristolochic acid 11 (AAII) and aristolochic acid I (AAI) on renal tubular epithelial Madin-Darby canine kidney (MDCK) cells were examined and compared. The cytotoxicity of AAI, AAII and dN-AAII was found to be time- and concentration-dependent. As determined by MTT assay, AAI was found to be most toxic in MDCK cells upon exposure for 24, 48 and 72 h. followed by AAII, and dN-AAII showed the least cytotoxicity. The effect of AAI and AAII on the integrity of cell membrane was found to be similar and appeared to be more prominent than that of dN-AAII Based on the results obtained from the flow cytometric analysis, significant apoptosis in MDCK cells was observed with AAI and AAII at as low as 25 mu mol/L following exposure for 24 h, whereas significant apoptosis was induced by dN-AAII at a much higher concentration, 300 mu mol/L, suggesting that both AAI and AAII were significantly more cytotoxic than dN-AAII In addition, the levels of reactive oxygen species (ROS) were increased following treatment with AAI, AAII and dN-AAII at concentrations of 5, 25 and 25 mu mol/L, respectively, for 4 h. The results suggest that the nitro group plays an important role in AA-mediated cytotoxicity in MDCK cells and increased intracellular ROS levels may be associated, at least in part, with the cell injury observed in MDCK cells. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000262544400002
项目编号2002AA2Z343C ; 2004AA2Z3730-07 ; Z0004105040311
资助机构National High Technology Program of China ; Committee of Science and Technology of Beijing, PR China
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67003
专题北京大学公共卫生学院
北京大学公共卫生学院_公共卫生学院
作者单位1.Peking Univ, Sch Publ Hlth, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Liu, Qing,Wang, Qi,Yang, Xiuwei,et al. Differential cytotoxic effects of denitroaristolochic acid II and aristolochic acids on renal epithelial cells[J]. TOXICOLOGY LETTERS,2009,184(1):5-12.
APA Liu, Qing,Wang, Qi,Yang, Xiuwei,Shen, Xiaofan,&Zhang, Baoxu.(2009).Differential cytotoxic effects of denitroaristolochic acid II and aristolochic acids on renal epithelial cells.TOXICOLOGY LETTERS,184(1),5-12.
MLA Liu, Qing,et al."Differential cytotoxic effects of denitroaristolochic acid II and aristolochic acids on renal epithelial cells".TOXICOLOGY LETTERS 184.1(2009):5-12.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Qing]的文章
[Wang, Qi]的文章
[Yang, Xiuwei]的文章
百度学术
百度学术中相似的文章
[Liu, Qing]的文章
[Wang, Qi]的文章
[Yang, Xiuwei]的文章
必应学术
必应学术中相似的文章
[Liu, Qing]的文章
[Wang, Qi]的文章
[Yang, Xiuwei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。