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学科主题: 基础医学
题名:
Biochemical and biophysical studies on the precursor cells of mouse erythrocytes at different stages
作者: Wang, Jinhua; Kai, Weibo; Sun, Dagong; Yao, Weijuan; Wen, Zongyao; Chien, Shu
关键词: Ca2+ concentration ; cell adhesion ; F-actin ; GATA-1 ; precursor cells
刊名: CELL BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2006
卷: 45, 期:2, 页:147-156
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: TRANSFORM INFRARED-SPECTROSCOPY ; VISCOSITY ; DOMAIN ; K562
英文摘要:

The aim of this research is to study the biochemical and biophysical properties of the precursor cells of mouse erythrocytes at different stages and the molecular mechanisms of their regulation. We investigated the degree of terminal differentiation of splenic erythroblasts obtained from mice during the acute phase of disease caused by the anemia-inducing FVA strain of Friend virus. We analyzed the transcription and protein levels of alpha-globin, beta-globin (erythroid special protein) and GATA-1 (a special erythroid transcription factor). We also have examined the Ca2+ concentration, the distribution and amount of F-actin, important cellular components such as nucleic acids, lipids, and proteins, and the adhesion of precursor cells of RBC at different stages to vascular endothelium. Our results indicated that Ca2+ concentration and the distribution and structure of F-actin changed with the development of proerythroblasts, and that the adhesion rate between the precursor cells and endothelial cells can be correlated with the expression levels of ICAM-1 and P-selectin. These alternations caused changes in biophysical properties of the cell, such as membrane fluidity and deformability.

语种: 英语
WOS记录号: WOS:000238083600003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67018
Appears in Collections:基础医学院_北京大学血液流变中心_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Hemorheol Ctr, Dept Med Phys, Beijing 100083, Peoples R China
2.Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
3.Peking Union Med Coll, Beijing 100050, Peoples R China
4.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

Recommended Citation:
Wang, Jinhua,Kai, Weibo,Sun, Dagong,et al. Biochemical and biophysical studies on the precursor cells of mouse erythrocytes at different stages[J]. CELL BIOCHEMISTRY AND BIOPHYSICS,2006,45(2):147-156.
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