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Discrimination between peptide and non-peptide opioid agonists on the transcription of opioid receptors in two cell lines
Wang, Y; Wang, XM; Han, JS
关键词peptide opioid agonist non-peptide opioid agonist long-term exposure gene expression opioid receptor
刊名LIFE SCIENCES
2001-05-04
68期:24页:2731-2740
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]GENE-EXPRESSION ; DOWN-REGULATION ; RAT-BRAIN ; CHOLECYSTOKININ ; DESENSITIZATION ; CLONING ; MOUSE ; ACID ; RNA
英文摘要

The aim of the present study was to characterize the effects of prolonged use of peptide mu- and delta -receptor agonists [D-Ala(2), N-me-phe, Gly(5)-ol]-enkephalin (DAMGO) and [D-Pen(2), D-Pen(5)]-enkephalin (DPDPE) and non-peptide agonists ohmefentanyl (OMF) and BW373U86 on the transcription of opioid receptors of cultured NG108-15 cell and SHSY5Y cells, respectively using the method of reverse transcription-polymerase chain reaction (RT-PCR). It was found that (1) The abundance of mu- and delta- receptor mRNA decreased significantly up to 48h after the administration of DAMGO and DPDPE, respectively; whereas the inhibitory effect of OMF and BW373U86 lasted only for 24h; (2) DAMGO and DPDPE produced a significant decrease of the mRNA coding for mu -redeptor and 6-receptor at concentrations as low as 10(-8) mol/L and 10(-6) mol/L, respectively, whereas OMF and BW373U86 were effective at concentrations one order of magnitude higher, respectively. These results suggested that (1) Long-term administration of either peptide or non-peptide opioid agonist to cultured cell line produced a significant decrease of the gene expression of opioid receptor at transcription level. (2) The effect of peptide agonists was stronger and lasted longer than that of corresponding nonpeptide agonists. (C) 2001 Elsevier Science Inc. All rights reserved.

语种英语
WOS记录号WOS:000168709300009
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67035
专题基础医学院_神经生物学系
作者单位Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
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GB/T 7714
Wang, Y,Wang, XM,Han, JS. Discrimination between peptide and non-peptide opioid agonists on the transcription of opioid receptors in two cell lines[J]. LIFE SCIENCES,2001,68(24):2731-2740.
APA Wang, Y,Wang, XM,&Han, JS.(2001).Discrimination between peptide and non-peptide opioid agonists on the transcription of opioid receptors in two cell lines.LIFE SCIENCES,68(24),2731-2740.
MLA Wang, Y,et al."Discrimination between peptide and non-peptide opioid agonists on the transcription of opioid receptors in two cell lines".LIFE SCIENCES 68.24(2001):2731-2740.
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