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学科主题: 药学
题名:
Metabolic analysis of four phenolic acids in rat by liquid chromatography-tandem mass spectrometry
作者: Zhang, Zi-chuan; Xu, Man; Sun, Shi-feng; Qiao, Xue; Wang, Bao-rong; Han, Jian; Guo, De-an
关键词: danshensu ; caffeic acid ; ferulic acid ; isoferulic acid ; phenolic acid ; metabolism ; HPLC-MS
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2008-08-01
DOI: 10.1016/j.jchromb.2008.06.019
卷: 871, 期:1, 页:7-14
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: DANSHEN DRIPPING PILLS ; PHASE-II METABOLITES ; SALVIA-MILTIORRHIZA ; CAFFEIC ACID ; COMPONENTS ; IDENTIFICATION ; PRESCRIPTION ; EXTRACT ; ROOTS ; MS
英文摘要:

A liquid chromatography-diode array detection-electrospray ionization ion trap mass spectrometry (LC-DAD-ESl-MS(n)) method was established for the analysis of danshensu, caffeic acid, ferulic acid and isoferulic acid in rat plasma, bile, urine and feces after oral administration or intravenous injection. Liquid-liquid extraction was employed for the preparation of biosamples, and the chromatographic separation was carried out using an Agilent Zorbax Extend C(18) reversed phase column and acetonitrile-0.1% formic acid as the mobile phase. Totally nineteen metabolites were detected and identified as prototype, methylated, hydroxylated, sulfated and glucuronized conjugates. The metabolism of the individual phenolic acids in biosamples was investigated, and the metabolic pathway was proposed. By comparing the metabolism of different compounds which shared similar structures, we were able to find that methylation was the main pathway of danshensu metabolism, and the double bond on the side chain was Critical for the drug excretion via bile and the formation of glucuronized conjugates. The results proved that the established method was simple, sensitive and reliable, which could be used to detect and identify the structures of metabolites and to better understand their in vivo metabolism. (C) 2008 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 985-2-063-112 ; 2006BAI08B03-03 ; 104218
项目资助者: Changjiang Scholars and Innovative Team in University ; Ministry of Science and Technology of China ; Ministry of Education of China
WOS记录号: WOS:000258584100002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67030
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, Zi-chuan,Xu, Man,Sun, Shi-feng,et al. Metabolic analysis of four phenolic acids in rat by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,871(1):7-14.
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