北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
Characterization of the herb-derived components in rats following oral administration of Carthamus tinctorius extract by extracting diagnostic fragment ions (DFIs) in the MSn chromatograms
作者: Chen, Jin-Feng1; Song, Yue-Lin2; Guo, Xiao-Yu1; Tu, Peng-Fei1,2; Jiang, Yong1
刊名: ANALYST
发表日期: 2014-12-21
DOI: 10.1039/c4an01707b
卷: 139, 期:24, 页:6474-6485
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; DATA-PROCESSING TECHNIQUES ; TANDEM MASS-SPECTROMETRY ; CHINESE MEDICINE PRESCRIPTION ; IN-VIVO ; EXTENSION STRATEGY ; RING-FISSION ; IDENTIFICATION ; METABOLITES ; MS/MS
英文摘要:

In this study, a new strategy named extracting diagnostic fragment ions (DFIs) in the MSn chromatograms [E(DFI)(MSCs)-Cs-n] was proposed to rapidly detect and identify the in vivo components derived from the extract of Carthamus tinctorius (ECT), using high performance liquid chromatography coupled with hybrid ion trap-time of flight mass spectrometry. In order to comprehensively summarize the DFIs for the global identification of in vivo constituents of ECT, chemical profiling was carried out, and then the typical metabolic pathways of the primary components were proposed according to their chemical categories, by orally administering representative reference compounds. Based on the proposed metabolic pathways and the fragmentation rules, a list of DFIs was constructed and adopted to differentiate and identify the metabolites from the endogenous substances in the MSn chromatograms of ECT-treated biological samples, in combination with the neutral loss scan mode as a supplement. As a result, a total of 156 compounds were tentatively assigned in vivo, including 63, 73, 50, and 17 components from rat plasma, urine, bile, and feces, respectively, following oral administration of ECT. Deglycosylation, oxidation, methylation, sulfonation, and glucuronidation were observed as the major metabolic pathways for the chemical constituents of ECT, and dehydroxylation was detected at the A-ring of flavones for the first time. The findings suggested that the E(DFI)(MSCs)-Cs-n-based strategy which integrated ideas from single compounds to herbal extracts and from extract chemical profiling to in vivo metabolite profiling, could be used as a reliable tool for rapidly discovering and identifying herb-related constituents in vivo.

语种: 英语
所属项目编号: 81222051 ; 2012ZX09103201-036 ; 2012ZX09301002-002-002 ; 2012ZX09304-005
项目资助者: National Natural Science Foundation for Excellent Young Scholars of China ; National Key Technology R&amp ; D Program "New Drug Innovation" of China
WOS记录号: WOS:000345265300021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67007
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Beijing Univ Chinese Med, Modern Res Ctr Tradit Chinese Med, Beijing 100029, Peoples R China

Recommended Citation:
Chen, Jin-Feng,Song, Yue-Lin,Guo, Xiao-Yu,et al. Characterization of the herb-derived components in rats following oral administration of Carthamus tinctorius extract by extracting diagnostic fragment ions (DFIs) in the MSn chromatograms[J]. ANALYST,2014,139(24):6474-6485.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, Jin-Feng]'s Articles
[Song, Yue-Lin]'s Articles
[Guo, Xiao-Yu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, Jin-Feng]‘s Articles
[Song, Yue-Lin]‘s Articles
[Guo, Xiao-Yu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace