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Trypsin-like protease-active extracellular protein extracts from Porphyromonas gingivalis ATCC 33277 induce apoptosis in bovine aortic endothelial cells
Tian, N.; Ouyang, X. Y.
关键词Porphyromonas gingivalis endothelial cell gingipain apoptosis
刊名JOURNAL OF PERIODONTAL RESEARCH
2010-10-01
DOI10.1111/j.1600-0765.2010.01280.x
45期:5页:650-657
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]EPITHELIAL-CELLS ; CYSTEINE PROTEINASES ; ADHESION ; GINGIPAINS ; PERIODONTITIS ; ANOIKIS ; ATHEROSCLEROSIS ; EXPRESSION ; PLAQUE ; BETA-1-INTEGRIN
英文摘要

Background and Objective:

Certain virulence factors participating in periodontitis may relate to cardiovascular diseases. This study was to evaluate the pro-apoptotic effect of protein extracts from Porphyromonas gingivalis on bovine aortic endothelial cells (BAECs).

Material and Methods:

The BAECs were exposed to trypsin-like protease-active protein extracts from P. gingivalis, and apoptosis was examined by Hoechst 33342 staining, DNA fragmentation assay and cleaved caspase-3 detection. When BAECs were exposed to protein extracts pretreated with trypsin-like protease inhibitor (TLCK), the apoptosis rate was evaluated by Annexin V-propidium iodide staining. To further study the potential mechanism of the pro-apoptotic effect, immunoblotting was used to detect expression of alpha-tubulin, integrin beta 1 and activated ERK1/2 in BAECs treated with protein extracts or cultured in suspension.

Results:

After exposure to the protein extracts, BAECs exhibited loss of cell adhesion and apoptotic cell death. The pro-apoptotic effect could be delayed by TLCK pretreatment. In addition, BAECs treated with protein extracts showed decreased levels of alpha-tubulin, integrin beta 1 and activated ERK1/2. When BAECs were cultured in suspension, ERK1/2 activation was also inhibited, but the percentage decrease in ERK1/2 activation was less than that induced by protein extracts. Moreover, no significantly altered expression of alpha-tubulin was detected in suspended cells.

Conclusion:

Trypsin-like protease-active protein extracts from P. gingivalis could induce apoptosis of BAECs. The destruction of alpha-tubulin and integrin beta 1 and decrease of ERK1/2 activation might contribute to the pro-apoptotic effect of the protein extracts.

语种英语
WOS记录号WOS:000281141500008
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67024
专题北京大学口腔医学院_牙周科
北京大学临床肿瘤学院_药剂科
作者单位Peking Univ, Dept Periodontol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
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GB/T 7714
Tian, N.,Ouyang, X. Y.. Trypsin-like protease-active extracellular protein extracts from Porphyromonas gingivalis ATCC 33277 induce apoptosis in bovine aortic endothelial cells[J]. JOURNAL OF PERIODONTAL RESEARCH,2010,45(5):650-657.
APA Tian, N.,&Ouyang, X. Y..(2010).Trypsin-like protease-active extracellular protein extracts from Porphyromonas gingivalis ATCC 33277 induce apoptosis in bovine aortic endothelial cells.JOURNAL OF PERIODONTAL RESEARCH,45(5),650-657.
MLA Tian, N.,et al."Trypsin-like protease-active extracellular protein extracts from Porphyromonas gingivalis ATCC 33277 induce apoptosis in bovine aortic endothelial cells".JOURNAL OF PERIODONTAL RESEARCH 45.5(2010):650-657.
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