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学科主题: 口腔医学
题名:
Trypsin-like protease-active extracellular protein extracts from Porphyromonas gingivalis ATCC 33277 induce apoptosis in bovine aortic endothelial cells
作者: Tian, N.; Ouyang, X. Y.
关键词: Porphyromonas gingivalis ; endothelial cell ; gingipain ; apoptosis
刊名: JOURNAL OF PERIODONTAL RESEARCH
发表日期: 2010-10-01
DOI: 10.1111/j.1600-0765.2010.01280.x
卷: 45, 期:5, 页:650-657
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: EPITHELIAL-CELLS ; CYSTEINE PROTEINASES ; ADHESION ; GINGIPAINS ; PERIODONTITIS ; ANOIKIS ; ATHEROSCLEROSIS ; EXPRESSION ; PLAQUE ; BETA-1-INTEGRIN
英文摘要:

Background and Objective:

Certain virulence factors participating in periodontitis may relate to cardiovascular diseases. This study was to evaluate the pro-apoptotic effect of protein extracts from Porphyromonas gingivalis on bovine aortic endothelial cells (BAECs).

Material and Methods:

The BAECs were exposed to trypsin-like protease-active protein extracts from P. gingivalis, and apoptosis was examined by Hoechst 33342 staining, DNA fragmentation assay and cleaved caspase-3 detection. When BAECs were exposed to protein extracts pretreated with trypsin-like protease inhibitor (TLCK), the apoptosis rate was evaluated by Annexin V-propidium iodide staining. To further study the potential mechanism of the pro-apoptotic effect, immunoblotting was used to detect expression of alpha-tubulin, integrin beta 1 and activated ERK1/2 in BAECs treated with protein extracts or cultured in suspension.

Results:

After exposure to the protein extracts, BAECs exhibited loss of cell adhesion and apoptotic cell death. The pro-apoptotic effect could be delayed by TLCK pretreatment. In addition, BAECs treated with protein extracts showed decreased levels of alpha-tubulin, integrin beta 1 and activated ERK1/2. When BAECs were cultured in suspension, ERK1/2 activation was also inhibited, but the percentage decrease in ERK1/2 activation was less than that induced by protein extracts. Moreover, no significantly altered expression of alpha-tubulin was detected in suspended cells.

Conclusion:

Trypsin-like protease-active protein extracts from P. gingivalis could induce apoptosis of BAECs. The destruction of alpha-tubulin and integrin beta 1 and decrease of ERK1/2 activation might contribute to the pro-apoptotic effect of the protein extracts.

语种: 英语
所属项目编号: 30471883 ; 2007BAI18B02
项目资助者: National Natural Science Foundation of China ; Ministry of Health PRC
WOS记录号: WOS:000281141500008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67024
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: Peking Univ, Dept Periodontol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Tian, N.,Ouyang, X. Y.. Trypsin-like protease-active extracellular protein extracts from Porphyromonas gingivalis ATCC 33277 induce apoptosis in bovine aortic endothelial cells[J]. JOURNAL OF PERIODONTAL RESEARCH,2010,45(5):650-657.
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