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Metabolic analysis of four phenolic acids in rat by liquid chromatography-tandem mass spectrometry
Zhang, Zi-chuan; Xu, Man; Sun, Shi-feng; Qiao, Xue; Wang, Bao-rong; Han, Jian; Guo, De-an
关键词danshensu caffeic acid ferulic acid isoferulic acid phenolic acid metabolism HPLC-MS
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2008-08-01
DOI10.1016/j.jchromb.2008.06.019
871期:1页:7-14
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]DANSHEN DRIPPING PILLS ; PHASE-II METABOLITES ; SALVIA-MILTIORRHIZA ; CAFFEIC ACID ; COMPONENTS ; IDENTIFICATION ; PRESCRIPTION ; EXTRACT ; ROOTS ; MS
英文摘要

A liquid chromatography-diode array detection-electrospray ionization ion trap mass spectrometry (LC-DAD-ESl-MS(n)) method was established for the analysis of danshensu, caffeic acid, ferulic acid and isoferulic acid in rat plasma, bile, urine and feces after oral administration or intravenous injection. Liquid-liquid extraction was employed for the preparation of biosamples, and the chromatographic separation was carried out using an Agilent Zorbax Extend C(18) reversed phase column and acetonitrile-0.1% formic acid as the mobile phase. Totally nineteen metabolites were detected and identified as prototype, methylated, hydroxylated, sulfated and glucuronized conjugates. The metabolism of the individual phenolic acids in biosamples was investigated, and the metabolic pathway was proposed. By comparing the metabolism of different compounds which shared similar structures, we were able to find that methylation was the main pathway of danshensu metabolism, and the double bond on the side chain was Critical for the drug excretion via bile and the formation of glucuronized conjugates. The results proved that the established method was simple, sensitive and reliable, which could be used to detect and identify the structures of metabolites and to better understand their in vivo metabolism. (C) 2008 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000258584100002
项目编号985-2-063-112 ; 2006BAI08B03-03 ; 104218
资助机构Changjiang Scholars and Innovative Team in University ; Ministry of Science and Technology of China ; Ministry of Education of China
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67030
专题北京大学药学院
作者单位Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Zi-chuan,Xu, Man,Sun, Shi-feng,et al. Metabolic analysis of four phenolic acids in rat by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,871(1):7-14.
APA Zhang, Zi-chuan.,Xu, Man.,Sun, Shi-feng.,Qiao, Xue.,Wang, Bao-rong.,...&Guo, De-an.(2008).Metabolic analysis of four phenolic acids in rat by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,871(1),7-14.
MLA Zhang, Zi-chuan,et al."Metabolic analysis of four phenolic acids in rat by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 871.1(2008):7-14.
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