|Ischemic preconditioning reduces transplanted submandibular gland injury|
|Yang, Ning-Yan3; Shi, Liang4; Zhang, Yan1,2; Ding, Chong5; Cong, Xin1,2; Fu, Feng-Ying1,2; Wu, Li-Ling1,2; Yu, Guang-Yan3|
|关键词||Apoptosis Ischemic preconditioning Reactive oxygen species Submandibular gland Transplantation|
|刊名||JOURNAL OF SURGICAL RESEARCH|
|WOS标题词||Science & Technology|
|关键词[WOS]||REPERFUSION INJURY ; ISCHEMIA/REPERFUSION INJURY ; KERATOCONJUNCTIVITIS SICCA ; LIVER-TRANSPLANTATION ; RAT-LIVER ; CEREBRAL-ISCHEMIA ; HEPATIC ISCHEMIA ; OXIDATIVE DAMAGE ; KAPPA-B ; APOPTOSIS|
Background: Ischemic preconditioning (IPC) can reduce ischemia/reperfusion (I/R) injury in multiple organs and species. However, the effect of IPC on transplanted submandibular glands remains unknown. We explored the protection of IPC in transplanted submandibular glands in the rabbit and the underlying mechanism.
Methods: IPC was performed by clamping the lingual artery for 10 min, with 10 min of reperfusion before transplantation. Male rabbits were randomly divided into control, transplantation, and IPC groups (n = 6 each). Saliva secretion, oxidative stress, pro-inflammatory cytokine levels, and apoptosis-related protein levels were determined at 1, 12, and 24 h after reperfusion.
Results: Salivary flow was significantly increased at 12 h and decreased at 24 h in the transplanted glands. IPC treatment prevented the reduced saliva secretion at 24 h after reperfusion (P < 0.01). The mRNA levels of tumor necrosis factor-alpha, interleukin-1 beta, and reactive oxygen species, as well as malondialodehyde (MDA) and myeloperoxidase activity, were significantly increased and superoxide dismutase activity was decreased in the transplanted glands. However, these changes were all attenuated with IPC treatment (all P < 0.05). Also, acinar cell apoptosis and Bax protein expression were decreased and Bcl-2 protein expression was increased in the IPC-treated glands at 1 and 12 h after reperfusion (all P < 0.05).
Conclusions: IPC protects the secretory function of transplanted submandibular gland in the rabbit by reducing the inflammatory response, attenuating oxidative stress, and an antiapoptosis process. (C) 2013 Elsevier Inc. All rights reserved.
|作者单位||1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China|
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100191, Peoples R China
4.Shandong Univ, Qilu Hosp, Dept Oral & Maxillofacial Surg, Jinan, Shandong, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100191, Peoples R China
|Yang, Ning-Yan,Shi, Liang,Zhang, Yan,et al. Ischemic preconditioning reduces transplanted submandibular gland injury[J]. JOURNAL OF SURGICAL RESEARCH,2013,179(1):E265-E273.|
|APA||Yang, Ning-Yan.,Shi, Liang.,Zhang, Yan.,Ding, Chong.,Cong, Xin.,...&Yu, Guang-Yan.(2013).Ischemic preconditioning reduces transplanted submandibular gland injury.JOURNAL OF SURGICAL RESEARCH,179(1),E265-E273.|
|MLA||Yang, Ning-Yan,et al."Ischemic preconditioning reduces transplanted submandibular gland injury".JOURNAL OF SURGICAL RESEARCH 179.1(2013):E265-E273.|