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A new multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay for rapid staphylococcal cassette chromosome mec (SCCmec) typing
Cai, Lin2; Kong, Fanrong1; Wang, Qinning1; Wang, Huiping3; Xiao, Meng4; Sintchenko, Vitali1,5; Gilbert, Gwendolyn L.1,5
刊名JOURNAL OF MEDICAL MICROBIOLOGY
2009-08-01
DOI10.1099/jmm.0.007955-0
58期:8页:1045-1057
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]AUREUS STRAINS ; METHICILLIN-RESISTANCE ; GENETIC DIVERSITY ; IDENTIFICATION ; LOCUS ; ASSIGNMENT ; AUSTRALIA ; VARIANTS ; STRATEGY ; ELEMENT
英文摘要

The aim of this study was to develop a new discriminatory method for MRSA SCCmec typing based on multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay to enable rapid identification and classification of MRSA SCCmec types in a clinical laboratory. Forty-five primer sets and 49 probes were designed and tested in uniplex PCR (uPCR) and mPCR/RLB. Probes were compared in silico to 14 whole-genome sequences and 18 partial SCCmec gene sequences of Staphylococcus aureus and complete genome and partial SCCmec genes of seven non-MRSA strains, including meticillin-susceptible S. aureus and meticillin-resistant coagulase-negative staphylococci. The method was tested on a set of 42 well-characterized reference MRSA strains. It identified all five epidemiologically relevant SCCmec types and 26 subtypes, including established and new subtypes of SCCmec III, IV (eight subtypes each) and V (three subtypes). The discriminatory power of mPCR/RLB SCCmec typing was similar to that of MLST and spa typing (Simpson indices of diversity of 0.916, 0.926 and 0.882, respectively; differences not statistically significant). The application of mPCR/RLB hybridization assay to MRSA SCCmec typing can improve the specificity, discriminatory power and throughput of the typing procedure. The detection of up to 43 mPCR products in a single hybridization assay transforms MRSA SCCmec typing from passive epidemiological library typing into a potential tool for near-real-time infection control surveillance and tracking of MRSA transmission in hospitals.

语种英语
WOS记录号WOS:000268817900009
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67016
专题北京大学第二临床医学院_皮科
作者单位1.Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol, Inst Clin Pathol & Med Res, Westmead, NSW 2145, Australia
2.Peking Univ, Peoples Hosp, Dept Dermatol, Beijing 100044, Peoples R China
3.Tianjin Med Univ, Gen Hosp, Dept Dermatol, Tianjin, Peoples R China
4.Peking Univ, Life Sci Coll, Beijing 100871, Peoples R China
5.Univ Sydney, Western Clin Sch, Westmead, NSW 2145, Australia
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GB/T 7714
Cai, Lin,Kong, Fanrong,Wang, Qinning,et al. A new multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay for rapid staphylococcal cassette chromosome mec (SCCmec) typing[J]. JOURNAL OF MEDICAL MICROBIOLOGY,2009,58(8):1045-1057.
APA Cai, Lin.,Kong, Fanrong.,Wang, Qinning.,Wang, Huiping.,Xiao, Meng.,...&Gilbert, Gwendolyn L..(2009).A new multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay for rapid staphylococcal cassette chromosome mec (SCCmec) typing.JOURNAL OF MEDICAL MICROBIOLOGY,58(8),1045-1057.
MLA Cai, Lin,et al."A new multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay for rapid staphylococcal cassette chromosome mec (SCCmec) typing".JOURNAL OF MEDICAL MICROBIOLOGY 58.8(2009):1045-1057.
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